Compounds and methods for the treatment or prevention of flavivirus infections

ABSTRACT

The present invention provides novel compounds represented by formula I: 
     
       
         
         
             
             
         
       
     
     or pharmaceutically acceptable salts thereof useful for treating flaviviridae viral infection.

FIELD OF THE INVENTION

The present invention relates to novel compounds and a method for the treatment or prevention of Flavivirus infections using novel compounds.

BACKGROUND OF THE INVENTION

Hepatitis is a disease occurring throughout the world. It is generally of viral nature, although there are other causes known. Viral hepatitis is by far the most common form of hepatitis. Nearly 750,000 Americans are affected by hepatitis each year, and out of those, more than 150,000 are infected with the hepatitis C virus (“HCV”).

HCV is a positive-stranded RNA virus belonging to the Flaviviridae family and has closest relationship to the pestiviruses that include hog cholera virus and bovine viral diarrhea virus (BVDV). HCV is believed to replicate through the production of a complementary negative-strand RNA template. Due to the lack of efficient culture replication system for the virus, HCV particles were isolated from pooled human plasma and shown, by electron microscopy, to have a diameter of about 50-60 nm. The HCV genome is a single-stranded, positive-sense RNA of about 9,600 bp coding for a polyprotein of 3009-3030 amino-acids, which is cleaved co and post-translationally by cellular and two viral proteinases into mature viral proteins (core, E1, E2, p7, NS2, NS3, NS4A, NS4B, NS5A, NS5B). It is believed that the structural proteins, E1 and E2, the major glycoproteins are embedded into a viral lipid envelope and form stable heterodimers. It is also believed that the structural core protein interacts with the viral RNA genome to form the nucleocapsid. The nonstructural proteins designated NS2 to NS5 include proteins with enzymatic functions involved in virus replication and protein processing including a polymerase, protease and helicase.

The main source of contamination with HCV is blood. The magnitude of the HCV infection as a health problem is illustrated by the prevalence among high-risk groups. For example, 60% to 90% of hemophiliacs and more than 80% of intravenous drug abusers in western countries are chronically infected with HCV. For intravenous drug abusers, the prevalence varies from about 28% to 70% depending on the population studied. The proportion of new HCV infections associated with post-transfusion has been markedly reduced lately due to advances in diagnostic tools used to screen blood donors.

The only treatment currently available for HCV infection is interferon-α (IFN-α). However, according to different clinical studies, only 70% of treated patients normalize alanine aminotransferase (ALT) levels in the serum and after discontinuation of IFN, 35% to 45% of these responders relapse. In general, only 20% to 25% of patients have long-term responses to IFN. Clinical studies have shown that combination treatment with IFN and ribavirin (RIBA) results in a superior clinical response than IFN alone. Different genotypes of HCV respond differently to IFN therapy, genotype 1b is more resistant to IFN therapy than type 2 and 3.

There is therefore a great need for the development of anti-viral agents.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides novel compounds represented by formula I:

or pharmaceutically acceptable salts thereof; wherein, X is chosen from:

wherein, M is chosen from:

a bond; wherein, R₄ is C₁₋₆ alkyl; R₈ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₂ heteroaralkyl, C₆₋₁₆ aralkyl; and R₁₅ is chosen from H or C₁₋₆ alkyl; J is chosen from:

wherein W is chosen from O, S or NR₇,

-   -   wherein R₇ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂         alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₂ heteroaralkyl,         C₆₋₁₆ aralkyl;         and R₆ is chosen from H, C₁₋₁₂ alkyl, C₆₋₁₄ aryl or C₆₋₁₆         aralkyl;         Y¹ is chosen from a bond, C₁₋₆ alkyl, C₂₋₆ alkenyl or C₂₋₆         alkynyl;         Y is chosen from COOR₁₆, COCOOR₅, P(O)OR_(a)OR_(b), S(O)OR₅,         S(O)₂OR₅, tetrazole, CON(R₉)CH(R₅)COOR₅, CONR₁₀R₁₁,         CON(R₉)—SO₂—R₅, CONR₉OH or halogen, wherein R₉, R₅, R₁₀ and

R₁₁ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl;

or R₁₀ and R₁₁ are taken together with the nitrogen to form a 3 to 10 membered heterocycle; R_(a) and R_(b) are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl and C₆₋₁₈ aralkyl; or R_(a) and R_(b) are taken together with the oxygens to form a 5 to 10 membered heterocycle; R₁₆ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl and C₆₋₁₈ aralkyl; provided that R₁₆ is other than methyl or ethyl; R₁ is chosen from C₂₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl or C₆₋₁₈ aralkyl; R₂ is chosen from C₂₋₁₂ alkyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈ aralkyl; R₃ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl or C₆₋₁₈ aralkyl; Z is chosen from H, halogen, C₁₋₆ alkyl; with the proviso that: i) when X is 4-Chloro-2,6-dimethyl-benzenesulfonamide and, R₁ is phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #580 ii) when X is Toluene-4-sulfonamide and R₁ is 4-chloro-phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #563 iii) when X is Toluene-4-sulfonamide and R₁ is 4-fluoro-phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #564 iv) when X is Toluene-4-sulfonamide and R₁ is 4-methoxy-phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #565 v) when X is Benzamide and R₁ is phenyl Y¹ is a bond and Y is COOH then R₃ is other than hydrogen.

The compounds of the present invention are useful in therapy, particularly as antivirals.

In another aspect, there is provided a method of treating viral infections in a subject in need of such treatment comprising administering to the subject a therapeutically effective amount of a compound of formula (I) or composition of the invention.

In still another aspect, there is provided a method of treating viral infections in a subject in need of such treatment comprising administering to the subject a combination comprising at least one compound of formula (I) and at least one further therapeutic agent.

In another aspect, there is provided a pharmaceutical formulation comprising the compound of the invention in combination with a pharmaceutically acceptable carrier or excipient. Another aspect of the invention is the use of a compound according to formula (I), for the manufacture of a medicament for the treatment of viral infections.

In another aspect, there is provided a method for inhibiting or reducing the activity of viral polymerase in a host comprising administering a therapeutically effective amount of a compound of formula (I).

DETAILED DESCRIPTION OF THE INVENTION

In one embodiment, compounds of the present invention comprise those wherein the following embodiments are present, either independently or in combination.

In one embodiment, the present invention provides novel compounds of formula (Ia):

or pharmaceutically acceptable salts thereof; wherein, X is chosen from:

wherein, M is chosen from:

a bond; wherein, R₄ is C₁₋₆ alkyl; R₈ is chosen from H, C₁₋₁₂ aryl, C₃₋₁₂ heterocycle, C₃₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ heteroaralkyl, C₆₋₁₆ aralkyl; and R₁₅ is chosen from H or C₁₋₆ alkyl; J is chosen from:

wherein W is chosen from O, S or NR₇,

-   -   wherein R₇ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂         alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₂ heteroaralkyl,         C₆₋₁₆ aralkyl;         and R₆ is chosen from H, C₁₋₁₂ alkyl, C₆₋₁₄ aryl or C₆₋₁₆         aralkyl;         Y¹ is chosen from a bond, C₁₋₆ alkyl, C₂₋₆ alkenyl or C₂₋₆         alkynyl;         Y is chosen from COOR₁₆, COCOOR₅, P(O)OR_(a)OR_(b), S(O)OR₅,         S(O)₂OR₅, tetrazole, CON(R₉)CH(R₅)COOR₅, CONR₁₀R₁₁,         CON(R₉)—SO₂—R₅, CONR₉OH or halogen, wherein R₉, R₅, R₁₀ and         R₁₁ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂         alkenyl, C₂₋₁₂ alkynyl, C₃₋₁₂ heterocycle, C₃₋18 heteroaralkyl,         C₆₋₁₈ aralkyl;         or R₁₀ and R₁₁ are taken together with the nitrogen to form a 3         to 10 membered heterocycle;         R_(a) and R_(b) are each independently chosen from H, C₁₋₁₂         alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂         heterocycle, C₃₋₁₈ heteroaralkyl and C₆₋₁₈ aralkyl;         or R_(a) and R_(b) are taken together with the oxygens to form a         5 to 10 membered heterocycle;         R₁₆ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl,         C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl and C₆₋₁₈         aralkyl; provided that R₁₆ is other than methyl or ethyl;         R₁ is chosen from C₂₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl,         C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl or C₆₋₁₈         aralkyl;         R₂ is chosen from C₂₋₁₂ alkyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂         heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈ aralkyl;         R₃ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl,         C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl or C₆₋₁₈         aralkyl;         Z is chosen from H, halogen, C₁₋₆ alkyl; with the proviso that:         i) when X is 4-Chloro-2,6-dimethyl-benzenesulfonamide and, R₁ is         phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than         CONH₂; compound #580         ii) when X is Toluene-4-sulfonamide and R₁ is 4-chloro-phenyl,         and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂;         compound #563         iii) when X is Toluene-4-sulfonamide and R₁ is 4-fluoro-phenyl,         and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂;         compound #564         iv) when X is Toluene-4-sulfonamide and R₁ is 4-methoxy-phenyl,         and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂;         compound #565         v) when X is Benzamide and R₁ is phenyl Y¹ is a bond and Y is         COOH then R₃ is other than hydrogen.

In a further aspect, the present invention provides novel compounds represented by formula II:

and pharmaceutically acceptable salts thereof, wherein, M is chosen from:

a bond; Y¹ is chosen from a bond, C₁₋₆ alkyl, C₂₋₆ alkenyl or C₂₋₆ alkynyl; Y is chosen from COOR₁₆, CO—COOR₅, PO₃R_(a)R_(b), SO₃R₅, tetrazole, CON(R₉)CH(R₅)—COOR₅, CON R₁₀R₁₁ or CONR₉OH, wherein

-   -   each R₅ R₉, R₁₀, R₁₁, R₁₆, R_(a), and R_(b) are independently         chosen from H or C₁₋₆ alkyl;         R₁ is chosen from C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂         aryl, C₃₋₁₀ heterocycle, C₃₋₁₀ heteroaralkyl, C₆₋₁₂ aralkyl, or         a halogen;         R₂ is chosen from C₆₋₁₂ aryl, C₃₋₁₀ heterocycle, C₆₋₁₂ aralkyl         or C₃₋₁₀ heteroaralkyl;         R₃ is chosen from H or C₁₋₆ alkyl; C₆₋₁₂ aralkyl or C₃₋₁₀         heteroaralkyl;         R₄ is chosen from H or C₁₋₆ alkyl;         R₁₅ is chosen from H or C₁₋₆ alkyl         with the proviso that:         i) when M is

and R₂ is 4-chloro-2,5-dimethyl-phenyl, R₁ is phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #580 ii) when M is

and R₂ is 4-methylphenyl, R₁ is 4-chloro-phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #563 iii) when M is

and R₂ is 4-methylphenyl, R₁ is 4-fluoro-phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #564

iv) when M is and R₂ is 4-methylphenyl, R₁ is 4-methoxy-phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #565

In still a further embodiment, the present invention provides novel compounds of formula (IIa):

wherein, M is chosen from:

a bond; Y¹ is chosen from a bond, C₁₋₆ alkyl, C₂₋₆ alkenyl or C₂₋₆ alkynyl; Y is chosen from COOR₁₆, CO—COOR₅, PO₃R_(a)R_(b), SO₃R₅, tetrazole, CON(R₉)CH(R₅)—COOR₅, CON R₁₀R₁₁ or CONR₉OH, wherein each R₅ R₉, R₁₀, R₁₁, R₁₆, R_(a), and R_(b) are independently chosen from H or C₁₋₆ alkyl; R₁ is chosen from C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aryl, C₃₋₁₀ heterocycle, C₃₋₁₀ heteroaralkyl, C₆₋₁₂ aralkyl, or a halogen; R₂ is chosen from C₆₋₁₂ aryl, C₃₋₁₀ heterocycle, C₆₋₁₂ aralkyl or C₃₋₁₀ heteroaralkyl; R₃ is chosen from H or C₁₋₆ alkyl; C₆₋₁₂ aralkyl or C₃₋₁₀ heteroaralkyl; R₄ is chosen from H or C₁₋₆ alkyl; R₁₅ is chosen from H or C₁₋₆ alkyl; with the proviso that: i) when M is

and R₂ is 4-chloro-2,5-dimethyl-phenyl, R₁ is phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #580 ii) when M is

and R₂ is 4-methylphenyl, R₁ is 4-chloro-phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #563 iii) when M is

and R₂ is 4-methylphenyl, R₁ is 4-fluoro-phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #564

iv) when M is and R₂ is 4-methylphenyl, R₁ is 4-methoxy-phenyl, and R₃ is H, and Y¹ is a bond, then Y is other than CONH₂; compound #565.

In one embodiment, X is:

In a further embodiment, X is:

In one embodiment, Z is chosen from H, halogen, C₁₋₆ alkyl.

In further embodiments,

Z is H

Z is halogen Z is fluoride Z is C₁₋₆ alkyl Z is chosen from methyl, trifluoromethyl, ethyl, propyl, isopropyl, cyclopropyl, butyl, isobutyl, cyclobutyl, pentyl, neopentyl, cyclopentyl, hexyl or cyclohexyl.

In further embodiments;

R₁ is chosen from C₂₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl or C₆₋₁₈ aralkyl. R₁ is chosen from a C₂₋₁₂ alkyl, C₆₋₁₄ aryl or C₃₋₁₂ heterocycle. R₁ is a C₂₋₁₂ alkyl. R₁ is a C₆₋₁₄ aryl. R₁ is a C₃₋₁₂ heterocycle. R₁ is chosen from t-butyl, isobutyl, allyl, ethynyl, 2-phenylethenyl, isobutenyl, benzyl, phenyl, phenethyl, benzodioxolyl, thienyl, thiophenyl, pyridinyl, isoxazolyl, thiazolyl, pyrazolyl, tetrazolyl, benzofuranyl, indolyl, furanyl, or benzothiophenyl any of which can be optionally substituted by one or more substituent chosen from halogen, nitro, nitroso, SO₂R₁₂, PO₃RcRd, CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; wherein R₁₂, Rc, Rd, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or Rc and Rd are taken together with the oxygens to form a 5 to 10 membered heterocycle; or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle. R₁ is chosen from thienyl, t-butyl, phenyl or pyridinyl. R₁ is isoxazolyl substituted by at least one methyl. R₁ is pyridinyl.

In one embodiment, R₁ is chosen from a C₁₋₆ alkyl, C₆₋₁₂ aryl or C₃₋₁₀ heterocycle.

In one embodiment, R₁ is chosen from t-butyl, isobutyl, allyl, ethynyl, 2-phenylethenyl, isobutenyl, benzyl, phenyl, phenethyl, benzodioxolyl, thienyl, thiophenyl, pyridinyl, isoxazolyl, thiazolyl, pyrazolyl, tetrazolyl, benzofuranyl, indolyl, furanyl, or benzothiophenyl, any of which can be substituted by at least one substituent chosen from C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀ heterocycle, halogen, nitro, CONR₁₃R₁₄, NR₁₃R₁₄, amidino, guanido, Cyano, SO₂—C₁₋₆ alkyl, C(O)OR₁₂, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, or C₆₋₁₂ aryloxy;

wherein R₁₂, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle.

In one embodiment, R₁ is chosen from thienyl, t-butyl, phenyl, thiophenyl, pyridinyl, isoxazolyl, any of which can be substituted by at least one substituent chosen from a halogen, C₁₋₆ alkyl, C₁₋₆ alkyloxy, C₂₋₆ alkenyl, C₂₋₆ alkynyl, nitro, cyano, SO₂—C₁₋₆ alkyl, NO—C₁₋₆ alkyl.

In further embodiments;

R₁ is phenyl. R₁ is phenyl substituted with fluoride. R₁ is phenyl substituted with at least one fluoride R₁ is phenyl di-substituted with fluoride. R₁ is phenyl substituted with chloride. R₁ is phenyl substituted with at least one chloride R₁ is phenyl di-substituted with chloride. R₁ is phenyl substituted with fluoride and chloride. R₁ is phenyl substituted with nitro. R₁ is phenyl substituted with at least one nitro.

R₁ is phenyl substituted with methoxy.

R₁ is phenyl substituted with OCF₃. R₁ is phenyl substituted with CF₃. R₁ is phenyl substituted with methyl. R₁ is phenyl substituted with at least one methyl. R₁ is phenyl substituted with CN. R₁ is phenyl substituted with SO₂—CH₃. R₁ is phenyl substituted with NH(CO)—CH₃.

In further embodiments,

R₁ is thiophenyl. R₁ is thiophenyl substituted by at least one halogen. R₁ is thiophenyl substituted by at least one chloride. R₁ is thiophenyl substituted by at least one methyl. R₁ is thiophenyl substituted by at least one methyl and one chloride.

In further embodiments,

R₁ is thienyl. R₁ is thienyl substituted by at least one halogen. R₁ is thienyl substituted by at least one chloride. R₁ is thienyl substituted by at least one methyl. R₁ is thienyl substituted by at least one methyl and one chloride. R₁ is isoxazole di-substituted with CH₃. R₁ is pyridine.

In one embodiment, M is chosen from:

a bond;

In a further embodiment, M is:

In an alternative embodiment, M is:

In one embodiment, J is chosen from:

wherein, W is as defined above.

In an alternative embodiment, J is:

In a further embodiment, J is:

In one embodiment, Y is chosen from COOR₁₆, COCOOR₅, P(O)OR_(a)OR_(b), S(O)₂OR₅, tetrazole, CON(R₉)CH(R₅)COOR₅, CONR₁₀R₁₁, CONR₉OH.

In a further embodiment, any of R₅, Ra, Rb, R₉, R₁₀, R₁₁ and R₁₆ are each independently chosen from H or C₁₋₆ alkyl; provided that R₁₆ is other than methyl or ethyl.

In one embodiment, Y is chosen from COOR₁₆, CONR₁₀R₁₁ or CON(R₉)CH(R₅)—COOR₅.

In a further embodiment, any of R₅, R₉, R₁₀, R₁₁ and R₁₆ are each independently chosen from H or C₁₋₆ alkyl; provided that R₁₆ is other than methyl or ethyl.

In a further embodiment, Y is chosen from COOR₁₆, CONR₁₀R₁₁ or CONR₉CH₂COOR₅.

In a further embodiment, Y is chosen from COOR₅, CONR₅R₅ or CON(R₅)CH(R₅)_COOR₅.

In a further embodiment, Y is COOH.

In a further embodiment, Y is CONH₂.

In a further embodiment, Y is CONHCH₂COOH.

In a further embodiment, Y is COOCH₃.

In a further embodiment, Y¹ is chosen from CH₂, C═CH, CH—CH₂ or a bond.

In further embodiments;

R₃ is chosen from H, C₁₋₁₂ alkyl, C₆₋₁₈ aralkyl, C₃₋₁₂ heterocycle or C₃₋₁₈ heteroaralkyl. R₃ is chosen from H, C₁₋₁₂ alkyl, C₆₋₁₈ aralkyl or C₃₋₁₂ heterocycle. R₃ is C₁₋₁₂ alkyl. R₃ is C₆₋₁₈ aralkyl. R₃ is C₃₋₁₂ heterocycle. R₃ is chosen from H, methyl, ethyl, i-propyl, cyclopropyl, cyclohexyl, allyl, piperidinyl, piperazinyl, pyrrolidinyl, azetidinyl, aziridinyl, pyridinyl, piperidinylmethyl, dioxanyl, dioxolanyl, azepanyl or benzyl; any of which can be optionally substituted by one or more substituent chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃RcRd, CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; wherein R₁₂, Rc, Rd, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or Rc and Rd are taken together with the oxygens to form a 5 to 10 membered heterocycle; or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle. R₃ is chosen from H or Methyl, isopropyl, piperidinyl, piperidinylmethyl, dioxolanyl or cyclohexyl.

In a further embodiment, R₃ is H or methyl.

In a further embodiment, R₃ is H.

In a further embodiment, R₃ is methyl.

In a further embodiment, R₃ is benzyl, thiophenylmethyl, furanylmethyl.

In additional embodiments;

R₂ is C₂₋₁₂ alkyl, C₆₋₁₄ aryl or C₃₋₁₂ heterocycle; R₂ is C₃₋₆ heterocycle. R₂ is chosen from thienyl, furanyl, pyridinyl, oxazolyl, thiazolyl, pyrrolyl, benzofuranyl, indolyl, benzoxazolyl, benzothienyl, benzothiazolyl, piperazinyl, pyrrolidinyl or quinolinyl any of which can be optionally substituted by one or more substituent chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃RcRd, CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; wherein R₁₂, Rc, Rd, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or Rc and Rd are taken together with the oxygens to form a 5 to 10 membered heterocycle; or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle. R₂ is a heterocycle chosen from thienyl, furanyl, pyridinyl, pyrrolyl, indolyl, piperazinyl or benzothienyl. R₂ is C₂₋₁₂ alkyl. R₂ is chosen from cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl cyclohexyl, cycloheptyl, 2-(cyclopentyl)-ethyl, methyl, ethyl, vinyl, propyl, propenyl, isopropyl, butyl, butenyl isobutyl, pentyl, neopentyl or t-butyl any of which can be optionally substituted by one or more substituent chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃RcRd, CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; wherein R₁₂, Rc, Rd, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or Rc and Rd are taken together with the oxygens to form a 5 to 10 membered heterocycle; or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle. R₂ is C₆₋₁₂ aryl. R₂ is an aryl chosen from indenyl, naphthyl or biphenyl. R₂ is phenyl substituted by one or more substituent chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃RcRd, CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; wherein R₁₂, Rc, Rd, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or Rc and Rd are taken together with the oxygens to form a 5 to 10 membered heterocycle;

or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle.

R₂ is phenyl substituted by one or two substituents chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃RcRd, CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; wherein R₁₂, Rc, Rd, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or Rc and Rd are taken together with the oxygens to form a 5 to 10 membered heterocycle; or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle. R₂ is phenyl substituted by one or more substituent chosen from halogen, nitro, CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₁₋₆ alkyloxy, C(O)C₁₋₆ alkyl, C₆₋₁₂ aryl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, wherein R₁₂, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle. R₂ is phenyl substituted by one or two substituents chosen from halogen, nitro, CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₁₋₆ alkyloxy, C(O)C₁₋₆ alkyl, C₆₋₁₂ aryl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, wherein R₁₂, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle. R₂ is phenyl substituted by one or two substituents chosen from halogen, C₁₋₆ alkyl, NR₁₃R₁₄, nitro, CONR₁₃R₁₄, C(O)OC₁₋₆ alkyl, COOH or C₁₋₆ alkyloxy C(O)OR₁₂, cyano, azido, wherein R₁₂, R₁₃ and R₁₄ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle.

In one embodiment, R₂ is chosen from C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₆₋₁₂ aralkyl or C₃₋₁₀ heteroaralkyl.

In a further embodiment, R₂ is chosen from a C₆₋₁₂ aryl or C₃₋₁₀ heterocycle.

In a further embodiment, R₂ is a C₆ aryl or a C₃₋₆ heterocycle.

In a further embodiment, R₂ is chosen from phenyl, pyridinyl, thiophenyl, benzofuran, thiazole, pyrazole, substituted with at least one substituent chosen from a halogen, C₁₋₆ alkyl, C₁₋₆ alkyloxy, CF₃, COOH, COOC₁₋₆ alkyl, cyano, NH₂, nitro, NH(C₁₋₆ alkyl), N(C₁₋₆ alkyl)₂ or a C₃₋₈ heterocycle.

R₂ is chosen from thienyl, furanyl, pyridyl, oxazolyl, thiazolyl, pyrrolyl, benzofuranyl, indolyl, benzoxazolyl, benzothienyl, benzothiazolyl or quinolinyl any of which can be substituted by at least one substituent chosen from C₁₋₆ alkyl, amino, halogen, nitro, amido, CN, COOC₁₋₆ alkyl, or C₁₋₆ alkyloxy. R₂ is methylphenyl. R₂ is dichlorophenyl.

In a further embodiment, R₂ is chosen from:

wherein: Rw is H, O or methyl; Ry is H or methyl;

Rw is H;

Rw is methyl;

Ry is H;

Ry is methyl; and wherein, Xa is S, N, O or carbon.

In a further embodiment, each of Ra, Rb, Rc, Rd, Re, and Rf are independently chosen from, H, Cl, Br, I, F, C₁₋₆ alkyl, OC₁₋₆ alkyl, CF₃, COOH, COOC₁₋₆ alkyl, CN, NH₂, NO₂, NH(C₁₋₆ alkyl), N(C₁₋₆ alkyl)₂.

In a further embodiment, each of Ra, Rb, Rc, Rd, Re, and Rf are independently chosen from, H, Cl, Br, I, F, methyl, O-methyl, CF₃, COOH, COOCH₃, CN, NH₂, NO₂, NH(CH₃) or N(CH₃)₂.

In a further embodiment, each of Ra, Rb, Rc, Rd, Re, and Rf are independently chosen from, H, Cl, Br, I, F, methyl, O-methyl, CF₃, COOH, COOCH₃, CN, NH₂, or NO₂

In a further embodiment, each of Ra, Rb, Rc, Rd, Re, and Rf are independently chosen from, H, Cl, methyl, O-methyl, CF₃, COOH, COOCH₃, CN, NH₂, or NO₂.

In a further embodiment, each of Ra, Rb, Rc, Rd, Re, and Rf are independently chosen from, H, Cl, F, methyl, CF₃ or O-methyl.

In one embodiment, Rf is H or methyl.

In another embodiment, Rf is H.

In another embodiment, Rf is methyl.

In a further embodiment, each of Ra, Rb, Rc, Rd and Re is independently chosen from, H or Cl.

In a further embodiment, each of Ra, Rb, Rc, Rd and Re is H.

In one embodiment:

Ra is chosen from Cl, F, methyl or O-methyl;

Rb is H;

Rc is chosen from Cl, F, methyl or O-methyl;

Rd is H;

Re is chosen from Cl, F, methyl or O-methyl.

In one embodiment:

Ra is methyl;

Rb is H; Rc is Cl; Rd is H;

Re is methyl.

In a further embodiment, each of Rs, Rt, Ru, are independently chosen from, H, Cl, Br, I, F, C₁₋₆ alkyl, OC₁₋₆ alkyl, CF₃, COOH, COOC₁₋₆ alkyl, CN, NH₂, NO₂, NH(C₁₋₆ alkyl), N(C₁₋₆ alkyl)₂.

In a further embodiment, each of Rs, Rt, Ru, are independently chosen from, H, Cl, Br, I, F, methyl, O-methyl, CF₃, COOH, COOCH₃, CN, NH₂, NO₂, NH(CH₃) or N(CH₃)₂.

In a further embodiment, each of Rs, Rt, Ru, are independently chosen from, H, Cl, Br, I, F, methyl, O-methyl, CF₃, COOH, COOCH₃, CN, NH₂, or NO₂

In a further embodiment, each of Rs, Rt, Ru, are independently chosen from, H, Cl, Br, I, F, methyl, O-methyl, CF₃, COOH, COOCH₃, CN, NH₂, or NO₂.

In a further embodiment, each of Rs, Rt, Ru, are independently chosen from, H, Cl, methyl, O-methyl, CF₃, COOH, COOCH₃, CN, NH₂, or NO₂.

In a further embodiment, each of Rs, Rt, Ru, are independently chosen from, H, Cl, F, methyl, CF₃ or O-methyl.

In a further embodiment, each of Rs, Rt, Ru, are independently chosen from, H or Cl.

In a further embodiment, each of Rs, Rt, Ru, are H.

In one embodiment:

Rs and Ru are Cl and Rt is H. Rs is Cl, Rt and Ru are H.

In one embodiment, the viral infection is chosen from Flavivirus infections.

In one embodiment, the Flavivirus infection is chosen from Hepatitis C virus (HCV), bovine viral diarrhea virus (BVDV), hog cholera virus and yellow fever virus.

In another embodiment, the Flavivirus infection is Hepatitis C viral infection.

In one embodiment, there is provided a method for treating or preventing a Flaviviridae viral infection in a host comprising administering to the host a therapeutically effective amount of at least one compound of formula (III)

or pharmaceutically acceptable salts thereof; wherein, X is chosen from:

wherein, M is chosen from:

a bond; wherein, R₄ is chosen from H or C₁₋₆ alkyl; R₈ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₂ heteroaralkyl, C₆₋₁₆ aralkyl; and R₁₅ is chosen from H or C₁₋₆ alkyl; J is chosen from:

wherein W is chosen from O, S or NR₇,

-   -   wherein R₇ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂         alkynyl, C₆₋₁₂ aryl, C₃₋₁₂ heterocycle, C₃₋₁₂ heteroaralkyl,         C₆₋₁₆ aralkyl;         and R₆ is chosen from H, C₁₋₁₂ alkyl, C₆₋₁₂ aryl or C₆₋₁₆         aralkyl;         Y¹ is chosen from a bond, C₁₋₆ alkyl, C₂₋₆ alkenyl or C₂₋₆         alkynyl;         Y is chosen from COOR₁₆, COCOOR₅, P(O)OR_(a)OR_(b), S(O)OR₅,         S(O)₂OR₅, tetrazole, CON(R₉)CH(R₅)COOR₅, CONR₁₀R₁₁,         CON(R₉)—SO₂—R₅, CONR₉OH or halogen, wherein R₉, R₅, R₁₀ and R₁₁         are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂         alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈         heteroaralkyl, C₆₋₁₈ aralkyl;         or R₁₀ and R₁₁ are taken together with the nitrogen to form a 3         to 10 membered heterocycle;         R_(a) and R_(b) are each independently chosen from H, C₁₋₁₂         alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂         heterocycle, C₃₋₁₈ heteroaralkyl and C₆₋₁₈ aralkyl;         or R_(a) and R_(b) are taken together with the oxygens to form a         5 to 10 membered heterocycle;         R₁₆ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl,         C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl and C₆₋₁₈         aralkyl;         R₁ is chosen from C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl,         C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈         aralkyl, or halogen;         R₂ is chosen from C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl,         C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈         aralkyl;         R₃ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl,         C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl or C₆₋₁₈         aralkyl;         Z is chosen from H, halogen, C₁₋₆ alkyl.

In one embodiment, there is provided a method for treating or preventing Flaviviridae viral infection in a host comprising administering to the host a therapeutically effective amount of at least one compound of formula (III), further comprising at least one antiviral agent.

In one embodiment, the antiviral agent is chosen from a viral serine protease inhibitor, viral polymerase inhibitor and viral helicase inhibitor.

In a further embodiment, the antiviral agent is chosen from interferon α and ribavirin.

In a further embodiment, said compound of formula (III) and said antiviral agent are administered sequentially.

In a further embodiment, said compound of formula (III) and said antiviral agent are administered simultaneously.

In one embodiment, there is provided a method for treating or preventing Flaviviridae viral infection in a host comprising administering to the host a therapeutically effective amount of at least one compound of formula (III) and at least one additional agent chosen from immunomudulating agent, antioxydant agent, antibacterial agent or antisense agent.

In another embodiment, the additional agent is chosen from silybum marianum, interleukine-12, amantadine, ribozyme, thymosin, N-acetyl cysteine or cyclosporin.

In further embodiments;

The compound of formula (III) and the additional agent are administered sequentially. The compound of formula (III) and the additional agent are administered simultaneously.

In one embodiment, the present invention further provides A pharmaceutical composition comprising at least one compound having the formula III or pharmaceutically acceptable salts thereof; and at least one pharmaceutically acceptable carrier or excipient.

In a further embodiment, the pharmaceutical composition, is further comprising one or more additional agent chosen from antiviral agent, immunomudulating agent, antioxydant agent, antibacterial agent or antisense agent.

In one embodiment, the antiviral agent is chosen from a viral serine protease inhibitor, viral polymerase inhibitor and viral helicase inhibitor.

In one embodiment, the antiviral agent is chosen from interferon α and ribavirin.

In one embodiment, the additional agent is chosen from silybum marianum, interleukine-12, amantadine, ribozyme, thymosin, N-acetyl cysteine or cyclosporin.

In one embodiment, the invention further provides the use of a compound having the formula III for the manufacture of a medicament for treating or preventing a viral Flaviridea infection in a host

In one embodiment, there is provided the use of a compound having the formula III or pharmaceutically acceptable salts thereof in therapy

In one embodiment, the invention provides the use of a compound having the formula III for treating or preventing Flaviviridae viral infection in a host.

In one embodiment, the use of a compound having the compound of formula III for treating or preventing Flaviviridae viral infection in a host is further comprising one or more additional agent chosen from antiviral agent, immunomudulating agent, antioxydant agent, antibacterial agent or antisense agent.

In one embodiment, the antiviral agent is chosen from a viral serine protease inhibitor, viral polymerase inhibitor and viral helicase inhibitor.

In one embodiment, the antiviral agent is chosen from interferon α and ribavirin.

In one embodiment, the additional agent is chosen from silybum marianum, interleukine-12, amantadine, ribozyme, thymosin, N-acetyl cysteine or cyclosporin.

In one embodiment, the compound of formula III and the additional agent are administered sequentially.

In one embodiment, the compound of formula III and the additional agent are administered simultaneously.

In one embodiment, there is provided a method for inhibiting or reducing the activity of viral polymerase in a host comprising administering a therapeutically effective amount of a compound of formula (III).

In one embodiment, the method for inhibiting or reducing the activity of viral polymerase in a host comprising administering a therapeutically effective amount of a compound of formula (III) is further comprising one or more viral polymerase inhibitor.

In further embodiments;

The viral polymerase is a Flaviviridae viral polymerase. The viral polymerase is a RNA-dependant RNA-polymerase. The viral polymerase is HCV polymerase.

In one embodiment, the invention provides a method for inhibiting or reducing the activity of viral helicase in a host comprising administering a therapeutically effective amount of a compound having the formula III.

In one embodiment, the invention provides a method for inhibiting or reducing the activity of viral helicase in a host comprising administering a therapeutically effective amount of a compound chosen from:

-   Compound #14     3-(4-Chloro-2,5-dimethyl-benzenesulfonylamino)-5-(4-chloro-phenyl)-thiophene-2-carboxylic     acid -   Compound #19     3-(4-Chloro-2,5-dimethyl-benzenesulfonylamino)-5-(4-isobutyl-phenyl)-thiophene-2-carboxylic     acid -   Compound #223     3-(4-Bromo-2-fluorobenzenesulfo-nylamino)-5-(4-isobutylphenyl)-thiophene-2-carboxylic     acid -   Compound #224     3-(4-Bromo-2-methylbenzenesulfo-nylamino)-5-(4-isobutylphenyl)-thiophene-2-carboxylic     acid -   Compound #225 5-(4-Isobutylphenyl     3-(3-methoxy-benzenesulfonyl-amino)-thiophene-2-carboxylic acid -   Compound #581     5-(4-Isobutyl-phenyl)-3-[5-(5-trifluoromethyl-isoxazol-3-yl)-thiophene-2-sulfonylamino]thiophene-2-carboxylic     acid -   Compound #227     3-[2,5-Bis-(2,2,2-trifluoroethoxy)-benzenesulfonylamino]-5-(4-isobutyl-phenyl)-thiophene-2-carboxylic     acid -   Compound #228     3-(2-Chloro-4-cyanobenzenesulfonylamino)-5-(4-isobutylphenyl)-thiophene-2-carboxylic     acid -   Compound #582     5-(4-Isobutyl-phenyl)-3-(2,3,4-trifluoro-benzenesulfonylamino)-thiophene-2-carboxylic     acid     or pharmaceutically acceptable salts thereof.     In further embodiments;     The viral helicase is a flaviviridea helicase.     The viral helicase is HCV helicase.

In a further embodiment, there is provided the use of a compound having the formula III for inhibiting or reducing the activity of viral polymerase in a host.

In still a further embodiment, there is provided the use of a compound having the formula III for inhibiting or reducing the activity of viral polymerase in a host, further comprising one or more viral polymerase inhibitor.

In further embodiments;

The viral polymerase is Flaviviridae viral polymerase. The viral polymerase is RNA-dependant RNA-polymerase. The viral polymerase is HCV polymerase.

In one embodiment, the invention provides the use of a compound having the formula III for inhibiting or reducing the activity of viral helicase in a host.

In one embodiment, the invention provides the use of a compound chosen from:

-   Compound #14     3-(4-Chloro-2,5-dimethyl-benzenesulfonylamino)-5-(4-chloro-phenyl)-thiophene-2-carboxylic     acid -   Compound #19     3-(4-Chloro-2,5-dimethyl-benzenesulfonylamino)-5-(4-isobutyl-phenyl)-thiophene-2-carboxylic     acid -   Compound #223     3-(4-Bromo-2-fluorobenzenesulfo-nylamino)-5-(4-isobutylphenyl)-thiophene-2-carboxylic     acid -   Compound #224     3-(4-Bromo-2-methylbenzenesulfo-nylamino)-5-(4-isobutylphenyl)-thiophene-2-carboxylic     acid -   Compound #225 5-(4-Isobutylphenyl     3-(3-methoxy-benzenesulfonyl-amino)-thiophene-2-carboxylic acid -   Compound #581     5-(4-Isobutyl-phenyl)-3-[5-(5-trifluoromethyl-isoxazol-3-yl)-thiophene-2-sulfonylamino]thiophene-2-carboxylic     acid -   Compound #227     3-[2,5-Bis-(2,2,2-trifluoroethoxy)-benzenesulfonylamino]-5-(4-isobutyl-phenyl)-thiophene-2-carboxylic     acid -   Compound #228     3-(2-Chloro-4-cyanobenzenesulfonylamino)-5-(4-isobutylphenyl)-thiophene-2-carboxylic     acid -   Compound #582     5-(4-Isobutyl-phenyl)-3-(2,3,4-trifluoro-benzenesulfonylamino)-thiophene-2-carboxylic     acid     or pharmaceutically acceptable salts thereof for inhibiting or     reducing the activity of viral helicase in a host.

In one embodiment, the invention provides the use of a compound having the formula III for inhibiting or reducing the activity of viral helicase in a host further comprising one or more viral helicase inhibitor.

In further embodiments;

The viral helicase is Flaviviridae viral helicase. The viral helicase is HCV helicase.

In one embodiment, the present invention provides a combination comprising a compound having the formula III and one or more additional agent chosen from viral serine protease inhibitor, viral polymerase inhibitor and viral helicase inhibitor, immunomudulating agent, antioxydant agent, antibacterial agent or antisense agent.

In a further embodiment, the additional agent is chosen from silybum marianum, interleukine-12, amantadine, ribozyme, thymosin, N-acetyl cysteine, cyclosporin, interferon α and ribavirin.

In further embodiments;

The compound of formula (III) and the additional agent are administered sequentially. The compound of formula (III) and the additional agent are administered simultaneously.

In still a further embodiment, the present invention provides a process for preparing a compound of formula A:

said process comprising the steps of adding:

-   -   an enol ether;     -   an hydride donating agent; and     -   an organic carboxylic acid;         to a compound of formula B:

wherein; Y¹ is chosen from a bond, C₁₋₆ alkyl, C₂₋₆ alkenyl or C₂₋₆ alkynyl; Y is chosen from COOR₁₆, COCOOR₅, P(O)OR_(a)OR_(b), S(O)OR₅, S(O)₂OR₅, tetrazole, CON(R₉)CH(R₅)COOR₅, CONR₁₀R₁₁, CON(R₉)—SO₂—R₅, CONR₉OH or halogen, wherein R₉, R₅, R₁₀ and R₁₁ are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or R₁₀ and R₁₁ are taken together with the nitrogen to form a 3 to 10 membered heterocycle; R_(a) and R_(b) are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl and C₆₋₁₈ aralkyl; or R_(a) and R_(b) are taken together with the oxygens to form a 5 to 10 membered heterocycle; R₁₆ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl and C₆₋₁₈ aralkyl; R₁ is chosen from C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl or halogen; R₂ is chosen from C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈ aralkyl; R₃ is chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl or C₆₋₁₈ aralkyl; Z is chosen from H, halogen, C₁₋₆ alkyl.

It will be appreciated by those skilled in the art that the compounds of formula (I) or (Ia) can contain a chiral centre on the general formula (I). The compounds of formula (I) or (Ia) thus exist in the form of two different optical isomers (i.e. (+) or (−) enantiomers). All such enantiomers and mixtures thereof including racemic mixtures are included within the scope of the invention. The single optical isomer or enantiomer can be obtained by method well known in the art, such as chiral HPLC, enzymatic resolution and chiral auxiliary.

In accordance with the present invention, the compounds of formula (I) or (Ia) include:

-   Compound 1     3-[(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYL)-(3-IODO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 2     3-[(3-BENZOFURAN-2-YL-BENZYL)-(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 3     3-(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 4     3-{(2,4-DICHLORO-BENZOYL)-[5-(3-TRIFLUOROMETHYL-PHENYL)-FURAN-2-YLMETHYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 5     3-[(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 6     5-(4-FLUORO-PHENYL)-3-(TOLUENE-4-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 7     3-(2,4-DICHLORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 8     3-(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYLAMINO)-5-(4-FLUORO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 9     3-[(2,4-DICHLORO-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 10     5-TERT-BUTYL-3-(4-CHLORO-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 11     4-(TOLUENE-4-SULFONYLAMINO)-[2,3′]BITHIOPHENYL-5-CARBOXYLIC ACID -   Compound 12     3-[(5-BENZOFURAN-2-YL-THIOPHEN-2-YLMETHYL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 13     5-PHENYL-3-(TOLUENE-4-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 14     3-(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYLAMINO)-5-(4-CHLORO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 15     5-PHENYL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 16     5-PHENYL-3-(TOLUENE-3-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 17 3-BENZENESULFONYLAMINO-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 18     3-(4-CHLORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 19     3-(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYLAMINO)-5-(4-ISOBUTYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 20     5-TERT-BUTYL-3-(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 21     3-(2,5-DIMETHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 22     3-(4-METHOXY-2,3,6-TRIMETHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 23     5-PHENYL-3-(THIOPHENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 24     4-(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYLAMINO)-[2,3′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 25     5-(3,5-BIS-TRIFLUOROMETHYL-PHENYL)-3-(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 26     8-CHLORO-3-(4-CHLORO-2,5-DIMETHYL-BENZENESULFONYLAMINO)-4H-1,5-DITHIA-CYCLOPENTA[A]NAPHTHALENE-2-CARBOXYLIC     ACID -   Compound 27     3-(2,4-DIFLUORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 28     3-[3-(2,6-DICHLORO-PYRIDIN-4-YL)-UREIDO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 29     3-(2-CHLORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 30     3-(2-FLUORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 31     5-PHENYL-3-(2-TRIFLUOROMETHOXY-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 32     3-(4-TERT-BUTYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 33     3-(4-CHLORO-PHENOXYCARBONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 34     3-(3,4-DICHLORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 35     5-PHENYL-3-(2-TRIFLUOROMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 36     3-(5-BROMO-6-CHLORO-PYRIDINE-3-SULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 37     3-(5-CHLORO-THIOPHENE-2-SULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 38     3-(5-CHLORO-3-METHYL-BENZO[B]THIOPHENE-2-SULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 39     3-(4-BROMO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 40     3-(3-CHLORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 41     3-(5-CHLORO-1,3-DIMETHYL-1H-PYRAZOLE-4-SULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 42     3-(3-BROMO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 43     3-(4-ISOPROPYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 44     3-(2,6-DICHLORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 45     3-(2-NITRO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 46     5-PHENYL-3-(5-[1,2,3]THIADIAZOL-4-YL-THIOPHENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 47     5-PHENYL-3-(PYRIDINE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 48     3-(2,4-DICHLORO-BENZYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 49     3-(3-FLUORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 50     5-PHENYL-3-(3-TRIFLUOROMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 51     3-(2-CARBOXY-BENZOYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID     METHYL ESTER -   Compound 52     5-PHENYL-3-(4-TRIFLUOROMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 53     3-(2,5-DIFLUORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 54     3-(2-CYANO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 55     3-(2,5-DICHLORO-THIOPHENE-3-SULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 56     4-(TOLUENE-2-SULFONYLAMINO)-[2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID -   Compound 57     5′-CHLORO-4-(TOLUENE-2-SULFONYLAMINO)-[2,2′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 58     5-(2,4-DICHLORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 59     5-(4-NITRO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 60     3-(TOLUENE-2-SULFONYLAMINO)-5-(4-TRIFLUOROMETHOXY-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 61     5-QUINOLIN-8-YL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 62     5-PHENYL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 63     5-(3-NITRO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 64     3-(TOLUENE-2-SULFONYLAMINO)-5-M-TOLYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 65     5-(3-CHLORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 66     5-(4-FLUORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 67     5-(3-FLUORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 68     5-(4-CHLORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 69     5-(3,5-DIFLUORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 70     5-(3,4-DIFLUORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 71     3-(TOLUENE-2-SULFONYLAMINO)-5-VINYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 72     3-(4-CHLORO-BENZOYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 73     3-[(4-CHLORO-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 74     5-PHENYL-3-[(THIOPHENE-2-CARBONYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 75     3-[METHYL-(THIOPHENE-2-CARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 76     3-(2-BROMO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 77     3-(2,4-DIFLUORO-BENZOYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 78     3-[(2,4-DIFLUORO-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 79     3-(TOLUENE-2-SULFONYLAMINO)-5-TRIMETHYLSILANYLETHYNYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 80     5-ETHYNYL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 81     3-(TOLUENE-2-SULFONYLAMINO)-5-(3-TRIFLUOROMETHOXY-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 82     5-BENZOYL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 83     5-(4-CYANO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 84     5-(3-CHLORO-4-FLUORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 85     5-(3,4-DICHLORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 86     5-PYRIDIN-4-YL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 87     5-PYRIDIN-3-YL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 88     3-(TOLUENE-2-SULFONYLAMINO)-5-(4-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 89     5-(4-METHANESULFONYL-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 90     5-(3-ACETYLAMINO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 91     5-(3-CHLORO-4-FLUORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 92     3-(4-METHYL-BENZOYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 93     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 94     3-(3,5-DIMETHYL-ISOXAZOLE-4-SULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 95     3-[(2-CHLORO-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 96     3-(2-METHYL-BENZOYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 97     3-[METHYL-(2-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 98     5-PHENYL-3-(5-TRIFLUOROMETHYL-PYRIDINE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 99     5-PHENYLETHYNYL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 100     3-(2,5-DIMETHYL-BENZENESULFONYLAMINO)-5-(4-NITRO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 101     5-(2-FLUORO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 102     5-(2-CYANO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 103     5-(2-ETHOXYCARBONYL-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 104     5-(2-METHOXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 105     3′-METHYL-4-(TOLUENE-2-SULFONYLAMINO)-[2,2′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 106     3-(TOLUENE-2-SULFONYLAMINO)-5-(2-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 107     3-(2,5-DIMETHYL-BENZENESULFONYLAMINO)-5-(4-FLUORO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 108     5-STYRYL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 109     3-(2,4-DIFLUORO-BENZENESULFONYLAMINO)-5-(4-NITRO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 110     3-(2,4-DIFLUORO-BENZENESULFONYLAMINO)-5-(4-FLUORO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 111     3-[[5-(3-CHLORO-4-FLUORO-PHENYL)-THIOPHEN-2-YLMETHYL]-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 112     3-[(4-OXO-1-PHENYL-1,3,8-TRIAZA-SPIRO[4.5]DECANE-8-CARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 113     3-{[4-(2-OXO-2,3-DIHYDRO-BENZOIMIDAZOL-1-YL)-PIPERIDINE-1-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 114     3-{[4-(4-NITRO-PHENYL)-PIPERAZINE-1-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 115 5-(2-CARB     OXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 116     5-(4-CHLORO-PHENYL)-3-(PYRIDINE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 117     5-(3-CYANO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 118     3-(2,5-DIMETHYL-BENZENESULFONYLAMINO)-5-P-TOLYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 119     3-(2,4-DIFLUORO-BENZENESULFONYLAMINO)-5-P-TOLYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 120     5-PHENETHYL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 121     5-(3-ETHOXYCARBONYL-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 122     5-(4-METHOXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 123     5-(3-METHOXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 124     5-(4′-BROMO-BIPHENYL-4-YL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 125     5-(4-HYDROXYMETHYL-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 126     5-FURAN-3-YL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 127     5-BENZOFURAN-2-YL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 128     5-PYRIDIN-2-YL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 129     5-(4-NITRO-PHENYL)-3-(PYRIDINE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 130     3-[(BENZOFURAN-2-CARBONYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 131     3-[(2,4-DIMETHYL-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 132     3-[[5-(2-CYANO-PHENYL)-THIOPHEN-2-YLMETHYL]-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 133     5-(4-FLUORO-PHENYL)-3-(PYRIDINE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 134     5-[2-(4-CHLORO-PHENYL)-VINYL]-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 135     3-BENZENESULFONYLAMINO-5-(4-FLUORO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 136     3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 137     5-PHENYL-3-(2-VINYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 138     3-(4-BROMO-2,5-DIFLUORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 139     3-(2-ACETYLAMINO-4-METHYL-THIAZOLE-5-SULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 140     3-(4-ACETYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 141     3-(4-FLUORO-2-TRIFLUOROMETHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 142     3-(2-METHOXY-4-METHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 143     3-(3,4-DIFLUORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 144 4-(2-CARB     OXY-5-PHENYL-THIOPHEN-3-YLSULFAMOYL)-5-(4-CHLORO-PHENYL)-2-METHYL-FURAN-3-CARBOXYLIC     ACID ETHYL ESTER -   Compound 145     3-(4-FLUORO-3-TRIFLUOROMETHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 146     3-(2-AMINO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 147     3-(3-NITRO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 148     3-(4-NITRO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 149     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 150     5-(3-CYANO-BENZYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 151     5-PHENYL-3-(2,4,6-TRIFLUORO-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 152     3-(4-METHOXY-2-NITRO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 153     5-PHENYL-3-(2,3,4-TRICHLORO-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 154     5-(2-CARBOXY-5-PHENYL-THIOPHEN-3-YLSULFAMOYL)-2-METHYL-FURAN-3-CARBOXYLIC     ACID METHYL ESTER -   Compound 155     4-(2-CARBOXY-5-PHENYL-THIOPHEN-3-YLSULFAMOYL)-2-METHYL-1,5-DIPHENYL-1H-PYRROLE-3-CARBOXYLIC     ACID ETHYL ESTER -   Compound 156     5-PHENYL-3-{[4-(3-TRIFLUOROMETHYL-PHENYL)-PIPERAZINE-1-CARBONYL]-AMIN}-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 157     3-{[4-(4-FLUORO-PHENYL)-PIPERAZINE-1-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 158     3-{[4-(2,6-DIMETHYL-PHENYL)-PIPERAZINE-1-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 159     3-{[4-(2-CHLORO-PHENYL)-PIPERAZINE-1-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 160     3-{[4-(3-CHLORO-PHENYL)-PIPERAZINE-1-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 161     4,4′-BIS-(TOLUENE-2-SULFONYLAMINO)-[2,2′]BITHIOPHENYL-5,5′-DICARBOXYLIC     ACID -   Compound 162     3-[ALLYL-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 163     5-(1-DIMETHYLSULFAMOYL-1H-PYRAZOL-4-YL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 164     5-(3-AMINO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 165     5-(4-AMINO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 166     5-(4-ACETYL-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 167     4-(2-CARBOXY-5-PHENYL-THIOPHEN-3-YLSULFAMOYL)-2,5-DIMETHYL-1H-PYRROLE-3-CARBOXYLIC     ACID ETHYL ESTER -   Compound 168     4-(2-CARBOXY-5-PHENYL-THIOPHEN-3-YLSULFAMOYL)-5-(4-CHLORO-PHENYL)-3-METHYL-1-PHENYL-1H-PYRROLE-2-CARBOXYLIC     ACID ETHYL ESTER -   Compound 169     3-(3,5-DICHLORO-4-HYDROXY-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 170     5-(1H-PYRAZOL-4-YL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 171     5-(3-HYDROXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 172     3-[METHYL-(3-METHYL-BUTYRYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 173     3-{[2-(4-FLUORO-PHENYL)-ACETYL]-METHYL-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 174     3-(4-PENTYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 175     3-(METHYL-PHENYLACETYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 176     3-[2,5-BIS-(2,2,2-TRIFLUORO-ETHOXY)-BENZENESULFONYLAMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 177     3-(4-METHYL-2-NITRO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 178     5-THIAZOL-2-YL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 179     5-PHENYL-3-[3-(3-PHENYL-PROPYL)-UREIDO]-THIOPHENE-2-CARBOXYLIC ACID -   Compound 180     3-[(3,4-DIHYDRO-1H-ISOQUINOLINE-2-CARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 181     3-{[4-(4-METHOXY-PHENYL)-PIPERAZINE-1-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 182     3-{[4-(6-METHYL-PYRIDIN-2-YL)-PIPERAZINE-1-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID HYDROCHLORIDE -   Compound 183     3-{[4-(4-CHLORO-BENZYL)-PIPERAZINE-1-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID HYDROCHLORIDE -   Compound 184     5-(5-METHYL-PYRIDIN-2-YL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 185     3-[ETHYL-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 186     3-[(3-CHLORO-THIOPHENE-2-CARBONYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 187     3-[(2-BROMO-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 188     3-[(4-BUTYL-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 189     3-(2-CHLOROMETHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 190     5-(4-HYDROXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 191     5-(5-CHLORO-PYRIDIN-2-YL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 192     5-(4-CHLORO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 193     5-(4-CYANO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 194     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-(4-NITRO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 195     5-(4-HYDROXYMETHYL-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 196     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-(3-NITRO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 197     5-(4-FLUORO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 198     5-(4-METHOXY-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 199     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-P-TOLYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 200     5-(4-AMINO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 201     3-[CYCLOPENTYL-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 202     5-BENZO[1,3]DIOXOL-5-YL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 203     3-[(2-HYDROXY-ETHYL)-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 204     3-[(2,4-DICHLORO-BENZOYL)-ISOBUTYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 205     3-[(2-METHOXY-4-METHYL-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 206     5-(3-CYANO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 207     5-(2-CHLORO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 208     3-[(2,4-DICHLORO-BENZOYL)-PHENYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 209     3-[4-(TRIFLUOROMETHYL-BENZOYL)METHYLAMINE]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 210     3-[(4-CHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 211     3-[ISOPROPYL-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 212     5-(3,5-DIFLUORO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 213     5-(3-FLUORO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 214     5-(2,4-DIFLUORO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 215     5-(4-HYDROXY-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 216     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-(4-TRIFLUOROMETHOXY-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 217     5-(2-HYDROXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 218     3-[(2-CHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 219     3-[(3,5-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 220     3-(4-BROMO-2-METHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 221 3-(5-CARB     OXY-4-CHLORO-2-FLUORO-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 222     5-PHENYL-3-(2,3,4-TRIFLUORO-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 223     3-(4-BROMO-2-FLUORO-BENZENESULFONYLAMINO)-5-(4-ISOBUTYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 224     3-(4-BROMO-2-METHYL-BENZENESULFONYLAMINO)-5-(4-ISOBUTYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 225     5-(4-ISOBUTYL-PHENYL)-3-(3-METHOXY-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 226     3-[(4-FLUORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 227     3-[2,5-BIS-(2,2,2-TRIFLUORO-ETHOXY)-BENZENESULFONYLAMINO]-5-(4-ISOBUTYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 228     3-(2-CHLORO-4-CYANO-BENZENESULFONYLAMINO)-5-(4-ISOBUTYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 229     5′-ACETYL-4-(TOLUENE-2-SULFONYLAMINO)-[2,2′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 230     5-BENZO[B]THIOPHEN-2-YL-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 231     5-(4-BUTYL-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 232     5-(4-ETHYL-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 233     3-[BENZYL-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 234     3-[(4-CHLORO-2-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 235     3-[(2,4-DIMETHYL-BENZENESULFONYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 236     5-(4-ACETYL-PHENYL)-3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 237     5-(4-ACETYL-PHENYL)-3-(TOLUENE-4-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 238     5-(4-ACETYL-PHENYL)-3-(4-CHLORO-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 239 5-(4-CARB     OXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID     TERT-BUTYL ESTER -   Compound 240     3-[(2,4-DIMETHYL-BENZENESULFONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 241     3-[ACETYL-(4-CHLORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 242 3-ETHANESULFONYLAMINO-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 243     3-[ISOPROPYL-(4-TRIFLUOROMETHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 244     3-[(2,4-DICHLORO-BENZOYL)-(3-METHYL-BUT-2-ENYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 245     3-[(2,6-DICHLORO-PYRIDINE-3-CARBONYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 246     3-[(6-CHLORO-PYRIDINE-3-CARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 247     3-[(4-TERT-BUTYL-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 248     5-(4-CARBOXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 249     5-(4-ETHOXY-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 250     3-[(2,6-DICHLORO-PYRIDINE-3-CARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 251     3-[(BENZO[B]THIOPHENE-2-CARBONYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 252     3-[METHYL-(NAPHTHALENE-2-CARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 253     3-[(3,4-DICHLORO-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 254     3-[(3,5-DICHLORO-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 255     3-[(4-BROMO-3-METHYL-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 256     3-[(3-CHLORO-BENZO[B]THIOPHENE-2-CARBONYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 257     3-[METHYL-(4-METHYL-3-NITRO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 258     5-(4-CARBAMOYL-PHENYL)-3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 259     5-(4-CARBAMOYL-PHENYL)-3-(TOLUENE-4-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 260     5-(1H-INDOL-5-YL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 261     3-[SEC-BUTYL-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 262     3-[(2,4-DIMETHYL-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 263     5-(4-AZIDO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 264     3-[(2,4-DICHLORO-BENZOYL)-(1-PHENYL-ETHYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 265     5-(4-CARBAMOYL-PHENYL)-3-(4-CHLORO-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 266     5-(2-FLUORO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 267     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-O-TOLYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 268     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-M-TOLYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 269     5-(3-CHLORO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 270     5-(3,4-DIFLUORO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 271     5-(3-AMINO-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 272     5-(3-ACETYL-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 273     5-(3-HYDROXY-PHENYL)-3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 274     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-(3-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 275     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-(4-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 276     3-[(3,4-DIMETHOXY-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 277     3-[METHYL-(2,4,6-TRIFLUORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 278     3-[(2,3-DIFLUORO-4-TRIFLUOROMETHYL-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 279     3-[(3-FLUORO-4-TRIFLUOROMETHYL-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 280     3-[(2,3-DIFLUORO-4-METHYL-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 281     3-[(2-FLUORO-4-TRIFLUOROMETHYL-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 282     5-(4-CARBAMOYL-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 283     5-(4-FLUORO-PHENYL)-3-[ISOPROPYL-(4-METHYL-BENZOYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 284     3-[(2-BROMO-4-CHLORO-BENZOYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 285     3-(2,6-DIMETHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 286     3-[METHYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 287     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID METHYL ESTER -   Compound 288     5-(4-CYANO-PHENYL)-3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 289     3-(4-CHLORO-BENZENESULFONYLAMINO)-5-(4-CYANO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 290     5-(4-CYANO-PHENYL)-3-(TOLUENE-4-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 291     5′-ACETYL-4-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-[2,2′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 292     5′-ACETYL-4-(2,6-DIMETHYL-BENZENESULFONYLAMINO)-[2,2′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 293     3-[METHYL-(4-METHYL-THIOPHENE-2-CARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 294     5-(3-CHLORO-PHENYL)-3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 295     5′-CYANO-4-(TOLUENE-2-SULFONYLAMINO)-[2,2′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 296     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-PYRIDIN-2-YL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 297     3-[(2,4-DICHLORO-THIOBENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 298     5-PHENYL-3-(2,4,6-TRIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 299     3-[(1-CARBOXY-ETHYL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 300     3-[(4-METHYL-BENZOYL)-(3-METHYL-BUT-2-ENYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 301     3-[(2-HYDROXY-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 302     3-[METHYL-(4-METHYL-BENZOYL)-AMINO]-5-PYRIDIN-3-YL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 303     5′-ACETYL-4-[METHYL-(4-METHYL-BENZOYL)-AMINO]-[2,2′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 304     3-[ISOPROPYL-(4-METHYL-BENZOYL)-AMINO]-5-(3-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 305     3-[ISOPROPYL-(4-METHYL-BENZOYL)-AMINO]-5-M-TOLYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 306     3-[(2-BROMO-4-CHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 307     3-[(4-CHLORO-2-FLUORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 308     3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-4-METHYL-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 309     3-[(2-BROMO-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 310     3-[(4-CHLORO-2-IODO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 311     3-[(4-CYANO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 312     3-[ALLYL-(4-METHYL-BENZOYL)-AMINO]-5-[4-(2-CARBOXY-VINYL)-PHENYL]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 313     3-[(4-CHLORO-2-HYDROXY-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 314     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-4-METHYL-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 315     5-TERT-BUTYL-3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 316     3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 317     3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 318     5-[4-(2-CARBOXY-ETHYL)-PHENYL]-3-[(4-METHYL-BENZOYL)-PROPYL-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 319     5-BENZOFURAN-2-YL-3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 320     3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-5-(4-HYDROXYMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 321     3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-5-(4-METHANESULFONYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 322     5-[4-(2-CARBOXY-VINYL)-PHENYL]-3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 323     3-[ALLYL-(4-METHYL-BENZOYL)-AMINO]-5-[3-(2-CARBOXY-VINYL)-PHENYL]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 324     3-[ISOPROPYL-(2,4,6-TRIMETHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 325     5-[3-(2-CARBOXY-ETHYL)-PHENYL]-3-[(4-METHYL-BENZOYL)-PROPYL-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 326     3-[(2-FLUORO-4-TRIFLUOROMETHYL-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 327     3-[TERT-BUTYL-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 328     3-[(2-AMINO-4-CHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 329     3-[(4-CHLORO-2-NITRO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 330     3-[(4-METHYL-BENZOYL)-(3-TRIFLUOROMETHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 331     3-[(3-FLUORO-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 332     5-(4-CARBOXY-PHENYL)-3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 333     3-[CYCLOPROPYL-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 334     3-[(3-TERT-BUTYL-BENZYL)-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 335     3-[(3-CHLORO-BENZYL)-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 336     3-[(2,4-DIFLUORO-BENZYL)-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 337     3-[(4-CHLORO-2,5-DIFLUORO-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 338     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-(2-METHYL-ALLYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 339     3-{ALLYL-[2-(4-CHLORO-PHENYL)-ACETYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 340     3-[BENZYL-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 341     3-[(4-CHLORO-BENZYL)-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 342     3-[(4-METHYL-BENZOYL)-(4-NITRO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 343     3-[(4-METHYL-BENZOYL)-(2-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 344     3-[(3-METHOXY-BENZYL)-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 345     3-[(2-CHLORO-BENZYL)-(4-METHYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 346     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-ISOBUTYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 347     3-[ALLYL-(2-NAPHTHALEN-2-YL-ACETYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 348     3-{ALLYL-[2-(2,4-DICHLORO-PHENYL)-ACETYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 349     3-{ALLYL-[2-(2-CHLORO-4-FLUORO-PHENYL)-ACETYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 350     3-{ALLYL-[2-(3,4-DICHLORO-PHENYL)-ACETYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 351     3-{ALLYL-[2-(2,4-DIFLUORO-PHENYL)-ACETYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 352     3-{ALLYL-[2-(4-TRIFLUOROMETHYL-PHENYL)-ACETYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 353     3-{ALLYL-[2-(2,6-DICHLORO-PHENYL)-ACETYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 354     3-[ALLYL-(2-M-TOLYL-ACETYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 355     5-(4-ACETYL-PHENYL)-3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 356     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-(4-FLUORO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 357     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-M-TOLYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 358     5′-ACETYL-4-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-[2,2′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 359     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-(3-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 360     4-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5′-METHYL-[2,2′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 361     3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-5-(4-METHOXY-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 362     3-(CYCLOHEXANECARBONYL-ISOPROPYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 363     3-[(2,4-DICHLORO-BENZOYL)-[1-(2,4-DICHLORO-BENZOYL)-PIPERIDIN-4-YL]-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 364     4-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(4-METHYL-BENZOYL)-AMINO]-PIPERIDINE-1-CARBOXYLIC     ACID TERT-BUTYL ESTER -   Compound 365     4-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-PIPERIDINE-1-CARBOXYLIC     ACID TERT-BUTYL ESTER -   Compound 366     3-[(4-METHYL-BENZOYL)-PIPERIDIN-4-YL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 367     5′-ACETYL-4-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-[2,3′]BITHIOPHENYL-5-CARBOXYLIC     ACID -   Compound 368     3-[(2,4-DICHLORO-BENZOYL)-PIPERIDIN-4-YL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 369     5-(4-METHANESULFONYLAMINO-PHENYL)-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 370     3-(4-FLUORO-2-METHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 371     3-[(3-METHYL-CYCLOHEXANECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 372     3-(4-CHLORO-2-METHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 373     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-(4-METHANESULFONYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 374     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-(4-METHANESULFINYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 375 5-(4-CARB     OXY-PHENYL)-3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 376     5-BENZOFURAN-2-YL-3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 377     3-[(2-ACETOXY-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 378     3-[ISOPROPYL-(2-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 379     3-[ISOPROPYL-(2-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 380     3-(CYCLOHEPTANECARBONYL-ISOPROPYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 381     3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-(3-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 382     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-METHYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 383     3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-(3-NITRO-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 384     3-[(3-CYCLOPENTYL-PROPIONYL)-METHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 385     3-(BUTYRYL-METHYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 386     3-(METHYL-PENT-4-ENOYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 387     3-[ISOPROPYL-(5-METHYL-3-OXO-3H-ISOINDOL-1-YL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 388     3-[METHYL-(3-METHYL-BUTYRYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 389     3-(METHYL-PENTANOYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 390     3-[METHYL-(4-METHYL-PENTANOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 391     3-(CYCLOPENTANECARBONYL-ETHYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 392     3-[(3-CYCLOPENTYL-PROPIONYL)-ETHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 393     3-(CYCLOBUTANECARBONYL-ETHYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 394     3-(BUT-2-ENOYL-ETHYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 395     3-[ISOPROPYL-(4-METHYL-2-VINYL-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 396     3-[ISOPROPYL-(4-METHYL-CYCLOHEX-1-ENECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 397     3-(ALLYL-HEXANOYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 398     3-(ALLYL-CYCLOBUTANECARBONYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 399     3-(ALLYL-PENTANOYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 400     3-[ALLYL-(4-METHYL-PENTANOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 401     3-[ALLYL-(2-CYCLOPENTYL-ACETYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 402     3-[(2-HYDROXY-4-METHYL-CYCLOHEXANECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 403     3-[(2,4-DICHLORO-BENZOYL)-(1-PHENYL-ETHYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 404     3-[(2,4-DICHLORO-BENZOYL)-(1-PHENYL-ETHYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 405     3-[ISOPROPYL-(3-METHYL-CYCLOPENT-3-ENECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 406     3-[(2-BENZYLOXY-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-(3-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 407     3-[(2,4-DIMETHYL-CYCLOHEXANECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 408     3-[ISOPROPYL-(3-METHYL-CYCLOPENTANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 409     3-[(2-HYDROXY-4-METHYL-CYCLOHEXANECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 410     5-PHENYL-3-[PROPIONYL-(4-TRIFLUOROMETHYL-BENZYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 411     3-[ISOBUTYRYL-(4-TRIFLUOROMETHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 412     3-[(3-METHYL-BUTYRYL)-(4-TRIFLUOROMETHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 413     3-[CYCLOPROPANECARBONYL-(4-TRIFLUOROMETHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 414     3-[CYCLOBUTANECARBONYL-(4-TRIFLUOROMETHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 415     3-[BUTYRYL-(4-TRIFLUOROMETHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 416     3-[(2-CYCLOPENTYL-ACETYL)-(4-TRIFLUOROMETHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 417     3-[(4-TERT-BUTYL-BENZYL)-PROPIONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 418     3-[(4-NITRO-BENZYL)-PROPIONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 419     3-[(3-METHYL-BUTYRYL)-(4-NITRO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 420     3-[CYCLOPROPANECARBONYL-(4-NITRO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 421     3-[(2-CHLORO-BENZYL)-ISOBUTYRYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 422     3-[(2-CHLORO-BENZYL)-(3-METHYL-BUTYRYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 423     3-[(2-CHLORO-BENZYL)-CYCLOPROPANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 424     3-[(ADAMANTANE-1-CARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 425     3-[(2-CHLORO-BENZYL)-CYCLOBUTANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 426     3-[ACETYL-(2-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 427     3-[(2-METHYL-BENZYL)-PROPIONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 428     3-[(2-HYDROXY-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-(3-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 429     3-[(1-ACETYL-PIPERIDIN-4-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 430     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-[4-(1H-TETRAZOL-5-YL)-PHENYL]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 431     3-[(2-CYANO-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 432     3-[CYCLOBUTANECARBONYL-(2-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 433     3-[BUTYRYL-(2-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 434     3-[ACETYL-(3-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 435     3-[CYCLOBUTANECARBONYL-(4-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 436     3-[CYCLOHEXANECARBONYL-(4-TRIFLUOROMETHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 437     3-[(4-TERT-BUTYL-BENZYL)-ISOBUTYRYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 438     3-[(4-TERT-BUTYL-BENZYL)-CYCLOPROPANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 439     3-[(4-TERT-BUTYL-BENZYL)-CYCLOBUTANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 440     3-[(4-TERT-BUTYL-BENZYL)-BUTYRYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 441     3-[(4-TERT-BUTYL-BENZYL)-CYCLOHEXANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 442     3-[(4-TERT-BUTYL-BENZYL)-(2-CYCLOPENTYL-ACETYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 443     3-[(2-CYCLOPENTYL-ACETYL)-(4-NITRO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 444     3-[(2-CHLORO-BENZYL)-CYCLOHEXANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 445     3-[(2-CYCLOPENTYL-ACETYL)-(3-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 446     3-[BUTYRYL-(3-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 447     3-[BUTYRYL-(2-CHLORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 448     3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-M-TOLYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 449     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-THIAZOL-2-YL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 450 3-(ACETYL-BENZYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 451     3-(BENZYL-PROPIONYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 452     3-[BENZYL-(2-METHOXY-ACETYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 453     3-[BENZYL-(3-METHYL-BUTYRYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 454     3-(BENZYL-CYCLOPROPANECARBONYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 455     3-[ACETYL-(4-CHLORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 456     3-[(4-CHLORO-BENZYL)-PROPIONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 457     3-[(4-CHLORO-BENZYL)-ISOBUTYRYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 458     3-[(4-CHLORO-BENZYL)-(3-METHYL-BUTYRYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 459     3-[(4-CHLORO-BENZYL)-CYCLOPROPANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 460     5-(4-ACETYL-PHENYL)-3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 461     3-[(4-CHLORO-BENZYL)-CYCLOBUTANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 462     3-[BUTYRYL-(4-CHLORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 463     3-[(4-CHLORO-BENZYL)-(2-CYCLOPENTYL-ACETYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 464     3-[ACETYL-(4-TRIFLUOROMETHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 465     3-[ISOBUTYRYL-(3-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 466     3-[CYCLOPROPANECARBONYL-(3-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 467     3-[(4-METHYL-BENZYL)-PROPIONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 468     3-[ISOBUTYRYL-(4-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 469     3-[CYCLOPROPANECARBONYL-(4-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 470     3-[BUTYRYL-(4-METHYL-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 471     3-[(3-METHOXY-BENZYL)-PROPIONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 472     3-[(3-METHOXY-BENZYL)-(3-METHYL-BUTYRYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 473     3-[CYCLOBUTANECARBONYL-(3-METHOXY-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 474     3-[(2-CARBAMOYL-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 475     3-[BUTYRYL-(3-METHOXY-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 476     3-[ACETYL-(3-CHLORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 477     3-[(3-CHLORO-BENZYL)-PROPIONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 478     3-[(3-CHLORO-BENZYL)-(2-METHOXY-ACETYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 479     3-[(3-CHLORO-BENZYL)-(3-METHYL-BUTYRYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 480     3-[(3-CHLORO-BENZYL)-CYCLOPROPANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 481     3-[(3-CHLORO-BENZYL)-CYCLOBUTANECARBONYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 482     3-[BUTYRYL-(3-CHLORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 483     3-[ACETYL-(2,4-DIFLUORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 484     3-[(2,4-DIFLUORO-BENZYL)-(2-METHOXY-ACETYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 485     3-[(2,4-DIFLUORO-BENZYL)-ISOBUTYRYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 486     3-[(2,4-DIFLUORO-BENZYL)-(3-METHYL-BUTYRYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 487     3-[BENZYL-(2-CYCLOPENTYL-ACETYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 488     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-(1H-INDOL-5-YL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 489     3-(BENZYL-CYCLOBUTANECARBONYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 490     3-[CYCLOHEXANECARBONYL-(2,4-DIFLUORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 491     3-{ALLYL-[2-(4-METHOXY-PHENYL)-ACETYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 492     3-(ETHYL-HEXANOYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 493 3-(BUTYRYL-ETHYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 494     3-[ETHYL-(4-METHYL-PENTANOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 495     3-[CYCLOBUTANECARBONYL-(2,4-DIFLUORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 496     3-[BUTYRYL-(2,4-DIFLUORO-BENZYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 497     3-(CYCLOPENTANECARBONYL-METHYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 498     3-(CYCLOHEXANECARBONYL-METHYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 499     3-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-PYRROLIDINE-1-CARBOXYLIC     ACID TERT-BUTYL ESTER -   Compound 500     3-[(1,4-DIMETHYL-CYCLOHEXANECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 501     5-(4-ETHYL-PHENYL)-3-[(2-HYDROXY-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 502     3-[(2-HYDROXY-4-METHYL-BENZOYL)-ISOPROPYL-AMINO]-5-M-TOLYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 503     3-[(2,4-DICHLORO-BENZOYL)-PYRROLIDIN-3-YL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 504     4-{5-CARBOXY-4-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-THIOPHEN-2-YL}-3,6-DIHYDRO-2H-PYRIDINE-1-CARBOXYLIC     ACID BENZYL ESTER -   Compound 505     3-{[2-(HYDROXYIMINO-METHYL)-4-METHYL-BENZOYL]-ISOPROPYL-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 506     3-[(1-CARBAMIMIDOYL-PIPERIDIN-4-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 507     4-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-AZEPANE-1-CARBOXYLIC     ACID TERT-BUTYL ESTER -   Compound 508     4-{[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-METHYL}-PIPERIDINE-1-CARBOXYLIC     ACID BENZYL ESTER -   Compound 509     3-[AZEPAN-4-YL-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 510     3-[(4-METHYL-CYCLOHEXANECARBONYL)-PIPERIDIN-4-YL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID LITHIUM SALT -   Compound 511     3-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-PIPERIDINE-1-CARBOXYLIC     ACID TERT-BUTYL ESTER -   Compound 512     3-[(4-BENZYLOXYCARBONYLAMINO-CYCLOHEXYL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 513     3-[ISOPROPYL-(4-METHYL-2-OXO-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 514     3-[(2,4-DICHLORO-BENZOYL)-PIPERIDIN-3-YL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID; COMPOUND WITH GENERIC INORGANIC NEUTRAL COMPONENT -   Compound 515     3-[(4-BENZYLOXYCARBONYLAMINO-CYCLOHEXYL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 516     3-[(2-BENZYLOXY-1-METHYL-ETHYL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 517     3-[(2,2-DIMETHYL-[1,3]DIOXAN-5-YL)-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 518     3-[(2,4-DICHLORO-BENZOYL)-(2-HYDROXY-1-HYDROXYMETHYL-ETHYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 519     3-[(2,4-DICHLORO-BENZOYL)-PIPERIDIN-4-YLMETHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 520     3-[(2-CHLORO-BENZOYL)-PIPERIDIN-4-YLMETHYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 521     3-[(4,6-DICHLORO-1H-INDOLE-2-CARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 522     3-[(2,4-DICHLORO-BENZOYL)-(2-HYDROXY-1-METHYL-ETHYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 523     4-{1-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-ETHYL}-PIPERIDINE-1-CARBOXYLIC     ACID BENZYL ESTER -   Compound 524     4-{5-CARBOXY-4-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-THIOPHEN-2-YL}-3,6-DIHYDRO-2H-PYRIDINE-1-CARBOXYLIC     ACID BENZYL ESTER -   Compound 525     3-[(4-METHYL-CYCLOHEXANECARBONYL)-PYRIDIN-4-YL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 526     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-5-PIPERIDIN-4-YL-THIOPHENE-2-CARBOXYLIC     ACID; COMPOUND WITH TRIFLUORO-ACETIC ACID -   Compound 527     3-[ISOPROPYL-(4-PROPYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 528     4-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-CYCLOHEXYL-AMMONIUM;     TRIFLUORO-ACETATE -   Compound 529     3-[(2,4-DICHLORO-BENZOYL)-(1-PIPERIDIN-4-YL-ETHYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID; COMPOUND WITH TRIFLUORO-ACETIC ACID -   Compound 530     3-[(CYCLOHEX-3-ENECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 531     3-[(4-ETHYL-CYCLOHEXANECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 532     3-[(4-CHLORO-CYCLOHEXANECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 533     4-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-3-METHYL-PIPERIDINE-1-CARBOXYLIC     ACID BENZYL ESTER -   Compound 534     3-[(2,4-DICHLORO-BENZOYL)-(2-METHOXY-CYCLOHEXYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 535     3-[(2,4-DICHLORO-BENZOYL)-(2,2-DIMETHYL-[1,3]DIOXAN-5-YL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 536     3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-(1-METHYL-PIPERIDIN-4-YL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 537     3-[(2,4-DICHLORO-BENZOYL)-(3-METHYL-PIPERIDIN-4-YL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID; COMPOUND WITH TRIFLUORO-ACETIC ACID -   Compound 538     3-[(2,4-DICHLORO-BENZOYL)-(2-HYDROXY-CYCLOHEXYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID Compound 539     4-{[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(4-METHYLCYCLOHEXANE     CARBONYL)-AMINO]-METHYL}-PIPERIDINE-1-CARBOXYLIC ACID BENZYL ESTER -   Compound 540     3-[((1R,2S,4R)-2-HYDROXY-4-METHYL-CYCLOHEXANECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 541     3-{ISOPROPYL-[1-(4-METHOXY-2,3,6-TRIMETHYL-BENZENESULFONYL)-5-METHYL-1,2,3,6-TETRAHYDRO-PYRIDINE-2-CARBONYL]-AMINO}-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 542     3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-4-FLUORO-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 543     3-[(2,4-DICHLORO-BENZOYL)-(1-METHYL-PIPERIDIN-4-YL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 544     4-{[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(4-METHYLCYCLOHEXANE     CARBONYL)-AMINO]-METHYL}-PIPERIDINIUM; TRIFLUORO-ACETATE -   Compound 545     3-[(2-TERT-BUTOXYCARBONYLAMINO-1-METHYL-ETHYL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 546     2-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-PROPYL-AMINE     TRIFLUOROACETIC ACID SALT -   Compound 547 3-[(3-CARB     OXY-CYCLOPENTYL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 548     3-[(3-CARBOXY-CYCLOPENTYL)-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 549     2-[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-CYCLOHEXYL-AMMONIUM     CHLORIDE -   Compound 550     3-(BENZOYL-METHYL-AMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 551     {[5-PHENYL-3-(TOLUENE-4-SULFONYLAMINO)-THIOPHENE-2-CARBONYL]-AMINO}-ACETIC     ACID -   Compound 552     5-BROMO-3-(TOLUENE-2-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID -   Compound 553     3-[CYCLOHEXYL-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 554     3-[[1,3]DIOXAN-5-YL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 555     3-[[2-(TERT-BUTYL-DIMETHYL-SILANYLOXY)-1-METHYL-2-PHENYL-ETHYL]-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 556     3-[[2-(TERT-BUTYL-DIMETHYL-SILANYLOXY)-1-METHYL-2-PHENYL-ETHYL]-(2,4-DICHLORO-BENZOYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 557     3-[(2,4-DICHLORO-BENZOYL)-(2-DIETHYLAMINO-THIAZOL-5-YLMETHYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 558     (5-{[(2-CARBOXY-5-PHENYL-THIOPHEN-3-YL)-(2,4-DICHLORO-BENZOYL)-AMINO]-METHYL}-THIAZOL-2-YL)-DIETHYL-AMMONIUM;     CHLORIDE -   Compound 559     5-(4-FLUORO-PHENYL)-3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 560     3-[((1S,2R,4S)-2-HYDROXY-4-METHYL-CYCLOHEXANECARBONYL)-ISOPROPYL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 561     3-[(2,4-DICHLORO-BENZOYL)-(2-METHOXY-1-METHYL-ETHYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 562     3-[(4S)-ISOPROPYL-(4-METHYL-CYCLOHEX-1-ENECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 566 3-METHYL-(4-METHYLBENZOYL)-AMINO)5-PHENYL     THIOPHENE-2-CARBOXYLIC ACID (2-HYDROXY-ETHYL)AMIDE -   Compound 567     5-PHENYL-3-(TOLUENE-4-SULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC ACID     CYCLOBUTYLAMIDE -   Compound 568     3-(2,4-DIMETHYL-BENZENESULFONYLAMINO)-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID AMIDE -   Compound 569     5-BROMO-3-[(2,4-DICHLORO-BENZOYL)-ISOPROPYL-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 570     5-(4-CHLORO-PHENYL)-3-[ISOPROPYL-(4-METHYL-CYCLOHEXANE-CARBONYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 571     5-(4′-CHLORO-BIPHENYL-4-YL)-3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 572     3-[(4-METHYL-CYCLOHEXANECARBONYL)-(TETRAHYDRO-PYRAN-4-YL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 573     3-[(4-METHYL-CYCLOHEXANECARBONYL)-(1-METHYL-PIPERIDIN-4-YL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 574     3-[(4-METHYL-CYCLOHEXANECARBONYL)-PIPERIDIN-4-YL-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 575     3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-(4-TRIFLUOROMETHYL-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 576     5-(4-CYANO-PHENYL)-3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 577     3-[ISOPROPYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-(4-METHOXY-PHENYL)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 578     3-[(2-METHOXY-1-METHYL-ETHYL)-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 579     3-[CYCLOHEXYL-(4-METHYL-CYCLOHEXANECARBONYL)-AMINO]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 581     5-(4-ISOBUTYL-PHENYL)-3-[5-(5-TRIFLUOROMETHYL-ISOXAZOL-3-YL)-THIOPHENE-2-SULFONYLAMINO]-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 582     5-(4-ISOBUTYL-PHENYL)-3-(2,3,4-TRIFLUORO-BENZENESULFONYLAMINO)-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 583     3-[(2,4-DICHLORO-PHENYL)-ISOPROPYL-CARBAMOYL]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID -   Compound 584     3-(METHYL-P-TOLYL-CARBAMOYL)-5-PHENYL-THIOPHENE-2-CARBOXYLIC ACID -   Compound 585     3-[(2,4-DICHLORO-PHENYL)-METHYL-CARBAMOYL]-5-PHENYL-THIOPHENE-2-CARBOXYLIC     ACID     or pharmaceutically acceptable salts thereof.

Preferably, the compounds of the present invention are provided in the form of a single enantiomer at least 95%, more preferrably at least 97% and most preferably at least 99% free of the corresponding enantiomer.

More preferably the compound of the present invention are in the form of the (+) enantiomer at least 95% free of the corresponding (−) enantiomer.

More preferably the compound of the present invention are in the form of the (+) enantiomer at least 97% free of the corresponding (−) enantiomer.

More preferably the compound of the present invention are in the form of the (+) enantiomer at least 99% free of the corresponding (−) enantiomer.

In a more preferred embodiment, the compound of the present invention are in the form of the (−) enantiomer at least 95% free of the corresponding (+) enantiomer.

Most preferably the compound of the present invention are in the form of the (−) enantiomer at least 97% free of the corresponding (+) enantiomer.

More preferably the compound of the present invention are in the form of the (−) enantiomer at least 99% free of the corresponding (+) enantiomer.

There is also provided a pharmaceutically acceptable salts of the present invention. By the term pharmaceutically acceptable salts of compounds of general formula (I) or (Ia) are meant those derived from pharmaceutically acceptable inorganic and organic acids and bases. Examples of suitable acids include hydrochloric, hydrobromic, sulphuric, nitric, perchloric, fumaric, maleic, phosphoric, glycollic, lactic, salicylic, succinic, toleune-p-sulphonic, tartaric, acetic, trifluoroacetic, citric, methanesulphonic, formic, benzoic, malonic, naphthalene-2-sulphonic and benzenesulphonic acids. Other acids such as oxalic, while not in themselves pharmaceutically acceptable, may be useful as intermediates in obtaining the compounds of the invention and their pharmaceutically acceptable acid addition salts.

Salts derived from appropriate bases include alkali metal (e.g. sodium), alkaline earth metal (e.g. magnesium), ammonium and NR₄+ (where R is C₁₋₄ alkyl) salts.

References hereinafter to a compound according to the invention includes compounds of the general formula (I) or (Ia) and their pharmaceutically acceptable salts.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.

As used in this application, the term “alkyl” represents a straight chain, branched chain or cyclic hydrocarbon moiety which may optionally be substituted by one or more of: halogen, nitro, nitroso, SO3R12, PO3RcRd, CONR13R14, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C6-12 aralkyl, C6-12 aryl, C1-6 alkyloxy, C2-6 alkenyloxy, C2-6 alkynyloxy, C6-12 aryloxy, C(O)C1-6 alkyl, C(O)C2-6 alkenyl, C(O)C2-6 alkynyl, C(O)C6-12 aryl, C(O)C6-12 aralkyl, C3-10 heterocycle, hydroxyl, NR13R14, C(O)OR12, cyano, azido, amidino or guanido;

wherein R12, Rc, Rd, R13 and R14 are each independently chosen from H, C1-12 alkyl, C2-12 alkenyl, C2-12 alkynyl, C6-14 aryl, C3-12 heterocycle, C3-18 heteroaralkyl, C6-18 aralkyl; or Rc and Rd are taken together with the oxygens to form a 5 to 10 membered heterocycle; or R13 and R14 are taken together with the nitrogen to form a 3 to 10 membered heterocycle. Useful examples of alkyls include isopropyl, ethyl, fluorohexyl or cyclopropyl. The term alkyl is also meant to include alkyls in which one or more hydrogen atoms is replaced by an oxygen, (e.g. a benzoyl) or an halogen, more preferably, the halogen is fluoro (e.g. CF3- or CF3CH2-).

The terms “alkenyl” and “alkynyl” represent an alkyl containing at least one unsaturated group (e.g. allyl, acetylene, ethylene).

The term “aryl” represents a carbocyclic moiety containing at least one benzenoid-type ring which may optionally be substituted by one or more of halogen, nitro, nitroso, SO3R12, PO3RcRd, CONR13R14, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C6-12 aralkyl, C6-12 aryl, C1-6 alkyloxy, C2-6 alkenyloxy, C2-6 alkynyloxy, C6-12 aryloxy, C(O)C1-6 alkyl, C(O)C2-6 alkenyl, C(O)C2-6 alkynyl, C(O)C6-12 aryl, C(O)C6-12 aralkyl, C3-10 heterocycle, hydroxyl, NR13R14, C(O)OR12, cyano, azido, amidino or guanido;

wherein R12, Rc, Rd, R13 and R14 are each independently chosen from H, C1-12 alkyl, C2-12 alkenyl, C2-12 alkynyl, C6-14 aryl, C3-12 heterocycle, C3-18 heteroaralkyl, C6-18 aralkyl; or Rc and Rd are taken together with the oxygens to form a 5 to 10 membered heterocycle; or R13 and R14 are taken together with the nitrogen to form a 3 to 10 membered heterocycle. Examples of aryl include phenyl and naphthyl.

The term “aralkyl” represents an aryl group attached to the adjacent atom by a C1-6alkyl, C1-6alkenyl, or C1-6alkynyl(e.g., benzyl).

The term “heterocycle” represents a saturated or unsaturated, cyclic moiety wherein said cyclic moeity is interrupted by at least one heteroatom, (e.g. oxygen, sulfur or nitrogen) which may optionally be substituted halogen, nitro, nitroso, SO3R12, PO3RcRd, CONR13R14, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C6-12 aralkyl, C6-12 aryl, C1-6 alkyloxy, C2-6 alkenyloxy, C2-6 alkynyloxy, C6-12 aryloxy, C(O)C1-6 alkyl, C(O)C2-6 alkenyl, C(O)C2-6 alkynyl, C(O)C6-12 aryl, C(O)C6-12 aralkyl, C3-10 heterocycle, hydroxyl, NR13R14, C(O)OR12, cyano, azido, amidino or guanido;

wherein R12, Rc, Rd, R13 and R14 are each independently chosen from H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, C₆₋₁₈ aralkyl; or Rc and Rd are taken together with the oxygens to form a 5 to 10 membered heterocycle; or R13 and R14 are taken together with the nitrogen to form a 3 to 10 membered heterocycle. It is understood that the term heterocyclic ring represents a mono or polycyclic (e.g., bicyclic) ring. Examples of heterocyclic rings include but are not limited to epoxide; furan; benzofuran; isobenzofuran; oxathiolane; dithiolane; dioxolane; pyrrole; pyrrolidine; imidazole; pyridine; pyrimidine; indole; piperidine; morpholine; thiophene and thiomorpholine.

The term “heteroaralkyl” represents an heterocycle group attached to the adjacent atom by a C₁₋₆ alkyl, C₁₋₆ alkenyl, or C₁₋₆ alkynyl.

When there is a sulfur atom present, the sulfur atom can be at different oxidation levels, ie. S, SO, or SO2. All such oxidation levels are within the scope of the present invention.

The term “independently” means that a substituent can be the same or different definition for each item.

As used in this application, the term “hydride donating agent” means a suitable ionic or covalent inorganic compound of hydrogen with another element (e.g. boron, sodium, lithium or aluminum) allowing the process to occur under the reaction conditions without causing adverse effect on the reagents or product. Useful examples of hydride donating agent include but are not limited to sodium borohydride (NaBH₄), sodium cyanoborohydride (NaCNBH₃), sodium triacetoxyborohydride (Na(OAc)₃BH) and borane-pyridine complexe (BH₃-Py). Alternatively, resin or polymer supported hydride donating agent on a may be used.

The term “organic carboxylic acid” include but is not limited to aliphatic acid (e.g. acetic, formic, trifluoroacetic), aromatic acid (e.g. benzoic and salicylic), dicarboxylic acid (e.g. oxalic and phthalic). It will be apparent to one of ordinary skill that resin supported organic carboxylic acid may also be used.

The term “enol ether” as used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Enol ethers may be obtained commercially or prepared by well-known methods. Non-limiting examples of preparation include alkylation or silylation of enolates obtained from carbonyl compounds such as aldehydes, ketones, esters.

It will be appreciated that the amount of a compound of the invention required for use in treatment will vary not only with the particular compound selected but also with the route of administration, the nature of the condition for which treatment is required and the age and condition of the patient and will be ultimately at the discretion of the attendant physician or veterinarian. In general however a suitable dose will be in the range of from about 0.1 to about 750 mg/kg of body weight per day, preferably in the range of 0.5 to 60 mg/kg/day, most preferably in the range of 1 to 20 mg/kg/day.

The desired dose may conveniently be presented in a single dose or as divided dose administered at appropriate intervals, for example as two, three, four or more doses per day.

The compound is conveniently administered in unit dosage form; for example containing 10 to 1500 mg, conveniently 20 to 1000 mg, most conveniently 50 to 700 mg of active ingredient per unit dosage form.

Ideally the active ingredient should be administered to achieve peak plasma concentrations of the active compound of from about 1 to about 75 μM, preferably about 2 to 50 μM, most preferably about 3 to about 30 μM. This may be achieved, for example, by the intravenous injection of a 0.1 to 5% solution of the active ingredient, optionally in saline, or orally administered as a bolus containing about 1 to about 500 mg of the active ingredient. Desirable blood levels may be maintained by a continuous infusion to provide about 0.01 to about 5.0 mg/kg/hour or by intermittent infusions containing about 0.4 to about 15 mg/kg of the active ingredient.

While it is possible that, for use in therapy, a compound of the invention may be administered as the raw chemical it is preferable to present the active ingredient as a pharmaceutical formulation. The invention thus further provides a pharmaceutical formulation comprising compounds of formula (I) or (Ia) or a pharmaceutically acceptable derivative thereof together with one or more pharmaceutically acceptable carriers therefor and, optionally, other therapeutic and/or prophylactic ingredients. The carrier(s) must be “acceptable” in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.

Pharmaceutical formulations include those suitable for oral, rectal, nasal, topical (including buccal and sub-lingual), transdermal, vaginal or parenteral (including intramuscular, sub-cutaneous and intravenous) administration or in a form suitable for administration by inhalation or insufflation. The formulations may, where appropriate, be conveniently presented in discrete dosage units and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing into association the active compound with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.

Pharmaceutical formulation suitable for oral administration may conveniently be presented as discrete units such as capsules, cachets or tablets each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution, a suspension or as an emulsion. The active ingredient may also be presented as a bolus, electuary or paste. Tablets and capsules for oral administration may contain conventional excipients such as binding agents, fillers, lubricants, disintegrants, or wetting agents. The tablets may be coated according to methods well known in the art. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, emulsifying agents, non-aqueous vehicles (which may include edible oils), or preservatives.

The compounds according to the invention may also be formulated for parenteral administration (e.g. by injection, for example bolus injection or continuous infusion) and may be presented in unit dose form in ampoules, pre-filled syringes, small volume infusion or in multi-dose containers with an added preservative. The compositions may take such forms as suspensions, solutions, or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing an/or dispersing agents. Alternatively, the active ingredient may be in powder form, obtained by aseptic isolation of sterile solid or by lyophilisation from solution, for constitution with a suitable vehicle, e.g. sterile, pyrogen-free water, before use.

For topical administration to the epidermis, the compounds according to the invention may be formulated as ointments, creams or lotions, or as a transdermal patch. Such transdermal patches may contain penetration enhancers such as linalool, carvacrol, thymol, citral, menthol and t-anethole. Ointments and creams may, for example, be formulated with an aqueous or oily base with the addition of suitable thickening and/or gelling agents. Lotions may be formulated with an aqueous or oily base and will in general also contain one or more emulsifying agents, stabilizing agents, dispersing agents, suspending agents, thickening agents, or colouring agents.

Formulations suitable for topical administration in the mouth include lozenges comprising active ingredient in a flavoured base, usually sucrose and acacia or tragacanth; pastilles comprising the active ingredient in an inert base such as gelatin and glycerin or sucrose and acacia; and mouthwashes comprising the active ingredient in a suitable liquid carrier.

Pharmaceutical formulations suitable for rectal administration wherein the carrier is a solid are most preferably presented as unit dose suppositories. Suitable carriers include cocoa butter and other materials commonly used in the art, and the suppositories may be conveniently formed by admixture of the active compound with the softened or melted carrier(s) followed by chilling and shaping in moulds.

Formulations suitable for vaginal administration may be presented as pessaries, tampons, creams, gels, pastes, foams or sprays containing in addition to the active ingredient such carriers as are known in the art to be appropriate.

For intra-nasal administration the compounds of the invention may be used as a liquid spray or dispersible powder or in the form of drops. Drops may be formulated with an aqueous or non-aqueous base also comprising one more dispersing agents, solubilising agents or suspending agents. Liquid sprays are conveniently delivered from pressurized packs.

For administration by inhalation the compounds according to the invention are conveniently delivered from an insufflator, nebulizer or a pressurized pack or other convenient means of delivering an aerosol spray. Pressurized packs may comprise a suitable propellant such as dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount.

Alternatively, for administration by inhalation or insufflation, the compounds according to the invention may take the form of a dry powder composition, for example a powder mix of the compound and a suitable powder base such as lactose or starch. The powder composition may be presented in unit dosage form in, for example, capsules or cartridges or e.g. gelatin or blister packs from which the powder may be administered with the aid of an inhalator or insufflator.

When desired the above described formulations adapted to give sustained release of the active ingredient may be employed.

The compounds of the invention may also be used in combination with other antiviral agents or in combination with any additional agents useful in therapy and may be administered sequentially or simultaneously.

In one aspect of the invention, the compounds of the invention may be employed together with at least one other antiviral agent chosen from protease inhibitors, polymerase inhibitors, and helicase inhibitors.

In another aspect of the invention, the compounds of the invention may be employed together with at least one other antiviral agent chosen from Interferon-α and Ribavirin.

The combinations referred to above may conveniently be presented for use in the form of a pharmaceutical formulation and thus pharmaceutical formulations comprising a combination as defined above together with a pharmaceutically acceptable carrier therefor comprise a further aspect of the invention.

The individual components of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations.

When the compounds of formula (I) or (Ia) or a pharmaceutically acceptable salts thereof is used in combination with a second therapeutic agent active against the same virus the dose of each compound may be either the same as or differ from that when the compound is used alone. Appropriate doses will be readily appreciated by those skilled in the art.

The following general schemes and examples are provided to illustrate various embodiments of the present invention and shall not be considered as limiting in scope.

Example 1 Preparation of 3-(2-Chloro-benzenesulfonylamino)-5-phenyl-thiophene-2-carboxylic acid, compound #29

STEP I 3-Amino-5-phenyl-thiophene-2-carboxylic acid

To a suspension of 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (5 g, 21.459 mmol) in a mixture of THF:MeOH:H₂O (3:2:1, 75 mL), 1N aqueous solution of LiOH.H₂O (64 mL, 64.378 mmol) was added. The reaction mixture was stirred at 85° C. (external temperature) for 4 h. Solvents were removed under reduced pressure and the residue was partitioned between water and ethyl acetate. The water layer was separated and acidified with 1N HCl solution and then ethyl acetate was added to it. The organic phase was separated, dried (Na₂SO₄) and concentrated to obtain 3-Amino-5-phenyl-thiophene-2-carboxylic acid (4.15 g, 88%) as a yellowish solid. ¹H NMR (DMSO-D₆, 400 MHz): 7.59 (d, 2H), 7.40 (m, 3H), 6.92 (s, 1H).

Step II

3-(2-Chloro-benzenesulfonylamino)-5-phenyl-thiophene-2-carboxylic acid

3-Amino-5-phenyl-thiophene-2-carboxylic acid (100 mg, 0.457 mmol) was taken in a mixture of dioxane and water (1:1, 25 mL) and then added sodium carbonate (242 mg, 2.285 mmol) and 1-chlorobenzenesulfonyl chloride (289 mg, 1.369 mmol). The reaction mixture was stirred at room temperature for 12 h. Half of the solvent was removed under reduced pressure and then diluted with water and ether in a separatory funnel. The ether layer was separated and the aqueous layer was acidified with 10% KHSO₄ solution. Ethyl acetate was added to the aqueous phase to dissolve the white precipitate. The ethyl acetate layer was separated, dried (Na₂SO₄) and concentrated to 5 mL. The white solid was filtered and then washed with cold ethyl acetate to obtain 3-(2-Chloro-benzenesulfonylamino)-5-phenyl-thiophene-2-carboxylic acid (125 mg, 69%). ¹H NMR (DMSO-D₆, 400 MHz): 10.51 (bs, 1H), 8.30 (d, 1H), 7.72-7.60 (m, 4H), 7.57 (m, 1H), 7.44 (m, 4H).

The following compounds were prepared in a similar manner as described in general scheme 1: Compound #3, Compound #5, Compound #7, Compound #13, Compound #15, Compound #16, Compound #17, Compound #18, Compound #21, Compound #22, Compound #23, Compound #29, Compound #30, Compound #34, Compound #37, Compound #38, Compound #39, Compound #40,

-   Compound #41, Compound #42, Compound #44, Compound #45, Compound     #46, Compound #49, Compound #50, Compound #52, Compound #53,     Compound #54, Compound #55, Compound #76, Compound #94

Example 2 3-(Toluene-2-sulfonylamino)-5-p-tolyl-thiophene-2-carboxylic acid, compound #62

Step I

3-(bis-(Toluene-2-sulfonyl)-amino)-thiophene-2-carboxylic acid methyl ester

To a cold (0° C.) stirred sodium hypochlorite (NaOCl, 10.8% commercial bleach, 124 mL, 180.00 mmol) solution was added o-thiocresol (2.23 g, 2.12 mL, 18.0 mmol). To this vigorous stirred solution was added conc. Sulfuric acid (caution! extremely exothermic, 92 g, 50 mL, 938 mmol) dropwise. The resultant yellow reaction mixture was stirred for 2 h at the same temperature, diluted with water (50 mL) and dichloro-methane 50 mL. The organic solution was separated, aqueous solution was extracted with CH₂Cl₂ (2×50 mL). The combined organic extracts were washed with water, brine and dried. Evaporation of the solvent under reduced pressure furnished the 2-methylsulfonyl chloride (3.13 g, 91.5% yield), which was used in the next step without purification. ¹H NMR (CDCl₃,300 MHz) 8.07 (td, J=7.3, 1.5 Hz, 1H), 7.61 (tt, J=7.5, 1.1 Hz, 1H), 7.44-7.40 (m, 2H), 2.80 (s, 3H).

To a stirred solution of the methyl 3-amino-thiophene-2-carboxylic acid (1.0 g, 6.36 mmol) and DMAP (776 mg, 6.36 mmol) in CH₂Cl₂ was sequentially added triethyl amine (1.61 g, 15.9 mmol, 2.5 eq) and o-toluenesulfonyl chloride (3.02 g, 15.9 mmol, 2.5 eq), stirred for 24 h. The reaction mixture was diluted with EtOAc (100 mL), washed with 1.2 N HCl (2×50 mL), 6 N HCl (40 mL), saturated NaHCO₃ solution, brine and dried. Evaporation of the solvent under reduced pressure yielded 3-(bis-(Toluene-2-sulfonyl)-amino)-thiophene-2-carboxylic acid methyl ester (2.78 g, 93.3%) as a solid. The crude product was used in the next step without purification. ¹H NMR (CDCl₃,300 MHz) 8.198 (dd, J=8.0, 1.2 Hz, 2H), 7.52 (d, J=5.3 Hz, 1H), 7.5 (dt, J=7.5 Hz, 1.1 Hz, 2H), 7.36 (t, J=7.5 Hz, 3H), 7.28 (d, J=7.6 Hz, 2H), 7.16 (d, J=5.3 Hz, 1H), 3.44 (s, 3H), 2.43 (s, 3H).

Step II

3-(Toluene-2-sulfonylamino)-thiophene-2-carboxylic acid

To a stirred mixture of 3-(bis-(Toluene-2-sulfonyl)-amino)-thiophene-2-carboxylic acid methyl ester (2.5 g, 5.35 mmol) in 1,4-dioxane/MeOH/water (3:1:1; 62.5 mL) was added aq. 1 N NaOH solution (16.05 mL, 16.05 mmol, 3.0 eq) and heated at 85° C. for 3.5 h and it was then cooled to rt. To the reaction mixture was added 1.2 N HCl (16.0 mL), extracted with CHCl₃ (3×30 mL), washed with brine and dried. Evaporation of the solvent gave 3-(Toluene-2-sulfonylamino)-thiophene-2-carboxylic acid (1.5 g, 99%) as a white solid. ¹H NMR (DMSO-d₆,300 MHz) 7.94 (dd, J=7.9 Hz, 1.3 Hz, 1H), 7.76 (d, J=5.5 Hz, 1H), 7.55 (dt, J=7.5 Hz, 1.3 Hz, 1H), 7.42-7.37 (m, 2H), 7.1 (d, J=5.5 Hz, 1H), 2.57 (s, 3H).

Step III

3-(Toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester

To a cold (−40° C.) mixture of 3-(Toluene-2-sulfonylamino)-thiophene-2-carboxylic acid (1.5 g, 5.05 mmol) in 1,4-dioxane/CHCl₃ (1:2, 12 mL) was bubbled 2-methyl-2-propene gas (15 mL) in a sealed tube. To this was added Conc. H₂SO₄ (0.070 mL, 1.3 mmol) and slowly warmed up to room temperature. The resultant reaction mixture was heated at 70° C. for 2.5 days in a sealed tube, cooled to −40° C., stopper was removed. The reaction mixture was slowly brought up to room temperature and stirred until the excess gas is released. The mixture was extracted with EtOAc, washed with aq. NaHCO₃ solution, brine and dried. Evaporation of the solvent and purification of the residue on silica gel using EtOAc/hexane (1:10) as an eluent furnished 3-(Toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester (1.31 g, 73.5% based on 90% conversion). ¹H NMR (CDCl₃,300 MHz) 9.89 (s, 1H), 8.01 (d, J=7.9 Hz, 1H), 7.43 (dt, J=7.5 Hz, 1.5 Hz, 1H), 7.3-7.25 (m, 3H), 7.2 (d, J=5.4 Hz, 1H), 2.69 (s, 3H), 1.56 (s, 9H).

Step IV

5-Bromo-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester

To a cold (−30° C.) stirred solution of diisopropylamine (1.345 g, 1.86 mL, 13.3 mmol, 3.6 eq) in THF (74.0 mL) was added n-BuLi (1.6 M in hexane, 7.63 mL, 12.21 mmol, 3.3 eq) dropwise and stirred for 20 min. To the cold (−78° C.) LDA solution was added a solution of 3-(Toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester (1.31 g, 3.7 mmol, 1.0 eq) in THF (20 mL) dropwise and the solution was stirred for 2 h at the same temperature. The resultant red colored solution was then quenched with 1,2-dibromotetrafluoroethane (5.77 g, 2.65 mL, 22.2 mmol, 6.0 eq, passed through K₂CO₃ prior to use) in one portion, stirred for 1 h before being added sat. NH₄Cl solution (15.0 mL). The reaction mixture was warmed up to rt, extracted with EtOAc, washed with brine and dried. Evaporation of the solvent and purification of the residue over silica gel column furnished 5-Bromo-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester (1.2 g, 75% yield). ¹H NMR (CDCl₃, 300 MHz) 9.72 (s, 1H), 8.0 (dd, J=7.8, 1.3 Hz, 1H), 7.47 (dt, J=7.5, 1.2 Hz, 1H), 7.35-7.30 (m, 2H), 7.24 (s, 1H), 2.68 (s, 3H), 1.53 (s, 9H).

Step V

3-(Toluene-2-sulfonylamino)-5-p-tolyl-thiophene-2-carboxylic acid tert-butyl ester

To the mixture of 4-methylbenzeneboronic acid (38.0 mg, 0.279 mmol) and 5-Bromo-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester (40 mg, 0.0925 mmol) in 5:1 mixture of toluene/MeOH (2.0 mL) was added a solution of Pd(PPh₃)₄ (12.0 mg, 0.01 mmol, 10 mol %) in toluene (1.0 mL) followed by aqueous 2M Na₂CO₃ solution (0.1 mL, 0.2 mmol). The resultant reaction mixture was heated at 70° C. for 16 h, cooled to room temperature, filtered off through MgSO₄ and washed with EtOAc. Evaporation of the solvent and purification of the residue over preparative TLC (1 mm, 60A° using ethyl acetate/hexane (1:10) as an eluent furnished 3-(Toluene-2-sulfonylamino)-5-p-tolyl-thiophene-2-carboxylic acid tert-butyl ester (36.0 mg, 81% yield). ¹H NMR (CDCl₃, 300 MHz) 9.94 (s, 1H), 8.05 (d, J=8.0 Hz, 1H), 7.44-7.25 (m, 6H), 7.18 (d, J=8.1 Hz, 2H), 2.71 (s, 3H), 2.36 (s, 3H), 1.56 (s, 9H).

Step VI

3-(Toluene-2-sulfonylamino)-5-p-tolyl-thiophene-2-carboxylic acid

To a stirred solution of 3-(Toluene-2-sulfonylamino)-5-p-tolyl-thiophene-2-carboxylic acid tert-butyl ester (36.0 mg, 0.081 mmol) in CH₂Cl₂ (1.0 mL) was added TFA (0.5 mL), stirred for 1 h at room temperature and diluted with hexane. Evaporation of the solvent under reduced pressure gave essentially the pure product as a solid. The product was purified by triturating with hexane/CH₂Cl₂ furnished 3-(Toluene-2-sulfonylamino)-5-p-tolyl-thiophene-2-carboxylic acid (28.0 mg, 89% yield). ¹H NMR (DMSO-d₆, 300 MHz) 10.21 (br s, 1H), 8.06 (d, J=7.9 Hz, 1H), 7.56-7.36 (m, 6H), 7.24 (d, J=7.9 Hz, 2H), 2.59 (s, 3H), 2.48 (s, 3H).

The following compounds were prepared in a similar manner as described in general scheme 2:

-   Compound #6, Compound #8, Compound #11, Compound #14, Compound #24,     Compound #56, Compound #57, Compound #58, Compound #59, Compound     #60, Compound #62, Compound #63, Compound #64, Compound #65,     Compound #66, Compound #67, Compound #68, Compound #69, Compound     #70, Compound #71, Compound #552, Compound #79, Compound #80,     Compound #81, Compound #83, Compound #84, Compound #85, Compound     #86, Compound #87, Compound #88, -   Compound #89, Compound #90, Compound #91

Example 3 3-(4-Choro-benzoylamino)-5-phenyl-thiophene-2-carboxylic acid compound #72

STEP I 3-(4-Chloro-benzoylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester

To mixture of methyl-3-amino-5-phenylthiophene-2-carboxylate (100 mg, 0.428 mmol) in anhydrous pyridine (4.3 ml) was added p-chlorobenzoyl chloride (71 μl, 0.556 mmol). The mixture was stirred for 3 hours at room temperature and concentrated. Purification chromatography (silica gel, hexane to hexane:ethyl acetate; 95:5) gave 145 mg (91% yield) of 3-(4-Chloro-benzoylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester. ¹H NMR (CDCl₃, 400 MHz) 8.54 (s, 1H), 7.99-7.96 (m, 2H), 7.73-7.71 (m, 2H), 7.52-7.50 (m, 2H), 7.46-7.39 (m, 3H), 3.95 (s, 3H).

Step II

3-(4-Chlorobenzoylamino)-5-phenyl-thiophene-2-carboxylic acid

To a mixture of 3-(4-Chloro-benzoylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester (30 mg, 0.081 mmol) in 1 ml of a 3:2:1 solution made with tetrahydrofuran, methanol and water respectively was added lithium hydroxide monohydrated (20 mg, 0.484 mmol). The mixture was stirred 30 minutes at 60° C., cooled to room temperature, diluted with water and washed with ether (2×). The collected aqueous layer was then acidified with KHSO₄ 20% to pH 3 and extracted with ethyl acetate (3×). The combined ethyl acetate layers were washed with brine, dried (Na₂SO₄) and concentrated. The resulting crude was taken in ethyl acetate and reextracted with NaOH 0.5 N (2×). The combined aqueous layers were then back-washed with ethyl acetate and acidified to pH3 with KHSO₄ 20% and back-extracted with ethyl acetate (2×). The combined organic layers were washed with brine and dried (Na₂SO₄). ¹H NMR (DMSO-d₆, 400 MHz) 8.35 (s, 1H), 8.02-7.99 (m, 2H), 7.71-7.68 (m, 2H), 7.56-7.53 (m, 2H), 7.43-7.39 (m, 2H), 7.35-7.31 (m, 1H).

The following compounds were prepared in a similar manner as described in example 3:

Compound #74, Compound #77, Compound #92, Compound #96; Example 4 3-(Benzoyl-methyl-amino)-5-phenyl-thiophene-2-carboxylic acid; compound #550

Step I

3-Methylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester

To a mixture of methyl-3-amino-5-phenylthiophene-2-carboxylate (200 mg, 0.855 mmol) in anhydrous N,N-dimethylformamide (4.6 ml) were added 4.2 ml (8.55 mmol) of 2M iodomethane solution in t-buthylmethylether. The mixture was stirred at 60° C. for 18 hours, concentrated and purified using biotage technics (silica gel, hexane to hexane:ethyl acetate; 95:5 containing few drops of triethylamine) to give 68 mg (32% yield) of 3-methylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester. ¹H NMR (CDCl₃, 400 MHz) 7.65-7.62 (m, 2H), 7.42-7.36 (m, 3H), 6.86 (broad s, 1H), 3.83 (s, 3H), 3.04 (d, 3H)

Step II

3-(Benzoyl-methyl-amino)-5-phenyl-thiophene-2-carboxylic acid methyl ester

This compound was prepared in a similar manner as for Example 3, Step I; 3-(Benzoyl-methyl-amino)-5-phenyl-thiophene-2-carboxylic acid methyl ester was obtained ¹H NMR (CDCl₃, 400 MHz) 7.60-7.49 (m, 2H), 7.47-7.35 (m, 5H), 7.28-7.20 (m, 3H), 7.11 (broad s, 1H), 3.83 (s, 3H), 3.44 (s, 3H)

Step III

3-(Benzoyl-methyl-amino)-5-phenyl-thiophene-2-carboxylic acid

This compound was prepared in a similar manner as in Example 3, step II; 3-(Benzoyl-methyl-amino)-5-phenyl-thiophene-2-carboxylic acid was obtained; ¹H NMR (CD₃OD, 400 MHz) 7.64-7.62 (m, 2H), 7.47 (s, 1H), 7.44-7.36 (m, 5H), 7.29-7.20 (m, 3H), 3.42 (s, 3H)

The following compounds were prepared in a similar manner as described in example 4:

-   Compound #9; Compound #73 Compound #75; Compound #75; Compound #78;     Compound #93; Compound #95

Example 5 {[5-Phenyl-3-(toluene-4-sulfonylamino)-thiophene-2-carbonyl]-amino}-acetic acid, compound #551

Step I

{[5-Phenyl-3-(toluene-4-sulfonylamino)-thiophene-2-carbonyl]-amino}-acetic acid methyl ester

To a mixture of 5-phenyl-3-(toluene-4-sulfonylamino)-thiophene-2-carboxylic acid (prepared according to example 2) (50 mg, 0.134 mmol) in anhydrous dimethylformamide (1.4 ml) were added HATU 152 mg, 0.402 mmol), glycine methyl ester hydrochloride (20 mg, 0.161 mmol) followed by collidine (124 μl, 0.938 mmol). The mixture was stirred at room temperature for 1 hour, concentrated and pre-absorbed on SiO₂. Purification chromatography (hexane to hexane:ethyl acetate; 6:4 to dichloromethane: methanol; 95:5) gave 47 mg of a mixture of {[5-Phenyl-3-(toluene-4-sulfonylamino)-thiophene-2-carbonyl]-amino}-acetic acid methyl ester and collidine. ¹H NMR (CDCl₃, 400 MHz) 7.76-7.73 (m, 2H), 7.61 (s, 1H), 7.57-7.54 (m, 2H), 7.42-7.36 (m, 3H), 7.24-7.22 (m, 2H), 6.19-6.17 (m, 1H), 4.14-4.12 (m, 2H), 3.79 (s, 3H), 2.35 (s, 3H).

Step II

{[5-Phenyl-3-(toluene-4-sulfonylamino)-thiophene-2-carbonyl]-amino}-acetic acid

Following the procedure described for example 3 (STEP II), 28 mg (88% yield) of {[5-phenyl-3-(toluene-4-sulfonylamino)-thiophene-2-carbonyl]-amino}-acetic acid were isolated from 33 mg (0.075 mmol) of the {[5-Phenyl-3-(toluene-4-sulfonylamino)-thiophene-2-carbonyl]-amino}-acetic acid methyl ester. ¹H NMR (CD₃OD, 400 MHz): 7.73-7.71 (m, 2H), 7.63-7.61 (m, 2H), 7.54 (s, 1H), 7.45-7.39 (m, 3H), 7.33-7.31 (m, 2H), 4.88 (s, 2H), 2.36 (s, 3H).

Example 6 3-(2,4-Dichloro-benzylamino)-5-phenyl-thiophene-2-carboxylic acid Compound #48

Step I

3-(2,4-Dichloro-benzylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester

Sodium hydride (60% dispersion in oil, 180 mg, 4.72 mmol) was added to an ice-cold solution of 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (1000 mg, 4.29 mmol) in 25 ml of dimethylformamide in an atmosphere of N₂. After 5 min, 2,4-dichloro-1-chloromethyl-benzene (755 mg, 3.86 mmol) was added to the solution and then the reaction mixture was stirred for 30 min at 0° C. and 30 min at room temperature. The mixture was partitioned between ether (20 mL) and water (20 mL) and the organic layer was separated. The aqueous phase was washed twice with ether (2×20 mL) and the combined ether layer was dried (MgSO₄) and concentrated. The residue obtained was then purified by precipitation. The crude product was taken in 25 ml of ethyl acetate, a yellow precipitate came out which was filtered to obtain 3-(2,4-Dichloro-benzylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester, 835 mg (55%). ¹H-NMR (DMSO, 400 MHz): 7.67 ppm (m, 2H, H_(aro)); 7.44-7.35 ppm (m, 6H, H_(aro)); 7.26 ppm (s, 1H, H_(aro)); 4.63 ppm (d, 2H, N—CH₂); 3.75 ppm (s, 3H, O—CH)

Step II

3-(2,4-Dichloro-benzylamino)-5-phenyl-thiophene-2-carboxylic acid

3-(2,4-Dichloro-benzylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester (70 mg, 0.18 mmol) was dissolved in a mixture of THF-MeOH—H₂O (3:2:1) (20 mL) and then 1080 ul of LiOH 1N was added to it. After 16 h of stirring at temperature of 100° C., solvents were removed and then partitioned between 10 ml of H₂O, 2 ml of KHSO₄ 5% and 10 ml of EtOAc. The organic layer was separated and the aqueous phase was washed twice with ethyl acetate (2×10 mL). The combined ethyl acetate layer was dried (MgSO4) and concentrated to obtain 43 mg (63%) of 3-(2,4-Dichloro-benzylamino)-5-phenyl-thiophene-2-carboxylic acid ¹H-NMR (DMSO, 400 MHz): 7.65 ppm (m, 3H, H_(aro)); 7.43-7.32 ppm (m, 5H, H_(aro)); 7.23 ppm (s, 1H, H_(aro)); 4.61 ppm (d, 2H, N—CH₂).

Example 7 3-{(2,4-Dichloro-benzoyl)-[5-(3-trifluoromethyl-phenyl)-furan-2-ylmethyl]-amino}-5-phenyl-thiophene-2-carboxylic acid, Compound #4

Step I

5-Phenyl-3-{[5-(3-trifluoromethyl-phenyl)-furan-2-ylmethyl]-amino}-thiophene-2-carboxylic acid methylester

To a stirred solution of 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (100 mg, 0.416 mmol) in dichloromethane (15 mL) were added 5-(trifluoromethyl-phenyl)-furan-2-carbaldehyde (100 mg, 0.429 mmol) and molecular sieves. The reaction mixture was stirred at room temperature overnight. The solution was filtered over celite and the filtrate was evaporated under reduced pressure. The residue was dissolved in anhydrous methanol (15 mL). and cooled to 0° C. in an ice bath. Sodium borohydride (18 mg, 1.1 eq.) was added. The reaction mixture was stirred at this temperature for 2 h. Saturated ammonium chloride (10 mL) was added and stirring was continued for an additional 15 min. at room temperature. Methanol was removed and the resulted mixture was extracted with dichloromethane (3×30 mL). The organic solution was washed with water, brine and was dried over sodium sulfate. Solvent was evaporated and the crude product was purified on silica gel using hexane:ethylacetate 9:1 as eluent to provide the desired product in 34% yield (65 mg). ¹HNMR (CDCl₃, 400 MHz): 7.80 (s, 1H), 7.73 (m, 1H), 7.55 (m, 2H), 7.41 (m, 2H), 7.33 (m, 3H), 6.93 (s, 1H), 6.48 (d, 1H), 6.24 (d, 1H), 4.43 (s, 2H), 3.76 (s, 3H).

Step II

3-{(2,4-Dichloro-benzoyl)-[5-(3-trifluoromethyl-phenyl)-furan-2-ylmethyl]-amino}-5-phenyl-thiophene-2-carboxylic acid methyl ester

To a stirred solution of 5-Phenyl-3-{[5-(3-trifluoromethyl-phenyl)-furan-2-ylmethyl]-amino}-thiophene-2-carboxylic acid methylester (65 mg 0.142 mmol) in dichloromethane (3 ml) and saturated NaHCO₃ solution (3 ml) was added a solution of 2,4-dichloro-benzoyl chloride (36 mg, 1.2 eq.) in dichloromethane (0.9 ml). The reaction mixture was stirred vigorously at room temperature for overnight. The organic phase was collected and the aqueous phase was extracted twice with methylene chloride (2×15 ml). The organic layers were combined, washed with water, brine and dried over anhydrous Na₂SO₄. Solvent was removed and residue was purified on silica gel using Hexane:EtOAc 9:1 as eluant to give the desired product in 78% yield (70 mg). The proton NMR indicated the presence of rotamers.

¹HNMR (CDCl₃, 400 MHz): 7.80 (s, 1H), 7.73 (m, 1H), 7.55 (m, 2H), 7.45 (m, 2H), 7.33 (m, 3H), 7.20 (m, 2H), 7.12 (m, 1H), 6.93 (s, 1H), 6.62 (d, 1H), 6.42 (d, 1H), 5.60 (bd, 1H), 4.70 (bd, 1H), 3.76 (s, 3H).

Step III

3-{(2,4-Dichloro-benzoyl)-[5-(3-trifluoromethyl-phenyl)-furan-2-ylmethyl]-amino}-5-phenyl-thiophene-2-carboxylic acid

3-{(2,4-Dichloro-benzoyl)-[5-(3-trifluoromethyl-phenyl)-furan-2-ylmethyl]-amino}-5-phenyl-thiophene-2-carboxylic acid methyl ester (62 mg, 0.098 mmol) was dissolved in THF (5 mL) and water (2 mL). A solution of lithium hydroxide (13 mg, 3 eq. in 2 mL of water) was added dropwise. After first few drop, a pink color appeared and disappeared. Mixture was stirred for 5 hrs and acidified with 1N HCl-solution. The product was extracted into ethyl acetate, washed once with water, dried over magnesium sulfate. Solvent was evaporated and the residue was purified on silica gel (Bond-Elute 2 g). The product was elute with a 20 mL gradient of Hexane:EtOAc 9:1.4:1, 7:3, 3:2, 1:1, 2:3 and EtOAc to give the desired product in 76% yield (46 mg).

¹HNMR (CD₃OD, 400 MHz): 7.90 (s, 1H), 7.83 (m, 1H), 7.55 (m, 2H), 7.40-7.20 (m, 8H), 7.10 (s, 1H), 6.82 (d, 1H), 6.42 (d, 1H), 5.60 (bd, 1H), 4.70 (bd, 1H), 3.86 (s, 3H).

Example 8 Preparation of 3-[(4-Chloro-2,5-dimethyl-benzenesulfonyl)-(3-iodo-benzyl)-amino]-5-phenyl-thiophene-2-carboxylic acid Compound #1 and 3-[(3-Benzofuran-2-yl-benzyl)-(4-chloro-2,5-dimethyl-benzenesulfonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid compound #2

Step I

To a solution of 3-(4-Chloro-2,5-dimethyl-benzenesulfonylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester (100 mg, 0.229 mmol) in anhydrous DMF (6 mL), 3-iodobenzyl bromide (82 mg, 0.276 mmol) and cesium carbonate (88 mg, 0.276 mmol) were added and the reaction mixture was stirred at room temperature under a N₂ atmosphere for 12 h. The reaction mixture was partitioned between water and ether. The ether layer was separated, dried (Na₂SO₄), concentrated. The residue was purified by silica gel column chromatography using ethyl acetate and hexane (1:3) as eluent to obtain 3-[(4-Chloro-2,5-dimethyl-benzenesulfonyl)-(3-iodo-benzyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (130 mg, 87%) as a syrup.

Step II

3-[(4-Chloro-2,5-dimethyl-benzenesulfonyl)-(3-iodo-benzyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (25 mg, 0.038 mmol) was taken in a mixture of THF:MeOH:H₂O (3:2:1, 3 mL) and then added 1N aqueous solution of LiOH.H₂0 (0.24 mL, 0.228 mmol). The reaction mixture was stirred at room temperature for 12 h. Solvents were removed and the residue was partitioned between water and ethyl acetate. The aqueous layer was acidified using 10% KHSO₄ solution. The organic layer was separated, dried (Na₂SO₄) and concentrated. The residue was purified by silica gel column chromatography using dichloromethane and methanol (9:1) to obtain 3-[(4-Chloro-2,5-dimethyl-benzene-sulfonyl)-(3-iodo-benzyl)-amino]-5-phenyl-thiophene-2-carboxylic acid (22 mg, 88%) as a white solid. ¹H NMR (CDCl₃, 400 MHz): 7.69 (m, 3H), 7.57 (m, 3H), 7.42 (m, 3H), 7.33 (d, 1H), 7.16 (s, 1H), 6.04 (dd, 1H), 4.90 (bs, 2H), 2.36 (s, 6H).

Compound #5 was prepared in a similar manner;

-   3-[(3-Benzofuran-2-yl-benzyl)-(4-chloro-2,5-dimethyl-benzenesulfonyl)-amino]-5-phenyl-thiophene-2-carboxylic     acid compound #2

Step I

To a degassed solution of 3-[(4-Chloro-2,5-dimethyl-benzenesulfonyl)-(3-iodo-benzyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (110 mg, 0.169 mmol) and benzofuran-2-boronic acid (55 mg, 0.185 mmol) in a mixture of DME (8 mL) and 2M aqueous Na₂CO₃ (4 mL), Pd(PPh₃)₄ (9 mg) was added and the reaction mixture was stirred at reflux conditions for 2 h under a N₂ atmosphere. The reaction mixture was diluted with ethyl acetate and water. The organic layer was separated, dried (Na₂SO₄) and concentrated. 3-[(3-Benzofuran-2-yl-benzyl)-(4-chloro-2,5-dimethyl-benzenesulfonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (107 mg, 100%) was isolated as a thick syrup and used for the next reaction without any further purification.

Step II

3-[(3-Benzofuran-2-yl-benzyl)-(4-chloro-2,5-dimethyl-benzenesulfonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (20 mg, 0.031 mmol) was taken in a mixture of THF:MeOH:H₂O (3:2:1, 3 mL) and then added 1N aqueous solution of LiOH.H₂0 (0.20 mL, 0.186 mmol). The reaction mixture was stirred at room temperature for 12 h. Solvents were removed and the residue was partitioned between water and ethyl acetate. The aqueous layer was acidified using 10% KHSO₄ solution. The organic layer was separated, dried (Na₂SO₄) and concentrated. The residue was purified by silica gel column chromatography using dichloromethane and methanol (9:1) to obtain 3-[(3-Benzofuran-2-yl-benzyl)-(4-chloro-2,5-dimethyl-benzene-sulfonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid (14 mg, 70%) as a white solid. ¹H NMR (DMSO, 400 MHz): 67.93 (s, 1H), 7.84 (s, 1H), 7.74 (bd, 1H), 7.65-7.22 (m, 14H), 4.95 (s, 2H), 2.33, 2.23 (2s, 6H).

Example 9 3-[(4-Chloro-benzoyl)-isopropyl-amino]-5-phenyl-thiophene-2-carboxylic acid compound #210

Step I

Method A

A DMF (15 mL) solution of 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (500 mg, 21.5 mmol) was cooled to 0° C. and then isopropyl iodide (2.57 mL) and NaH (60%, 775 mg, 32.3 mmol) were added under an atmosphere of N₂. The ice bath was removed and the reaction mixture was stirred at room temperature for 1 h. The mixture was partitioned between ether and water, the ether layer was separated, dried (Na₂SO₄) and concentrated. The residue was purified by silica gel column chromatography using ethyl acetate and hexane (5:95) as eluent to obtain 3-isopropylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester (189 mg, 32%) as a solid. ¹H NMR (CDCl₃, 400 MHz): 7.62 (d, 2H), 7.40 (m, 3H), 6.91 (s, 1H), 3.84 (s, 3H), 1.35 (d, 6H).

Method B

To a stirred solution of 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (1.82 g, 7.8 mmol) in 1,2-dichloroethane (40 mL) was added sequentially 2-methoxypropene (3.0 mL, 31.2 mmol), AcOH (1.8 mL, 31.2 mmol) and NaBH(OAc)₃ (3.31 g, 15.6 mmol) and stirred for 2 hrs. It was then diluted with EtOAc and H₂O. The aqueous solution was adjusted to pH=7 by adding NaHCO₃. The aqueous phase was extracted with EtOAc, the combined extract was washed with brine and dried on MgSO4 and filtered. Purification on bond elute with hexane to 5% EtOAc-hexane furnished 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (2.07 g, 96% yield).

The intermediate compounds 3-Cyclohexylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester, 3-(1-Methyl-piperidin-4-ylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester and 3-(1-Methyl-piperidin-4-ylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester were prepared in a similar manner as described and used as intermediates in the synthesis of compound #543, compound #553 and compound #573

Step II

To a suspension of 3-isopropylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester (1.2 g, 4.364 mmol) in a mixture of H₂O (22 mL) and dioxane (35 mL), 1N aqueous solution of NaOH (13 mL, 13.00 mmol) was added. The reaction mixture was stirred at 100° C. for 3 h. The reaction mixture was used for the next reaction without any further purification.

To this reaction mixture of 3-Amino-5-phenyl-thiophene-2-carboxylic acid sodium salt (23 mL, 1.41 mmol), 4-chlorobenzoyl chloride (0.269 mL, 2.11 mmol) was added at 0° C. The pH of the solution was maintained at 9 by adding 1N NaOH solution and then stirred at room temperature for 5 h. The reaction mixture was diluted with ethyl acetate and water. The water layer was acidified by adding 1N HCl solution. The organic layer was separated, dried (Na₂SO₄) and concentrated. The crude product was purified by recrystallization from ethyl acetate to obtain the pure 3-[(4-Chloro-benzoyl)-isopropyl-amino]-5-phenyl-thiophene-2-carboxylic acid (45 mg) as a white solid. ¹H NMR (DMSO-D₆, 400 MHz): 7.58 (d, 2H), 7.38-7.26 (m, 6H), 7.13 (d, 1H), 4.77 (m, 1H), 1.25 (d, 3H), 1.02 (d, 3H). ESI⁻ (M-H): 398.

Similarly, the following compounds were made: Compound #218, Compound #219, Compound #226, Compound #234, Compound #243, Compound #246, Compound #250, Compound #262, Compound #324, Compound 326, Compound #331.

Example 10 3-[(2,4-Dichloro-benzoyl)-isopropyl-amino]-5-phenyl-thiophene-2-carboxylic acid compound #149

Step I

3-(2,4-Dichloro-benzoylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester

To a ice-cold solution of 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester 1 (5 g, 21.5 mmol) and triethylamine (4.56 g, 45.0 mmol) in dichloromethane (100 ml) was added 2,4-dichlorobenzoyl chloride (3.90 g, 19.4 mmol). The reaction mixture was stirred for 30 min a 0° C. and 16 h at room temperature. Then, the reaction mixture was partitioned between 25 ml of H₂O, 50 ml sat. NaHCO₃ and 50 ml of CH₂Cl₂. The organic layer was separated and the aqueous phase was washed twice with CH₂Cl₂ (2×50 mL). The combined dichloromethane layer was dried (MgSO₄), concentrated and the residue was purified by recrystallization in CH₂Cl₂ to obtain 5.832 g (74%) as a white solid of 3-(2,4-Dichloro-benzoylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester. NMR ¹H (CDCl₃, 400 MHz): 8.30 ppm (s, 1H, H_(aro)); 7.74-7.66 ppm (m, 3H, H_(aro)); 7.51 ppm (d, 1H, H_(aro)); 7.46-7.34 ppm (m, 4H, H_(aro)); 3.91 ppm (s, 3H).

Step II

3-[(2,4-Dichloro-benzoyl)-isopropyl-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester

Sodium Hydride (60% dispersion in oil, 190 mg, 5.2 mmol) was added to an ice-cold solution of 3-(2,4-Dichloro-benzoylamino)-5-phenyl-thiophene-2-carboxylic acid methyl ester (2) (1.5 g, 3.69 mmol) in 350 ml of N,N-dimethylformamide in an atmosphere of N₂. After 5 min, 2-Iodo-propane (941 mg, 5.54 mmol) was added to the solution and then the reaction mixture was stirred for 30 min at 0° C. and 64 h at room temperature. The mixture was partitioned between ether (200 mL) and water (350 mL) and the organic layer was separated. The aqueous phase was washed twice with ether (2×70 mL) and the combined ether layer was dried (MgSO₄), concentrated and the residue was purified by flash chromatography (10% EtOAc/Hexane) to obtain 908 mg (55%) of 3-[(2,4-Dichloro-benzoyl)-isopropyl-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester. NMR ¹H (CDCl₃, 400 MHz): Rotamere 95/05:7.54 ppm (dd, 2H, H_(aro)); 7.49-7.35 ppm (m, 3H, H_(aro)); 7.29-7.25 ppm (m, 2H, H_(aro)); 7.15 ppm (d, 1H, H_(aro)); 7.05 ppm (d, 1H, H_(aro)) 5.09 ppm (hex, 1H, N—CH(CH₃), major rotamere); 3.99 ppm (hex, N—CH(CH₃), minor rotamere); 3.89 ppm (s, 3H); 1.40 ppm (d, 3H, N—CH(CH₃), major rotamere); 1.28 ppm (d, N—CH(CH₃), minor rotamere); 1.09 ppm (d, 3H, N—CH(CH₃), major rotamere); 1.01 ppm (d, N—CH(CH₃), minor rotamere).

Step III

3-[(2,4-Dichloro-benzoyl)-isopropyl-amino]-5-phenyl-thiophene-2-carboxylic acid

3-[(2,4-Dichloro-benzoyl)-isopropyl-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (3) (345 mg, 0.77 mmol) was dissolved in a mixture of THF-MeOH—H₂O (3:2:1) (30 mL) and then 4.6 ml of LiOH 1N was added to it. After 120 min of stiffing at room temperature, solvant was removed and then partitioned between 25 ml of H₂O, 4 ml of KHSO₄ 5% and 25 ml of EtOAc. The organic layer was separated and the aqueous phase was washed twice with ethyl acetate (2×10 mL). The combined ethyl acetate layer was dried (MgSO₄), concentrated and the residue was purified by preparative chromatography (10% MeOH/CH₂Cl₂) to obtain 175 mg (53%) as a white solid of 3-[(2,4-Dichloro-benzoyl)-isopropyl-amino]-5-phenyl-thiophene-2-carboxylic acid. NMR ¹H (DMSO, 400 MHz): Rotamer 95/05:7.82 ppm (m, H_(aro), minor rotamer); 7.69 ppm (d, 2H, H_(aro)); 7.61 ppm (d, 1H, H_(aro)); 7.51-7.37 ppm (m, 4H, H_(aro)); 7.35-7.28 ppm (m, 2H, H_(aro)); 4.89 ppm (hex, 1H, N—CH(CH₃), major rotamer); 3.84 ppm (hex, N—CH(CH₃), minor rotamer); 1.36 ppm (d, 3H, N—CH(CH₃), major rotamer); 1.25 ppm (d, N—CH(CH₃), minor rotamer); 1.03 ppm (d, 3H, N—CH(CH₃), major rotamer); 0.93 ppm (d, N—CH(CH₃), minor rotamere).

The following compounds were prepared in a similar manner: Compound #201, Compound #204, Compound #233, Compound #244, Compound #261, Compound #264, Compound #299.

Example 11 3-[(2,4-Dichloro-benzoyl)-phenyl-amino]-5-phenyl-thiophene-2-carboxylic acid. Compound #208

Step I

5-Phenyl-3-phenylamino-thiophene-2-carboxylic acid methyl ester

To a solution of 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (1 g, 4.29 mmol) in dichloromethane (50 ml) was added phenyl boronic acid (1.05 g, 8.6 mmol), pyridine (680 mg, 8.6 mmol) and copper(II) acetate (1.18 g, 6.5 mmol). The reaction mixture was stirred for 16 h at room temperature. Then, the reaction mixture was filtered through celite, concentrated and the residue was purified by flash chromatography (9:1 Hexane/EtOAc) to obtain 435 mg (33%) of 5-Phenyl-3-phenylamino-thiophene-2-carboxylic acid methyl ester. NMR ¹H (CDCl₃, 400 MHz): 7.38 ppm (dd, 2H, H_(aro)); 7.35-7.26 ppm (m, 5H, H_(aro)); 7.19 ppm (s, 1H, H_(aro)); 7.15 ppm (dd, 2H, H_(aro)); 7.02 ppm (ddt, 1H, H_(aro)); 3.82 ppm (s, 3H).

Step II

3-[(2,4-Dichloro-benzoyl)-phenyl-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester

Sodium Hydride (60% dispersion in oil, 80 mg, 1.5 mmol) was added to an ice-cold solution 5-Phenyl-3-phenylamino-thiophene-2-carboxylic acid methyl ester (2) (230 mg, 0.74 mmol) in 20 ml of N,N-dimethylformamide in an atmosphere of N₂. After 5 min, 2,4-Dichloro-benzoyl chloride (310 mg, 1.48 mmol) was added to the solution and then the reaction mixture was stirred for 30 min at 0° C. and 16 h at room temperature. The mixture was partitioned between ether (20 mL) and water (20 mL) and the organic layer was separated. The aqueous phase was washed twice with ether (2×10 mL) and the combined ether layer was dried (MgSO₄), concentrated and the residue was purified by preparative chromatography (30% EtOAc/Hexane) to obtain 58 mg (16%) of 3-[(2,4-Dichloro-benzoyl)-phenyl-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester. NMR ¹H (CDCl₃, 400 MHz): 7.65-7.10 ppm (m, 14H, H_(aro)); 3.77 ppm (s, 3H).

Step III

3-[(2,4-Dichloro-benzoyl)-phenyl-amino]-5-phenyl-thiophene-2-carboxylic acid.

3-[(2,4-Dichloro-benzoyl)-phenyl-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (55 mg, 0.11 mmol) was dissolved in a mixture of THF-MeOH—H₂O (3:2:1) (15 mL) and then 0.66 ml of LiOH 1N was added to it. After 60 min of stirring at room temperature, solvents were removed and then partitioned between 15 ml of H₂O, 4 ml of KHSO₄ 5% and 15 ml of EtOAc. The organic layer was separated and the aqueous phase was washed twice with ethyl acetate (2×10 mL). The combined ethyl acetate layer was dried (MgSO₄), concentrated and the residue was purified by preparative chromatography (10% MeOH/CH₂Cl₂) to obtain 32 mg (60%) of 3-[(2,4-Dichloro-benzoyl)-phenyl-amino]-5-phenyl-thiophene-2-carboxylic acid. NMR ¹H (DMSO, 400 MHz): Rotamer: 7.75 ppm (d, 1H, H_(aro)); 7.68 ppm (2H, H_(aro)); 7.53 ppm (d, H_(aro), minor rotamer); 7.51-7.23 ppm (m, 11H, H_(aro), minor rotamer); 7.17 ppm (H_(aro), minor rotamer).

Compound #525 was prepared in a similar manner.

Example 12 3-[tert-Butyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid compound #327

Step I

3-tert-Butylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester

Concentrated sulfuric acid (10 drop) was added to a solution of 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (500 mg, 2.15 mmol) in 20 ml of dioxane/chloroforme (2:3) in a sealed tube. After cooling the solution at −78° C., put 20 ml of isobutene gaz. The sealed tube was closed and then the reaction mixture was stirred for 6 days at 60° C. The solvant was removed and then partitioned between 15 ml of sat. Na₂CO₃ solution and 15 ml of EtOAc. The organic layer was separated, the aqueous phase was washed twice with ethyl acetate and the combined ethyl acetate layer was dried (MgSO₄), concentrated and the residue was purified by flash chromatography (5% EtOAc/Hexane) to obtain 385 mg (62%) of 3-tert-Butylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester. NMR ¹H (CDCl₃, 400 MHz): 7.65 ppm (d, 2H, H_(aro)); 7.44-7.38 ppm (m, 3H, H_(aro)); 7.07 ppm (s, 1H, H_(aro)); 3.86 ppm (s, 3H); 1.48 ppm (s, 9H).

Step II

3-[tert-Butyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester

To a solution of 3-tert-Butylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester (100 mg, 0.35 mmol) in dichloroethane (10 ml) in an atmosphere of N₂ was added 2,4-dichloro-benzoyl chloride (79 mg, 0.38 mmol). The reaction mixture was stirred for 16 h at reflux. Then, the solvents were removed and the residue was purified by flash chromatography (9:1 Hexane/EtOAc) to obtain 112 mg (69%) of 3-[tert-Butyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester. NMR ¹H (CDCl₃, 400 MHz): 7.50 ppm (m, 2H, H_(aro)); 7.44-7.34 ppm (m, 3H, H_(aro)); 7.27 ppm (s, 1H, H_(aro)); 7.18 ppm (dl, 1H, H_(aro)); 7.14 ppm (d, 1H, H_(aro)); 7.00 ppm (dd, 1H, H_(aro)); 3.93 ppm (s, 3H); 1.56 ppm (s, 9H).

Step III

3-[tert-Butyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid

3-[tert-Butyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (112 mg, 0.24 mmol) was dissolved in a mixture of THF-MeOH—H₂O (3:2:1) (15 mL) and then 1.5 ml of LiOH 1N was added to it. After 3 h of stirring at room temperature, solvant was removed and then partitioned between 15 ml of H₂O, 4 ml of KHSO₄ 5% and 15 ml of EtOAc. The organic layer was separated and the aqueous phase was washed twice with ethyl acetate (2×10 mL). The combined ethyl acetate layer was dried (MgSO₄), concentrated and the residue was purified by preparative chromatography (10% MeOH/CH₂Cl₂) to obtain 32 mg (29%) of 3-[tert-Butyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid. NMR ¹H (DMSO, 400 MHz): 7.62 ppm (d, 2H, H_(aro)); 7.44-7.34 ppm (m, 4H, H_(aro)); 7.32-7.12 ppm (m, 3H, H_(aro)); 2.48 ppm (s, 9H).

Example 13 3-[Cyclopropyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid. Compound #333

Step I

3-Cyclopropylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester

To a solution of 3-Bromo-5-phenyl-thiophene-2-carboxylic acid methyl ester (250 mg, 0.89 mmol) in toluene (25 ml) was added cyclopropylamine (57 mg, 1.0 mmol), cesium carbonate (382 mg, 1.2 mmol), BINAP (50 mg, 0.08 mmol) and tris(dibenzyli-denacetone)dipaladium (0) (38 mg, 0.04 mmol). The reaction mixture was stirred for 16 h at 110° C. in a sealed tube. The mixture was partitioned between toluene (20 mL) and water (20 mL) and the organic layer was separated. The aqueous phase was washed twice with toluene (2×10 mL) and the combined toluene layer was dried (MgSO₄), concentrated and the residue was purified by preparative chromatography (10% EtOAc/Hexane) to obtain 52 mg (22%) of 3-Cyclopropylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester. NMR ¹H (CDCl₃, 400 MHz): 7.67-7.62 ppm (m, 2H, H_(aro)); 7.43-7.32 ppm (m, 3H, H_(aro)); 7.16 ppm (s, 1H, H_(aro)); 3.82 ppm (s, 3H); 2.65 ppm (m, 1H); 0.62 ppm (m, 2H); 0.35 ppm (m, 2H).

Step II 3-[Cyclopropyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester

To a solution of 3-Cyclopropylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester (52 mg, 0.19 mmol) in dichloroethane (10 ml) in an atmosphere of N₂ was added 2,4-dichlorobenzoyl chloride (45 mg, 0.21 mmol). The reaction mixture was stirred for 16 h at reflux. Then, the solvant was removed and the residue was purified by flash chromatography (8:2 Hexane/EtOAc) to obtain 85 mg (99%) of 3-[Cyclopropyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester. NMR ¹H (CDCl₃, 400 MHz): 7.64 ppm (d, 2H, H_(aro)); 7.47 ppm (m, 2H, H_(aro)); 7.44-7.33 ppm (m, 3H, H_(aro)); 7.21-7.12 ppm (m, 2H, H_(aro)); 3.89 ppm (s, 3H); 3.33 ppm (m, minor rotamer); 3.13 ppm (m, 1H, major rotamer) 1.01-0.49 ppm (m, 4H).

Step III

3-[Cyclopropyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid

3-[Cyclopropyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (85 mg, 0.19 mmol) was dissolved in a mixture of THF-MeOH—H₂O (3:2:1) (10 mL) and then 1.2 ml of LiOH 1N was added to it. After 60 min of stirring at room temperature, solvant was removed and then partitioned between 15 ml of H₂O, 4 ml of KHSO₄ 5% and 15 ml of EtOAc. The organic layer was separated and the aqueous phase was washed twice with ethyl acetate (2×10 mL). The combined ethyl acetate layer was dried (MgSO₄), concentrated and the residue was purified by preparative chromatography (10% MeOH/CH₂Cl₂) to obtain 22 mg (27%) of 3-[Cyclopropyl-(2,4-dichloro-benzoyl)-amino]-5-phenyl-thiophene-2-carboxylic acid. NMR ¹H (DMSO, 400 MHz): rotamer: 7.75 ppm (m, 2H, H_(aro)); 7.68 ppm (m, H_(aro)), minor rotamer); 7.62-7.55 ppm (m, 2H, H_(aro)); 7.52 ppm (m, H_(aro), minor rotamer); 7.48-7.27 ppm (m, 5H, H_(aro)); 3.14 ppm (m, minor rotamer); 3.04 ppm (m, 1H, major rotamer); 0.87-0.42 ppm (m, 4H).

The following compounds were prepared in a similar manner:

Compound #403, Compound #404 Example 14 3-[(2,4-dichloro-benzoyl)-piperidin-4-ylmethylamino]-5-phenyl-thiophene-2-carboxylic acid Compound #519

Step I

A suspension of 3-amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (0.70 g, 3 mmol) and 4-formyl N-Cbz-piperidine (0.74 g, 3 mmol) in THF (1.2 mL) was treated with dibutyltin dichloride (46 mg, 0.15 mol) followed by phenylsilane (0.41 mL, 3.3 mmol). The mixture was stirred for 2 days at room temperature. The solvent was then evaporated and the residue was purified by silica gel column chromatography using CH₂Cl₂:hexanes:EtOAc as eluent to provide 4-[(2-Methoxycarbonyl-5-phenyl-thiophen-3-ylamino)-methyl]-piperidine-1-carboxylic acid benzyl ester (0.6906 g, 50% yield).

Step II

4-[(2-Methoxycarbonyl-5-phenyl-thiophen-3-ylamino)-methyl]-piperidine-1-carboxylic acid benzyl ester (133 mg, 0.28 mmol) was dissolved in 1,2-dichloroethane (2.8 mL) and was treated with 2,4-dichlorobenzoyl chloride (60 L, 0.43 mmol). The solution was heated at reflux for 1 day. The solvent was then evaporated and the residue purified by silica gel column chromatography using hexanes:EtOAc as eluent to provide 4-{[(2,4-Dichloro-benzoyl)-(2-methoxycarbonyl-5-phenyl-thiophen-3-yl)-amino]-methyl}-piperidine-1-carboxylic acid benzyl ester (0.156 g, 85% yield).

Step III

4-{[(2,4-Dichloro-benzoyl)-(2-methoxycarbonyl-5-phenyl-thiophen-3-yl)-amino]-methyl}-piperidine-1-carboxylic acid benzyl ester (150 mg, 0.24 mmol) was dissolved in a mixture of THF:MeOH:H₂O (3:2:1, 2.4 mL) and treated with LiOH.H₂O (29.6 mg, 0.7 mmol). The solution was heated at 55° C. for 2 h. The solvents were removed and the residue was acidified using HCl. The product was extracted with EtOAc and the organic layers were washed with brine and dried. The residue was purified by silica gel column chromatography using EtOAc:MeOH:AcOH as eluent to provide 4-{[(2-Carboxy-5-phenyl-thiophen-3-yl)-(2,4-dichloro-benzoyl)-amino]-methyl}-piperidine-1-carboxylic acid benzyl ester (124 mg, 85% yield).

Step IV

4-{[(2-Carboxy-5-phenyl-thiophen-3-yl)-(2,4-dichloro-benzoyl)-amino}-methyl]-piperidine-1-carboxylic acid benzyl ester (124 mg, 0.2 mmol) was dissolved in MeOH (2 mL) and treated with 10% Pd/C (200 mg) under H₂ balloon. The reaction was stirred at room temperature for 18 h and the mixture was filtered on celite. The solution was evaporated to a residue that was purified by reverse-phase HPLC to provide 3-[(2,4-Dichloro-benzoyl)-piperidin-4-ylmethyl-amino]-5-phenyl-thiophene-2-carboxylic acid (17.3 mg, 18% yield). ¹H NMR (CD₃OD, 300 MHz): 7.55 (d, 1H), 7.50 (m, 2H), 7.27-7.39 (m, 4H), 7.25 (s, 1H), 7.18 (dd, 1H), 4.12 (m, 1H), 3.75 (m, 1H), 3.43 (m, 2H), 2.96 (q, 2H), 2.65 (d, 2H), 2.05 (m, 1H), 1.62 (m, 2H).

The following compounds were prepared in a similar manner: Compound #503, Compound #509, Compound #519, Compound #529, Compound #537, Compound #538, Compound #516, Compound #522, Compound #535.

Example 15 3-[Isopropyl-(3-methyl-cyclopent-3-enecarbonyl)-amino]-5 phenyl-thiophene-2-carboxylic acid

Compound #405

Step I

To a cold (−78° C.) stirred solution of LDA (generated from DIPA (1.42 mL, 10.14 mmol), BuLi (5.85 mL, 9.36 mmol) in THF at −78° C. for 20 min) in THF (31 mL) was added a solution of Pent-4-enoic acid ethyl ester (1.0 g, 7.8 mmol, 1.2 eq.) in THF (9.0 mL). After stirred for 1 h, neat 3-Bromo-2-methyl-propene (2.03 g, 15.0 mmol, 1.51 mL) was added and slowly warmed up to room temperature for overnight. The reaction mixture was then quenched with saturated NH₄Cl solution, extracted with ether, washed with brine and dried. Evaporation of the solution furnished the 2-Allyl-4-methyl-pent-4-enoic acid ethyl ester (1.45 g, 100%) as an oil which was used in the next step without purification. ¹H NMR (400 MHz, CDCl₃), 5.78-5.71 (m, 1H), 5.05 (d, J=18.6 Hz, 1H), 5.02 (d, J=9.4 Hz, 1H), 4.76 (brs, 1H), 4.70 (s, 1H), 4.11 (dq, J=7.2, 1.0 Hz, 2H), 2.66-2.13 (m, 5H), 1.72 (s, 3H), 1.23 (dt, J=7.2, 1.3 Hz, 3H).

Step II

To a refluxing stirred solution of the 2-Allyl-4-methyl-pent-4-enoic acid ethyl ester (364 mg, 2.0 mmol) in CH₂Cl₂ (100 mL, 0.02 M solution) was added drop wise a solution of the tricyclohexylphosphine (1,3-Bis(2,4,6-trimethylphenyl)-4,5-dihydroimidazol-2-ylidene)(benzylidine)ruthenium (IV)dichloride (85 mg, 0.1 mmol) in CH₂Cl₂ (3.0 mL). After 50 min, the reaction mixture was cooled to room temperature, concentrated and purified on silica gel bond elute using EtOAc/hexane (1:20) as an eluent furnished the 3-Methyl-cyclopent-3-enecarboxylic acid ethyl ester (286 mg, 93% yield) as an oil. ¹H NMR (CDCl₃, 400 MHz), 5.25 (brs, 1H), 4.17 (q, J=7.1 Hz, 2H), 3.2-3.1 (m, 1H), 2.65-2.46 (m, 4H), 1.74 (s, 3H), 1.28 (t, J=7.1 Hz, 3H).

Step III

A solution of the 3-Methyl-cyclopent-3-enecarboxylic acid ethyl ester (255 mg, 1.65 mmol) in MeOH (4.0 mL) and 10% aq. NaOH (3.3 mL, 8.25 mmol) was heated at 50° C. for 16 h, reaction mixture was cooled to room temperature, solvent was evaporated, diluted with water. The aqueous solution was washed with ether, and acidified with aq. 1 N HCl, extracted with ether. The ethereal solution was washed with brine and dried. Evaporation of the solvent furnished the 3-Methyl-cyclopent-3-enecarboxylic acid (200 mg, 97% yield). 1H NMR (CDCl₃, 400 MHz) 5.27 (brs, 1H), 3.26-3.17 (m, 1H), 2.7-2.55 (m, 4H), 1.74 (s, 3H).

Step IV

The coupling of the 3-Isopropylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester (82 mg, 0.3 mmol) and the 3-Methyl-cyclopent-3-enecarboxylic acid (45 mg, 0.357 mmol) using PPh₃ (95.4 mg, 0.363 mmol) and NCS (48.5 mg, 0.363 mmol) furnished the 3-[Isopropyl-(3-methyl-cyclopent-3-enecarbonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (70 mg, 61% yield) ¹H NMR (CDCl₃, 400 MHz 1:1 mixture of rotamers), 7.68-7.64 (m, 4H), 7.5-7.4 (m, 6H), 7.1 (s, 1H), 7.09 (s, 1H), 5.2 (s, 1H), 5.1 (s, 1H), 5.06-4.98 (m, 2H), 3.88 (s, 3H), 3.87 (s, 3H), 3.08-3.0 (m, 2H), 2.85-2.76 (m, 2H), 2.5-2.42 (m, 2H), 2.3-2.1 (m, 4H), 1.69 (s, 3H), 1.64 (s, 3H), 1.24 (d, J=6.7 Hz, 3H), 1.23 (d, J=6.7 Hz, 3H), 1.01 (d, J=6.9 Hz, 3H), 1.007 (d, J=6.8 Hz, 3H).

Saponification of 3-[Isopropyl-(3-methyl-cyclopent-3-enecarbonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (50 mg, 0.13 mmol) using LiOH.H₂O (22 mg) as previously described furnished the 3-[Isopropyl-(3-methyl-cyclopent-3-enecarbonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid (30 mg, 62.5% yield) as a solid.

¹H NMR (CD₃OD, 400 MHz 1:1 mixture of rotamers) 7.73-7.70 (m, 4H), 7.47-7.35 (m, 6H), 7.29 (s, 1H), 7.27 (s, 1H), 5.16 (s, 1H), 5.08 (s, 1H), 4.9-4.8 (m, 2H), 3.15-3.05 (m, 2H), 2.76-2.65 (m, 2H), 2.42-2.12 (m, 6H), 1.65 (s, 3H), 1.61 (s, 3H), 1.25 (d, J=6.6 Hz, 3H), 1.24 (d, J=6.6 Hz, 3H), 1.03 (d, J=6.9 Hz, 6H).

Example 16 5-tert-Butyl-3-(2,4-dimethyl-benzenesulfonylamino)-thiophene-2-carboxylic acid Compound #315

Step I

A mixture of 3-Amino-5-tert-butyl-thiophene-2-carboxylic acid methyl ester (106.5 mg, 0.5 mmol) and 2,4-dimethylsulfonyl chloride (156 mg, 0.75 mmol) in pyridine (1.5 mL) was heated at 72° C. for 16 h. The reaction mixture was diluted with EtOAc, washed with aq. 1N HCl, brine and dried. Evaporation of the solvent and purification of the residue on silica gel bond elute using EtOAc (1:20 to 1:10) as an eluent furnished the 5-tert-Butyl-3-(2,4-dimethyl-benzenesulfonylamino)-thiophene-2-carboxylic acid methyl ester (188 mg, 99% yield). ¹H NMR (CDCl₃, 400 MHz) 9.73 (s, 1H), 7.89 (d, J=8.6 Hz, 1H), 7.04-7.08 (m, 2H), 7.03 (s, 1H), 3.82 (s, 3H), 2.62 (s, 3H), 2.33 (s, 3H), 1.28 (s, 9H).

Step II

Hydrolysis of the 5-tert-Butyl-3-(2,4-dimethyl-benzenesulfonylamino)-thiophene-2-carboxylic acid methyl ester (55 mg, 0.14 mmol) using LiOH.H₂O (22 mg) as previously described provided the 5-tert-Butyl-3-(2,4-dimethyl-benzenesulfonylamino)-thiophene-2-carboxylic acid (36 mg, 70% yield) as a solid. ¹H NMR (CD₃OD, 400 MHz) 7.85 (d, J=8.6 Hz, 1H), 7.14-7.10 (m, 2H), 7.0 (s, 1H), 2.56 (s, 3H), 2.31 (s, 3H), 1.27 (s, 9H).

Example 17 5-Benzo[b]thiophen-2-yl-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid Compound #230

Step I

Suzuki coupling of 5-Bromo-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester (43 mg, 0.1 mmol) and benzothiophene-2-boronic acid (53.4 mg, 0.3 mmol) was carried out using Pd(PPh₃)₄ and Na₂CO₃ (as described in example 2) resulted in 5-Benzo[b]thiophen-2-yl-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester (27 mg, 55% yield). ¹H NMR (CDCl₃, 400 MHz) 9.92 (s, 1H), 8.07 (d, J=7.8 Hz, 1H), 7.79-7.71 (m, 2H), 7.45-7.24 (m, 7H), 2.7 (s, 3H), 1.56 (s, 9H).

Step II

5-Benzo[b]thiophen-2-yl-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester was hydrolyzed to the acid using TFA as described for example 2 providing 5-Benzo[b]thiophen-2-yl-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid (24 mg, 99% yield). ¹H NMR (DMSO-D₆, 400 MHz) 10.19 (s, 1H), 8.0 (d, J=7.7 Hz, 1H), 7.79-7.74 (m, 1H), 7.86 (s, 1H), 7.84-7.81 (m, 1H), 7.54 (t, J=7.7 Hz, 1H), 7.53-7.36 (m, 4H), 7.32 (s, 1H), 2.58 (s, 3H).

Example 18 5-(1H-Pyrazol-3-yl)-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid Compound #170

Step I

To a stirred solution of 5-Bromo-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester (43 mg, 0.1 mmol) in toluene (3.0 mL) was sequentially added a solution of Pd(PPh3)₄ (12 mg, 0.01 mmol) in toluene (1.0 mL) and 3-Trimethylstannanyl-pyrazole-1-sulfonic acid dimethylamide (prepared according to J. Med. Chem. (1998), 41, p-2019) (75 mg, 0.2 mmol, 2.0 eq), and heated the resulting overnight at 80° C. It was then cooled to room temperature, the solvent was evaporated and the crude was purified on preparative TLC using EtOAc/hexane (1:5). 5-(1-Dimethylsulfamoyl-1H-pyrazol-3-yl)-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester (35 mg, 66.5% yield) was isolated.

¹H NMR (CDCl₃, 400 MHz) 9.93 (s, 1H), 8.11 (d, J=0.7 Hz, 1H), 8.02 (dd, J=6.7, 1.32 Hz, 1H), 7.84 (d, J=0.7 Hz, 1H), 7.45 (dt, J=7.5, 1.3 Hz, 1H), 7.31 (t, J=8.2 Hz, 2H), 7.26 (d, J=1.0 Hz, 1H), 2.98 (s, 6H), 2.7 (s, 3H), 1.55 (s, 9H).

Step II

A reaction mixture of 5-(1-Dimethylsulfamoyl-1H-pyrazol-3-yl)-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid tert-butyl ester (10 mg, 0.019 mmol) and 4N HCl (0.3 mL) solution in dioxane in MeOH (0.3 mL) was stirred at room temperature 26 h. Reaction mixture was then diluted with water and extracted with EtOAc, concentrated and purified on preparative TLC using MeOH/CH₂Cl₂/AcOH (5:95:1) furnished the 5-(1H-Pyrazol-3-yl)-3-(toluene-2-sulfonylamino)-thiophene-2-carboxylic acid (4.5 mg, 65.2% yield). ¹H NMR (CD₃OD, 400 MHz) 7.99 (d, J=7.9 Hz, 1H), 7.81 (s, 1H), 7.43 (t, J=7.5, 1.3 Hz, 1H), 7.42-7.26 (m, 2H), 7.19 (s, 1H), 2.69 (s, 3H).

Example 19 3-Isopropyl-[(4-methyl-cyclohexanecarbonyl)-amino]-5-m-tolyl-thiophene-2-carboxylic acid Compound #448

Step I

Trans-4-methyl-cyclohexanecarbonyl chloride was prepared by heating to reflux trans-4-methyl-cyclohexanecarboxylic acid (5 g, 0.035 mmol) in thionylchloride (5.0 ml) for 2 h followed by purification of the corresponding acyl chloride under reduced pressure in a Kugel-Rhorr apparatus collecting the fraction distilling at 95° C. yielding 5.1 g of the desired material which was used in the next step without further purification. This acyl chloride (1.5 ml, aprox. 10 mmol) was dissolved along with 5-Bromo-3-isopropylamino-thiophene-2-carboxylic acid methyl ester (2 g, 7.12 mmol) in anhydrous dichloroethane (2 mL) and heated at 80° C. (closed vial) for 12 h. The solvents were evaporated, the resulting crude material was dissolved in methanol and left 30 min. at room temperature, concentrated and purified via flash chromatography on silica gel using a 5% EtOAc 95% hexanes mixture of eluents, in this manner 600 mg (21%) of 5-Bromo-3-[isopropyl-(4-methyl-cyclohexanecarbonyl)-amino]-thiophene-2-carboxylic acid methyl ester the was isolated. ¹H NMR (CDCl₃, 300 MHz): 6.78 (s, 1H), 4.93 (m, 1H), 3.69 (s, 3H), 2.00-1.20 (m, 8H), 1.14 (d, 3H), 0.93 (d, 3H), 0.81 (d, 3H), 0.72-0.70 (m, 2H).

Step II

To a degassed solution of 5-Bromo-3-[isopropyl-(4-methyl-cyclohexanecarbonyl)-amino]-thiophene-2-carboxylic acid methyl ester (100 mg, 0.249 mmol) and 3-methyl boronic acid (38 mg, 0.279 mmol) in a mixture of DME (6 mL) and 2M aqueous Na₂CO₃ (3 mL), Pd(PPh₃)₄ (12 mg) was added and the reaction mixture was stirred at reflux conditions for 12 h under a N₂ atmosphere. The reaction mixture was diluted with ethyl acetate and water. The organic layer was separated, dried (Na₂SO₄), concentrated. The residue was purified by column chromatography using ethyl acetate and hexane (1:3) as eluent. 35 mg (34%) of 3-Isopropyl-[(4-methyl-cyclohexanecarbonyl)-amino]-5-m-tolyl-thiophene-2-carboxylic acid methyl ester was isolated. ¹H NMR (CDCl₃, 400 MHz): 7.45 (bs, 2H), 7.36 (t, 1H), 7.23 (m, 1H), 7.01 (s, 1H), 4.99 (m, 1H), 3.83 (s, 3H), 2.41 (s, 3H), 2.01-0.61 (m, 20H).

Step III

3-Isopropyl-[(4-methyl-cyclohexanecarbonyl)-amino]-5-m-tolyl-thiophene-2-carboxylic acid methyl ester (30 mg, 0.073 mmol) was taken in a mixture of THF:MeOH:H₂O (3:2:1, 3 mL) and then added 1N aqueous solution of LiOH.H₂O (0.44 mL, 0.438 mmol). The reaction mixture was stirred at room temperature for 12 h. Solvents were removed and the residue was partitioned between water and ethyl acetate. The aqueous layer was acidified using 10% KHSO₄ solution. The organic layer was separated, dried (Na₂SO₄) and concentrated. The residue was purified by preparative TLC using chloroform:methanol:acetic acid (9:1:0.1) to obtain 3-Isopropyl-[(4-methyl-cyclohexanecarbonyl)-amino]-5-m-tolyl-thiophene-2-carboxylic acid (15 mg, 52%) as a white solid. ¹H NMR (CDCl₃, 400 MHz): (s, 2H), 7.38 (t, 1H), 7.24 (m, 1H), 7.08 (s, 1H), 5.01 (s, 1H), 2.42 (s, 3H), 2.10-0.62 (m, 20H). ESI⁻ (M-H): 398.

Example 20 (1R,2S,4R)-3-[Isopropyl-(2-hydroxy-4-methyl-cyclohexanecarbonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid Compound #402

(1R,2S,4R)-2-Hydroxy-4-methyl-cyclohexanecarboxylic acid methyl ester was prepared as described in J. Org. Chem., (1993), 58, pp. 6255-6265. NMR ¹H (CDCl₃, 400 MHz): 4.26 ppm (s, 1H); 4.19-4.13 ppm (m, 2H); 3.16 ppm (s, 1H); 2.35-2.29 ppm (m, 1H); 1.92-1.74 ppm (m, 5H); 1.31-1.24 ppm (m, 3H); 1.08-1.01 ppm (m, 1H); 0.96-0.92 ppm (m, 1H); 0.88 ppm (d, 3H).

Step I

To a solution of (1R,2S,4R)-2-Hydroxy-4-methyl-cyclohexanecarboxylic acid methyl ester (450 mg, 2.42 mmol) in methanol (12 ml) was added a 2.5 M solution of sodium hydroxide (9.7 ml, 24.2 mmol). The reaction mixture was stirred for 4 h at 50° C. Then, the solvents were removed and the residue was partitioned between 20 ml of H₂O acidified to pH 4 and 20 ml of EtOAc. The organic layer was separated and the aqueous phase was washed with ethyl acetate (2×20 ml). The combined ethyl acetate layers were dried (Na₂SO₄) and concentrated to obtain 313 mg (82%) of (1R,2S,4R)-2-Hydroxy-4-methyl-cyclohexanecarboxylic acid. NMR ¹H (CDCl₃, 400 MHz): 4.34 ppm (s, 1H); 2.43-2.39 ppm (m, 1H); 1.96-1.76 ppm (m, 5H); 1.14-1.08 ppm (m, 1H); 1.02-0.93 ppm (m, 1H); 0.90 ppm (d, 3H).

Step II

To a solution of (1R,2S,4R)-2-Hydroxy-4-methyl-cyclohexanecarboxylic acid (162 mg, 1.02 mmol) in dichloromethane (5 ml) was added pyridine (495 ul, 6.12 mmol) followed by acetic anhydride (385 ul, 4.08 mmol). The reaction mixture was stirred for 20 h at room temperature. Then, the solvents were removed and 10 ml of 3N HCl solution was added. This mixture was stirred for 30 minutes and then a saturated solution of NaHCO₃ was slowly added until pH=9-10. This solution was then extracted with ethyl acetate (2×5 ml). The aqueous phase was then acidified with a 10% HCl solution and extracted with ethyl acetate (3×5 ml). The following ethyl acetate layers were combined, dried (Na₂SO₄) and concentrated to obtain 109 mg (53%) of (1R,2S,4R)-2-Acetoxy-4-methyl-cyclohexanecarboxylic acid.

NMR ¹H (CDCl₃, 400 MHz): 5.45 ppm (s, 1H); 2.46-2.42 ppm (m, 1H); 2.02 ppm (s, 3H); 2.02-1.96 ppm (m, 1H); 1.91-1.76 ppm (m, 3H); 1.70-1.61 ppm (m, 1H); 1.16-1.08 ppm (m, 1H); 0.99-0.88 ppm (m, 1H); 0.87 ppm (d, 3H).

Step III

To a solution of (1R,2S,4R)-2-Acetoxy-4-methyl-cyclohexanecarboxylic acid (109 mg, 0.54 mmol) in dichloromethane (2.7 ml) was added oxalyl chloride (545 l, 1.09 mmol) followed by 1 drop of dimethylformamide. The reaction mixture was stirred for 4 h at room temperature. The solvents were then removed to obtain 119 mg (99%) of (1R,2S,4R)-2-Acetoxy-4-methyl-cyclohexanecarboxylic acid chloride.

Step IV

To a solution of 3-Isopropylamino-5-phenyl-thiophene-2-carboxylic acid methyl ester (136 mg, 0.50 mmol) in 1,2-dichloroethane (1.0 ml) was added (1R,2S,4R)-2-Acetoxy-4-methyl-cyclohexanecarboxylic acid chloride (119 mg, 0.54 mmol) dissolved in 1,2-dichloroethane (0.6 ml) followed by PPh₃ (136 mg, 0.52 mmol). The resulting solution was stirred for 20 h at 90° C. and then cooled to room temperature. It was then diluted with ethyl acetate (10 ml) and a solution of saturated NaHCO₃ (10 ml). The aqueous phase was separated and washed with ethyl acetate (2×10 ml) and the combined organic layers were dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography (0% to 25% EtOAc/Hexane) to obtain 110 mg (45%) of (1R,2S,4R)-3-[Isopropyl-(2-Acetoxy-4-methyl-cyclohexanecarbonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester. NMR ¹H (CDCl₃, 400 MHz): 1.5:1.0 mixture of rotamers 7.73-7.70 ppm (m, 2H, H_(aro)); 7.69-7.63 ppm (m, 1H, H_(aro)); 7.51-7.41 ppm (m, 4H, H_(aro)); 7.13 ppm (s, 0.6H, H_(aro)), major rotamer); 5.79 ppm (s, 0.4H, minor rotamer); 5.21 ppm (s, 0.6H, major rotamer); 4.95-4.88 ppm (m, 1H); 3.88 ppm (s, 1.8H, major rotamer); 3.87 ppm (s, 1.2H, minor rotamer); 2.40-2.36 ppm (m, 0.6H, major rotamer); 2.11 ppm (s, 3H); 1.78-0.77 ppm (m, 16H).

Step V

(1R,2S,4R)-3-[Isopropyl-(2-Acetoxy-4-methyl-cyclohexanecarbonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid methyl ester (36 mg, 0.17 mmol) was dissolved in a mixture of dioxane:H₂O (4:1) (700 μl) and then 470 μl of LiOH 1N was added to it. After 3 h at 50° C. the reaction mixture was cooled to room temperature and the solvents were removed. The residue was then partitioned between 10 ml of H₂O acidified to pH 4 and 10 ml of EtOAc. The organic layer was separated and the aqueous phase was washed with ethyl acetate (2×10 ml). The combined ethyl acetate layers were dried (Na₂SO₄), concentrated and the residue was purified by preparative chromatography to obtain 9 mg (29%) of (1R,2S,4R)-3-[Isopropyl-(2-hydroxy-4-methyl-cyclohexanecarbonyl)-amino]-5-phenyl-thiophene-2-carboxylic acid. NMR ¹H (CDCl₃, 400 MHz): 3:2 mixture of rotamers 7.76-7.73 ppm (m, 2H, H_(aro)); 7.50-7.38 ppm (m, 3H, H_(aro)); 7.36 ppm (s, 1H, H_(aro)); 4.93-4.87 ppm (m, 1H); 4.25 ppm (s, 0.70H, major rotamer); 3.97 ppm (s, 0.3H, minor rotamer); 2.35-2.28 ppm (m, 1H); 1.99-1.53 ppm (m, 5H); 1.28 ppm (d, 0.6H, minor rotamer); 1.25 ppm (d, 1.4H, major rotamer); 1.06-1.03 ppm (m, 3H), 0.96-0.72 ppm (m, 1H); 0.79 ppm (d, 3H); 0.67-0.56 ppm (m, 1H).

Example 21 3-[(2,4-Dichloro-benzoyl)-piperidin-4-yl-amino]-5-phenyl-thiophene-2-carboxylic acid hydrochloride salt compound #368

Step I

A suspension of 3-amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (745 mg, 3.2 mmol) in dry THF (1.3 ml), at 21° C., under nitrogen, was treated with tert-butyl 4-oxo-1-piperidine carboxylate (673 mg, 3.2 mmol), followed by dibutyltin dichloride (19 mg, 0.064 mmol, 0.02 eq.). After 5 min the reaction was treated with phenyl silane (435 μL, 380 mg, 3.52 mmol, 1.1 eq). The mixture was left to stir for 74 h when a clear solution resulted. The reaction was stripped off solvent to leave a thick bright yellow gum (1.59 g). The crude material was purified by column chromatography using (CH₂Cl₂:Hexane:EtOAc)=15:5:1 as eluent to provide 4-(2-Methoxycarbonyl-5-phenyl-thiophen-3-ylamino)-piperidine-1-carboxylic acid tert-butyl ester as a yellow foam (713 mg, 54%). ¹H NMR (CDCl₃, 400 MHz) 7.63-7.60 (m, 2H), 7.74-7.36 (m, 3H), 6.90-6.84 (bs, 1H), 6.84 (s, 1H), 3.97-4.01 (m, 2H), 3.80 (s, 3H), 3.48 (bs, 1H), 3.06-2.99 (m, 2H), 2.03-1.99 (m, 2H), 1.51-1.48 (m, 2H), 1.47 (bs, 9H)

Step II

4-(2-Methoxycarbonyl-5-phenyl-thiophen-3-ylamino)-piperidine-1-carboxylic acid tert-butyl ester (200 mg, 0.48 mmol) was treated with 2.4 dichlorobenzoylchloride (202 μL, 302 mg, 1.44 mmol, 3 eq) under previously described conditions (e.g. Example 14) to provide, after column chromatography using (CH₂Cl₂:Hexane:EtOAc=15:5:1) as eluent, 4-[(2,4-Dichloro-benzoyl)-(2-methoxycarbonyl-5-phenyl-thiophen-3-yl)-amino]-piperidine-1-carboxylic acid tert-butyl ester as a pale yellow foam (165 mg, 58%), ¹H NMR (CDCl₃, 400 MHz) 7.54-7.51 (m, 2H), 7.45-7.39 (m, 3H), 7.27-7.25 (m, 2H), 7.17 (d, J=1.96 Hz, 1H), 7.06 (dd, J=1.92 Hz, J=8.34 Hz, 1H), 4.86-4.92 (m, 1H), 4.11-4.21 (m, 2H), 3.89 (s, 3H), 2.82-2.89 (m, 2H), 2.17-2.20 (m, 1H), 1.89-1.92 (m, 1H), 1.49-1.61 (m, 1H), 1.40 (bs, 9H), 1.19-1.25 (m, 1H)

Step III

A suspension of 4-[(2,4-Dichloro-benzoyl)-(2-methoxycarbonyl-5-phenyl-thiophen-3-yl)-amino]-piperidine-1-carboxylic acid tert-butyl ester (160 mg, 0.27 mmol) above in dioxane:water (4:1, 3 ml) was treated with lithium hydroxide (2M aqueous solution, 41 μL, 341 mg, 0.814 mmol, 3 eq) and the reaction allowed to stir overnight for 18 h. The reaction was stripped-off solvent and the residue partitioned between EtOAc:water (4:1). The aqueous phase was separated and extracted several times, with EtOAc, following acidification to pH 5.5 with 0.1N HCl. The combined organic extract was evaporated to a solid. The solid was taken into EtOAc and the above acid wash repeated to give, after drying and evaporation, 4-[(2-Carboxy-5-phenyl-thiophen-3-yl)-(2,4-dichloro-benzoyl)-amino]-piperidine-1-carboxylic acid tert-butyl ester as a colourless solid (128 mg, 91%), ¹H NMR (Acetone, 400 MHz) 7.75-7.70 (m, 1H), 7.64 (s, 1H), 7.52-7.40 (m, 3H), 7.52 (d, J=1.98 Hz, 1H), 7.21 (dd, J=1.96 Hz, J=8.19 Hz, 1H), 4.80-4.71 (m, 1H), 4.26-4.01 (m, 2H), 2.71-2.30 (bs, 3H), 2.25-2.17 (m, 1H), 1.82-1.69 (m, 1H), 1.40 (bs, 9H), 1.33-1.24 (m, 1H).

Step IV

A solution of 4-[(2-Carboxy-5-phenyl-thiophen-3-yl)-(2,4-dichloro-benzoyl)-amino]-piperidine-1-carboxylic acid tert-butyl ester (240 mg, 0.42 mmol) in dioxane (4 ml) at 21° C. under nitrogen was treated with anhydrous 4M HCl (3 ml, 12.6 mmol, 30 eq). After 4 h the reaction was stripped off solvent and the residue triturated with ether to give 3-[(2,4-Dichloro-benzoyl)-piperidin-4-yl-amino]-5-phenyl-thiophene-2-carboxylic acid as a pale yellow powder (214 mg, 100%) ¹H NMR (Acetone, 400 MHz) 7.76-7.73 (m, 2H), 7.64 (s, 1H), 7.45-7.38 (m, 3H), 7.30 (bs, 1H), 7.28-7.24 (m, 1H), 4.93-4.84 (m, 1H), 3.56-3.49 (m, 2H), 3.25-3.14 (m, 2H), 3.05-2.55 (bs, 1H), 2.50-2.37 (m, 2H), 2.13-1.83 (m, 1H).

Similarly prepared were Compound #366, Compound #553, Compound #543

Example 22 3-(Benzyl-cyclopropanecarbonyl-amino)-5-phenyl-thiophene-2-carboxylic acid. Compound #454

Step I

A solvent mixture of THF/MeOH/H₂O (3:2:1) was added to 3.04 g of methyl(3-amino-5-phenyl)thiophene-2-carboxylate (13 mmol) and 1.64 g of lithium hydroxide monohydrate (39 mmol). The mixture was refluxed for 8 hours and concentrated in vacuo. The crude material was taken in 100 ml of water, washed with ethyl acetate (2×100 ml) and transferred into a multineck flask. A 20% phosgene solution in toluene (11 ml, 39 mmol) was added dropwise at 0° C. A precipitate was then collected by filtration and sequentially washed by trituration with a saturated solution of bicarbonate, water, acetone and diethyl ether. 2.52 g (79%) of 6-phenyl-1H-thieno[3,2-d][1,3]oxazine-2,4-dione were isolated as a white solid. NMR ¹H (DMSO D₆, 400 MHz): 7.79-7.76 ppm (m, 2H, H_(aro)); 7.52-7.47 ppm (m, 3H, H_(aro)); 7.25 ppm (s, 1H, H_(azole)); 0.4 ppm (s, 1H, NH).

Step II

A solution of 6-phenyl-1H-thieno[3,2-d][1,3]oxazine-2,4-dione (1 g, 4.1 mmol) and anhydrous sodium carbonate (477 mg, 4.5 mmol) diluted in 15 ml of anhydrous dimethylacetamide was stirred for one hour under nitrogen before adding benzyl bromide (785 mg, 4.5 mmol). The mixture was stirred overnight at room temperature. 912 mg (66.3%) of 1-benzyl-6-phenyl-1H-thieno[3,2-d][1,3]oxazine-2,4-dione were obtained as a pale yellow solid after filtration and washing the precipitate with acetone and pentane. NMR ¹H (DMSO D₆, 400 MHz): 7.8-7.76 ppm (m, 3H, H_(aro)); 7.51-7.45 ppm (m, 3H, H_(aro)); 7.43-7.41 ppm (m, 2H, H_(aro)); 7.35-7.3 ppm (m, 2H, H_(aro)); 7.28-7.24 ppm (m, 1H, H_(aro)); 5.22 ppm (s, 1H, NCH₂).

Step III

To a solution of 1-benzyl-6-phenyl-1H-thieno[3,2-d][1,3]oxazine-2,4-dione (880 mg, 2.62 mmol) were successively added 32 ml of dioxane and 7.87 ml of NaOH 1N aqueous solution. The mixture was vigourously stirred for 2 h and then the solvents were concentrated in vacuo. Dichloromethane was added to the crude material and sodium 3-benzylamino-5-phenyl-thiophene-2-carboxylate (1.07 g, 100%) precipitated as a pale yellow solid. NMR ¹H (DMSO D₆, 400 MHz): 7.76 ppm (t, 1H, J=6.4 Hz, NH); 7.53-7.51 ppm (m, 2H, H_(aro)); 7.33-7.26 ppm (m, 6H, H_(aro)); 7.23-7.16 ppm (m, 2H, H_(aro)); 7.07 ppm (s, 1H, H_(azole)); 4.36 ppm (d, 2H, J=6.4 Hz, NHCH₂).

Step IV

To a solution of sodium 3-benzylamino-5-phenyl-thiophene-2-carboxylate (41.1 mg, 0.1 mmol) was added 32 mg (0.3 mmol) of cyclopropanecarbonyl chloride, 1.5 ml of dioxane and 0.5 ml of water. The mixture was stirred overnight at room temperature and concentrated in vacuo. A 4N hydrogen chloride solution in dioxane (1 ml) was added and the mixture was stirred for one hour at room temperature. The mixture was again concentrated and the crude material was purified by reverse phase HPLC giving access to 11.9 mg (31.5%) of 3-(benzyl-cyclopropanecarbonyl-amino)-5-phenyl-thiophene-2-carboxylic acid as a pale yellow solid. NMR ¹H (DMSO D₆, 400 MHz): 7.56-7.54 ppm (m, 2H, H_(aro))_(;) 7.39-7.13 ppm (m, 10H, H_(aro), H_(azole) and COOH); 5.27 ppm (d, 1H, J=15.2 Hz); 4.48 ppm (d, 1H, J=15.2 Hz); 1.49 ppm (m, 1H); 0.77 ppm (m, 2H); 0.61 ppm (m, 2H).

The following compounds were prepared in a similar manner: Compound #172, Compound #173, Compound #175, Compound #186, Compound #187, Compound #188, Compound #241, Compound #247, Compound #251, Compound #252, Compound #253, Compound #254, Compound #255, Compound #256, Compound #257, Compound #276, Compound #277, Compound #278, Compound #279, Compound #280, Compound #281, Compound #330, Compound #334, Compound #335, Compound #336, Compound #339, Compound #340, Compound #341, Compound #342, Compound #343, Compound #344, Compound #345, Compound #347, Compound #349, Compound #350, Compound #351, Compound #352, Compound #353 BCH-23932, Compound #354, Compound #384, Compound #385, Compound #386, Compound #388, Compound #389, Compound #390, Compound #391, Compound #392, Compound #393, Compound #394, Compound #397, Compound #398, Compound #399, Compound #400, Compound #401.

Example 23 3-[(2,4-Dichloro-phenyl)-isopropyl-carbamoyl]-5-phenyl-thiophene-2-carboxylic acid

Step I

3-Iodo-5-phenyl-thiophene-2-carboxylic acid methyl ester

A suspension of 3-Amino-5-phenyl-thiophene-2-carboxylic acid methyl ester (10 g, 43 mmol) in anhydrous benzene (200 ml), at 21° C., under N₂, was treated with t-butyl nitrite (21.8 g, 86 mmol) and the dark mixture cooled to 0° C. and treated dropwise, over 15 min, with iodine (21.8 ml, 184 mmol). After 30 min at 0° C., the solution was allowed to warm-up to ambient temperature and stirred for 2 h. The reaction mixture was then poured into water (300 ml) and stirred vigorously for 15 min. The organic phase was separated and washed several times with 20% sodium thiosulfate (4×100 ml). The resulting emulsion was filtered through celite. The celite pad was washed with EtOAc and the combined filtrate and washings were washed with more sodium thiosulfate (100 ml) to give an orange solution which was washed with brine and dried. Evaporation of the solvent afforded an oil (7.4 g). The crude oil was purified by biotage flash chromatography using Hexane/CH₂Cl₂/EtOAc (20/2/1) as eluent to give 4.42 g (29%) of 3-Iodo-5-phenyl-thiophene-2-carboxylic acid methyl ester as a pale yellow oil. NMR ¹H (CDCl₃, 400 MHz): 7.62-7.57 (m, 2H); 7.58 (s, 1H); 7.50-7.36 (m, 3H); 3.91 (s, 3H)

Step II

3-Iodo-5-phenyl-thiophene-2-carboxylic acid

A solution of 3-Iodo-5-phenyl-thiophene-2-carboxylic acid methyl ester (4.4 g, 12.78 mmol) in dioxane/water 4/1 (50 ml), at 21° C., under N₂, was treated with lithium hydroxyde (2N, 19.3 ml, 38 mmol) and the solution left to stir for 21.5 h. The reaction mixture was evaporated to dryness and the residue partitioned between EtOAc (75 ml) and water (25 ml) and acidified with 2N HCl to pH 5.5. The aqueous phase was separated and extracted with EtOAc (3×50 ml). The combined organic extract were washed with brine, dried and evaporated to give 4.12 g (97%) of 3-Iodo-5-phenyl-thiophene-2-carboxylic acid as a pale yellow solid. NMR ¹H (CD₃OD, 400 MHz): 7.69-7.67 (m, 2H); 7.55 (s, 1H); 7.46-7.39 (m, 3H).

Step III

3-Iodo-5-phenyl-thiophene-2-carboxylic acid tert-butyl ester

A suspension of magnesium sulfate (4.61 g, 38.32 mmol) in dichloromethane (37 ml) at 21° C., under N₂, was treated with conc H₂SO₄ (510 μl, 9.58 mmol). After 15 min solid 3-Iodo-5-phenyl-thiophene-2-carboxylic acid (3.7 g, 9.58 mmol) was added followed by t-butanol (4.55 ml, 47.9 mmol) and the flask was stoppered and left over-night for 19.5 h. The reaction mixture was treated with saturated bicarbonate aqueous solution, and filtered. The solid was washed with CH₂Cl₂ and the filtrate dried and concentrated to an oil. The crude material was purified by flash chromatography using Hexane/CH₂Cl₂ (3:1) as eluent to give 1.63 g (44%) of 3-Iodo-5-phenyl-thiophene-2-carboxylic acid tert-butyl ester as a colorless solid.

NMR ¹H (CDCl₃, 400 MHz) 7.61-7.59 (m, 2H); 7.43-7.35 (m, 3H), 7.25 (s, 1H), 1.60 (bs, 9H).

Step IV

3-Formyl-5-phenyl-thiophene-2-carboxylic acid tert-butyl ester

A solution of 3-Iodo-5-phenyl-thiophene-2-carboxylic acid tert-butyl ester (1.41 g, 3.65 mmol) in dry THF (37 ml) at −78° C., under nitrogen, was treated dropwise, over 5 min with n-butyl lithium (4.8 ml, 7.66 mmol). The reaction gradually darkened to a red-brown color. After 15 min at −78° C. dimethylformamide (1.7 ml, 21.9 mmol) was added dropwise over 7 min. The dark solution was allowed to stir for 2 h then quenched with saturated NH₄Cl solution (10 ml) and allowed to reach 21° C. The aqueous phase was separated and extracted with EtOAc (3×50 ml). The combined organic extracts were evaporated and the residue taken into EtOAc and washed with water, brine, dried and concentrated to give 1.14 g of a brown oil. The crude material was purified by flash chromatography using Hexane/CH2Cl2 (1/1) as eluent to provide 303 mg (28%) of 3-Formyl-5-phenyl-thiophene-2-carboxylic acid tert-butyl ester as a colorless solid. NMR ¹H: (CDCl₃, 400 MHz): 10.62 (s, 1H); 7.78 (s, 1H); 7.64-7.62 (m, 2H); 7.48-7.38 (m, 3H); 1.62 (bs, 9H).

Step V

5-Phenyl-thiophene-2,3-dicarboxylic acid 2-tert-butyl ester

A solution of 3-Formyl-5-phenyl-thiophene-2-carboxylic acid tert-butyl ester (300 mg, 1.04 mmol) in dry THF (20 ml), at 0° C., under nitrogen, was treated with methyl sulfide (10% w/w in THF, 3.8 ml, 5.2 mmol) followed by sodium dihydrogenphosphate (30% aqueous solution, 9.56 ml, 2.05 mmol). After 0.5 h, the solution was treated with sodium chlorite (30% w/w aqueous solution, 1.9 ml, 2.08 mmol) added over 1 min via a syringe. The pale yellow solution was stirred for 1.5 h at 0° C., then diluted with water (20 ml) and extracted with EtOAc (4×40 ml). The aqueous phase was separated, extracted with more EtOAc (40 ml) and the combined extracts were washed with brine dried and concentrated to give 316 mg (100%) of 5-Phenyl-thiophene-2,3-dicarboxylic acid 2-tert-butyl ester as a pale brown solid. NMR ¹H (CD₃CO; 400 MHz): 7.87 (s, 1H); 7.83-7.81 (m, 2H); 7.17-7.53 (m, 3H); 1.65 (bs, 9H).

Step VI

3-[(2,4-Dichloro-phenyl)-isopropyl-carbamoyl]-5-phenyl-thiophene-2-carboxylic acid tert-butyl ester

A solution of 5-Phenyl-thiophene-2,3-dicarboxylic acid 2-tert-butyl ester (40 mg, 0.13 mmol) in CH₂Cl₂ (1.3 ml), under nitrogen, at 0° C., was treated with diisopropylethylamine (27 L, 0.16 mmol) followed by dimethylformamide (10 L, 0.13 mmol) and oxalyl chloride (170 L, 0.34 mmol). Slight effervescence was observed. The reaction was kept at 0° C. for 30 min before being treated with (2,4-Dichloro-phenyl)-isopropyl-amine (described previously) (79 mg, 0.39 mmol). The reaction was allowed to reach 21° C. and then placed in a bath at 90° C. for 15 h. Solvent was removed to leave a pale brown gum (144 mg). The crude material was purified on bond-elute using Hexane/CH₂Cl₂/EtOAc (12.5/2/1) as eluent to give 39 mg, (62%) of 3-[(2,4-Dichloro-phenyl)-isopropyl-carbamoyl]-5-phenyl-thiophene-2-carboxylic acid tert-butyl ester as a pale brown solid. NMR ¹H (CDCl₃; 400 MHz) 7.50-7.48 (m, 2H); 7.38-7.25 (m, 6H); 7.10-7.03 (m, 1H); 5.05 (quint, J=6.88 Hz, 1H); 1.57 (bs, 9H); 1.40 (d, J=6.88 Hz, 3H); 1.12 (d, J=6.88 Hz, 3H)

Step VII 3-[(2,4-Dichloro-phenyl)-isopropyl-carbamoyl]-5-phenyl-thiophene-2-carboxylic acid

A solution of 3-[(2,4-Dichloro-phenyl)-isopropyl-carbamoyl]-5-phenyl-thiophene-2-carboxylic acid tert-butyl ester (37 mg, 0.08 mmol) in CH₂Cl₂ (0.2 ml) at room temperature, under nitrogen was treated with trifluoroacetic acid (0.8 ml). After 1 h the reaction was concentrated the residue was taken into EtOAc and washed sequentially with 2N HCl (2×15 ml), water, brine dried and evaporated to a foam (33 mg). The foam was redissolved in EtOAc and above acidic wash was repeated to yield 27 mg (84%) of 3-[(2,4-Dichloro-phenyl)-isopropyl-carbamoyl]-5-phenyl-thiophene-2-carboxylic acid compound as pale brown foam. NMR ¹H: (CD₃OD; 400 MHz) 7.57-7.55 (m, 2H); 7.49-7.36 (m, 6H), 7.30-7.27 (m, 1H); 4.89 (quint, J=6.73 Hz, 1H); 1.42 (d, J=6.73 Hz, 3H); 1.12 (d, J=6.73 Hz, 3H).

Example 24 The Following Compound was Obtained from Discovery Technology

-   3-(4-Chloro-2,5-dimethyl-benzenesulfonylamino)-5-phenyl-thiophene-2-carboxylic     acid amide Compound #580

Example 25 The Following Compounds were Obtained from Maybridge

-   5-(4-Chloro-phenyl)-3-(toluene-4-sulfonylamino)-thiophene-2-carboxylic     acid amide, Compound #563 -   5-(4-Fluoro-phenyl)-3-(toluene-4-sulfonylamino)-thiophene-2-carboxylic     acid amide, Compound #564, GK 01137 -   5-(4-Methoxy-phenyl)-3-(toluene-4-sulfonylamino)-thiophene-2-carboxylic     acid amide, Compound #565, GK 01175

Example 26 Evaluation of compounds in The HCV RNA-Dependent RNA Polymerase Assay

The following references are all incorporated by reference:

-   1. Behrens, S., Tomei, L., De Francesco, R. (1996) EMBO 15, 12-22 -   2. Harlow, E, and Lane, D. (1988) Antibodies: A Laboratory Manual.     Cold Spring Harbord Laboratory. Cold Spring Harbord. N.Y. -   3. Lohmann, V., Körner, F., Herian, U., and     Bartenschlager, R. (1997) J. Virol. 71, 8416-8428 -   4. Tomei, L., Failla, C., Santolini, E., De Francesco, R., and La     Monica, N. (1993) J Virol 67, 4017-4026

Compounds were evaluated using an in vitro polymerase assay containing purified recombinant HCV RNA-dependent RNA polymerase (NS5B protein). HCV NS5B was expressed in insect cells using a recombinant baculovirus as vector. The experimental procedures used for the cloning, expression and purification of the HCV NS5B protein are described below. Follows, are details of the RNA-dependent RNA polymerase assays used to test the compounds.

Expression of the HCV NS5B Protein in Insect Cells:

The cDNA encoding the entire NS5B protein of HCV-Bk strain, genotype 1b, was amplified by PCR using the primers NS5Nhe5′ (5′-GCTAGCGCTAGCTCAATGTCCTACACATGG-3′) and XhoNS53′ (5′-CTCGAGCTCGAGCGTCCATCGGTTGGGGAG-3′) and the plasmid pCD 3.8-9.4 as template (Tomei et al, 1993). NS5Nhe5′ and XhoNS53′ contain two NheI and XhoI sites (underlined sequences), respectively, at their 5′ end. The amplified DNA fragment was cloned in the bacterial expression plasmid pET-21b (Novagen) between the restriction sites NheI and XhoI, to generate the plasmid pET/NS5B. This plasmid was later used as template to PCR-amplify the NS5B coding region, using the primers NS5B-H9 (5′-ATACATATGGCTAGCATGTCAATGTCCTACACATGG-3′) and NS5B-R4 (5′-GGATCCGGATCCCGTTCATCGGTTGGGGAG-3′). NS5B-H9 spans a region of 15 nucleotides in the plasmid pET-21b followed by the translation initiation codon (ATG) and 8 nucleotides corresponding to the 5′ end of the NS5B coding region (nt. 7590-7607 in the HCV sequence with the accession number M58335). NS5B-R4 contains two BamHI sites (underlined) followed by 18 nucleotides corresponding to the region around the stop codon in the HCV genome (nt. 9365-9347). The amplified sequence, of 1.8 kb, was digested with NheI and BamHI and ligated to a predigested pBlueBacII plasmid (Invitrogen). The resulting recombinant plasmid was designated pBac/NS5B. Sf9 cells were co-transfected with 3 μg of pBac/NS5B, together with 1 μg of linearized baculovirus DNA (Invitrogen), as described in the manufacturer's protocol. Following two rounds of plaque purification, an NS5B-recombinant baculovirus, BacNS5B, was isolated. The presence of the recombinant NS5B protein was determined by western blot analysis (Harlow and Lane, 1988) of BacNS5B-infected Sf9 cells, using a rabbit polyclonal antiserum (anti-NS5B) raised against a His-tagged version of the NS5B protein expressed in E. coli. Infections of Sf9 cells with this plaque purified virus were performed in one-liter spinner flasks at a cell density of 1.2×10⁶ cells/ml and a multiplicity of infection of 5.

Preparation of a Soluble Recombinant NS5B Protein

Sf9 cells were infected as described above. Sixty hours post-infection, cells were harvested then washed twice with phosphate buffer saline (PBS). Total proteins were solubilized as described in Lohmann et al. (1997) with some modifications. In brief, proteins were extracted in three steps, S1, S2, S3, using lysis buffers (LB) I, LB II and LB III (Lohmann et al, 1997). The composition of LBII was modified to contain 0.1% triton X-100 and 150 mM NaCl to reduce the amount of solubilized NS5B protein at this step. In addition, sonication of cell extracts was avoided throughout the protocol to preserve the integrity of the protein structure.

Purification of Recombinant NS5B Using Fast Protein Liquid Chromatography (FPLC):

Soluble NS5B protein in the S3 fraction was diluted to lower the NaCl concentration to 300 mM, then it incubated batchwise with DEAE sepharose beads (Amersham-Pharmacia) for 2 hrs at 4° C., as described by Behrens et al. (1996). Unbound material was cleared by centrifugation for 15 min at 4° C., at 25 000 rpm using a SW41 rotor (Beckman). The supernatant was further diluted to lower the NaCl concentration to 200 mM and subsequently loaded, with a flow rate of 1 ml/min, on a 5 ml HiTrap® heparin column (Amersham-Pharmacia) connected to an FPLC® system (Amersham-Pharmacia). Bound proteins were eluted in 1 ml fractions, using a continuous NaCl gradient of 0.2 to 1 M, over a 25 ml volume. NS5B-containing fractions were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by western blotting using the anti-NS5B antiserum at a dilution of 1:2000. Positive fractions were pooled and the elution buffer was exchanged against a 50 mM NaPO₄ pH 7.0, 20% glycerol, 0.5% triton X-100 and 10 mM DTT, using a PD-10 column (Amersham-Pharmacia). The sample was then loaded onto a 1 ml HiTrap® SP column (Amersham-Pharmacia), with a flow rate of 0.1 ml/min. Bound proteins were eluted using a continuous 0 to 1 M NaCl gradient over a 15 ml volume. Eluted fractions were analyzed by SDS-PAGE and western blotting. Alternatively, proteins were visualized, following SDS-PAGE, by silver staining using the Silver Stain Plus kit (BioRad) as described by the manufacturer. Positive fractions were tested for RdRp activity (see below) and the most active ones were pooled, and stored as a 40% glycerol solution at −70° C.

In Vitro HCV RdRp Flashplate Scintillation Proximity Assay (STREP-FLASH ASSAY) Used to Evaluate Analogues:

This assay consists on measuring the incorporation of [³H] radiolabelled UTP in a polyrA/biotinylated-oligo dT template-primer, captured on the surface of streptavidin-coated scintillant-embeded microtiter Flashplates™ (NEN Life Science Products inc, MA, USA, SMP 103A). In brief, a 400 ng/μl polyrA solution (Amersham Pharmacia Biotech) was mixed volume-to-volume with 5′ biotin-oligo dT₁₅ at 20 pmol/μl. The template and primers were denatured at 95 C for 5 minutes then incubated at 37 C for 10 minutes. Annealed template-primers were subsequently diluted in a Tris-HCl containing buffer and allowed to bind to streptavidin-coated flashplates overnight. Unbound material was discarded, compounds were added in a 10 μl solution followed by a 10 μl of a solution containing 50 mM MgCl₂, 100 mM Tris-HCl pH 7.5, 250 mM NaCl and 5 mM DTT. The enzymatic reaction was initiated upon addition of a 30 μl solution containing the enzyme and substrate to obtain the following concentrations: 25 μM UTP, 1 μCi [³H] UTP and 100 nM recombinant HCV NS5B. RdRp reactions were allowed to proceed for 2 hrs at room temperature after which wells were washed three times with a 250 L of 0.15 M NaCl solution, air dried at 37 C, and counted using a liquid scintillation counter (Wallac Microbeta Trilex, Perkin-Elmer, Mass., USA). Results are shown in Table 1.

In Vitro HCV RdRp Filtration Assay Used to Evaluate Analogues

RdRp assays were conducted using the homopolymeric template/primer polyA/oligo dT. All RdRp reactions were performed in a total volume of 50 l, and in a basic buffer consisting of 20 mM Tris-HCl pH 7.5, 1 mM DTT, 50 mM NaCl, 5 mM MgCl₂, 0.5 μCi [γ³²P]-UTP (3000 Ci/mmol), 15 μM cold UTP and 20 U RNasin (Promega). Standard HCV RdRp reactions contained 200 ng of purified NS5B protein. PolyA RNAs (Amersham-Pharmacia) was resuspended at 400 ng/l. The primer oligodT₁₅ (Canadian life technologies) was diluted to a concentration of 20 μmol/l (7.6 ng/ml). Templates and primers were mixed volume to volume, denatured at 95° C. for 5 min and annealed at 37° C. for 10 min. Following a two hour incubation at 22° C., reactions were stopped by the addition of 100 μg of sonicated salmon sperm DNA (Life Technologies) and 1 ml of 10% trichloroacetic acid-0.5% tetrasodium pyrophosphate (TCA-PPi). Nucleic acids were precipitated at 4° C. for 30 min after which samples were filtered on GF/C glass microfiber filters (Millipore). Membranes were subsequently washed with 25 ml of a 1% TCA-0.1% PPi solution, then air dried. Incorporated radioactivity was quantified using a liquid scintillation counter (1450-Microbeta, Wallac). Results are shown in Table 1.

Example 27 Evaluation of Analogues for measurement of ATPase activity of HCV NS3 helicase

Malachite Green Assay:

The measurement of ATPase activity was performed by measuring the amount of free inorganic phosphate released during the conversion of ATP to ADP by the HCV NS3 ATPase activity. The assay is as follows: In a 96-well microtiter-plate, compounds were dissolved at various concentrations in a final volume of 25 μL of ATPase buffer containing 400 μM ATP. The enzymatic reaction was initiated by the addition of 25 μl of ATPase buffer containing 6 nM of HCV NS3 enzyme without ATP to the wells followed by an incubation of 30 min. at 37 C. Essentially, the final concentration of the ATPase buffer components are as follows: 44 mM MOPS pH 7.0, 8.8 mM NaCl, 2.2 mM MgCl₂, 125 μg/ml poly A, 1% DMSO, 200 μM ATP, and 3 nM HCV NS3 enzyme. The reaction was stopped by the addition of 100 μl of Biomol Green™ reagent (BIOMOL® Research Laboratories Inc., Plymouth Meeting, Pa.). In order to allow the development of the green color, the plate was incubated for 15 min. at room temperature. Then the plate was read on a micro-plate reader at 620 nm. The 50% inhibitory concentration (IC₅₀) for anti-ATPase activity was defined as the concentration of compound that resulted in a 50% reduction of the signal compared to the signal observed in control sample without compound. The signal recorded was also corrected from the background signal obtained with control samples with compound only. The IC₅₀ was determined from dose-response curves using six to eight concentrations per compound. Curves were fitted to data points using a non-linear regression analysis, and IC₅₀s were interpolated from the resulting curves using GraphPad Prism software, version 2.0 (GraphPad Software Inc, San Diego, Calif.).

HPLC Assay:

The measurement of HCV NS3 ATPase activity was performed by measuring the amount of ADP produced during the conversion of ATP to ADP by the HCV NS3 enzyme using paired-ion HPLC on a reverse phase column. The assay is as follows: The same protocol as mentioned above was used except that the final concentration of HCV NS3 enzyme was reduced to 1 nM in a 50 μl reaction mixture and that the ATPase reaction was stopped by the addition of 12.5 μl of 0.5 M EDTA. A modular liquid chromatography system (TSP Spectrasystem®, ThermoQuest Corporation, San Diego, USA) using a ChromQuest™ software (ThermoQuest Corporation, San Diego, USA) controlled the autosampling of 25 μl from each reaction. The mobile phase was an isocratic solution of 0.15 M triethylamine, 6% methanol, and phosphoric acid to pH 5.5. ADP and ATP peaks were resolved using the Aqua 5μ, C18, 125 Å, (150×4.6 mm) reverse phase column. The extent of ATP conversion to ADP was evaluated by measuring the area under the ADP peak produced which was detected at 259 nm. The amount of ADP was corrected for the presence of ADP contaminant in the original ATP solution. The 50% inhibitory concentration (IC₅₀) for anti-ATPase activity was defined as the concentration of compound that resulted in a 50% reduction of the ADP peak area compared to the ADP peak area observed in control sample without compound. The IC₅₀ was determined from dose-response curves using six to eight concentrations per compound. Curves were fitted to data points using a non-linear regression analysis, and IC₅₀s were interpolated from the resulting curves using GraphPad Prism software, version 2.0 (GraphPad Software Inc, San Diego, Calif.).

Example 27 List of Compounds and Related Polymerase Activity *

MOLSTRUCTURE COMPOUND NAME IC₅₀  1

3-[(4-CHLORO-2,5-DIMETHYL- BENZENESULFONYL)-(3-IODO- BENZYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++  2

3-[(3-BENZOFURAN-2-YL-BENZYL)-(4- CHLORO-2,5-DIMETHYL- BENZENESULFONYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  3

3-(4-CHLORO-2,5-DIMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  4

3-{(2,4-DICHLORO-BENZOYL)-[5-(3- TRIFLUOROMETHYL-PHENYL)- FURAN-2-YLMETHYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  5

3-[(4-CHLORO-2,5-DIMETHYL- BENZENESULFONYL)-METHYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++  6

5-(4-FLUORO-PHENYL)-3-(TOLUENE-4- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  7

3-(2,4-DICHLORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  8

3-(4-CHLORO-2,5-DIMETHYL- BENZENESULFONYEAMINO)-5-(4- FLUORO-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++  9

3-[(2,4-DICHLORO-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++  10

5-#TERT!-BUTYL-3-(4-CHLORO- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++  11

4-(TOLUENE-4-SULFONYLAMINO)- [2,3′]BITHIOPHENYL-5-CARBOXYLIC ACID ++  12

3-[(5-BENZOFURAN-2-YL-THIOPHEN-2- YLMETHYL)-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++  13

5-PHENYL-3-(TOLUENE-4- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  14

3-(4-CHLORO-2,5-DIMETHYL- BENZENESULFONYLAMINO)-5-(4- CHLORO-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++  15

5-PHENYL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +  16

5-PHENYL-3-(TOLUENE-3- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++  17

3-BENZENESULFONYLAMINO-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  18

3-(4-CHLORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  19

3-(4-CHLORO-2,5-DIMETHYL- BENZENESULFONYLAMINO)-5-(4- ISOBUTYL-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID ++  20

5-TERT-BUTYL-3-(4-CHLORO-2,5- DIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++  21

3-(2,5-DIMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  22

3-(4-METHOXY-2,3,6-TRIMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  23

5-PHENYL-3-(THIOPHENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  24

4-(4-CHLORO-2,5-DIMETHYL- BENZENESULFONYLAMINO)- [2,3′]BITHIOPHENYL-5-CARBOXYLIC ACID +++  25

5-(3,5-BIS-TRIFLUOROMETHYL- PHENYL)-3-(4-CHLORO-2,5- DIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +  26

8-CHLORO-3-(4-CHLORO-2,5- DIMETHYL- BENZENESULFONYLAMINO)-4#H!-1,5- DITHIA- CYCLOPENTA[#A!]NAPHTHALENE-2- CARBOXYLIC ACID ++  27

3-(2,4-DIFLUORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  28

3-[3-(2,6-DICHLORO-PYRIDIN-4-YL)- UREIDO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++  29

3-(2-CHLORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  30

3-(2-FLUORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  31

5-PHENYL-3-(2-TRIFLUOROMETHOXY- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +  32

3-(4-#TERT!-BUTYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  33

3-(4-CHLORO- PHENOXYCARBONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  34

3-(3,4-DICHLORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  35

5-PHENYL-3-(2-TRIFLUOROMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +  36

3-(5-BROMO-6-CHLORO-PYRIDINE-3- SULFONYLAMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +  37

3-(5-CHLORO-THIOPHENE-2- SULFONYLAMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++  38

3-(5-CHLORO-3-METHYL- BENZO[#B!]THIOPHENE-2- SULFONYLAMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++  39

3-(4-BROMO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  40

3-(3-CHLORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  41

3-(5-CHLORO-1,3-DIMETHYL-1#H!- PYRAZOLE-4-SULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  42

3-(3-BROMO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  43

3-(4-ISOPROPYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  44

3-(2,6-DICHLORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  45

3-(2-NITRO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  46

5-PHENYL-3-(5-[1,2,3]THIADIAZOL-4- YL-THIOPHENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++  47

5-PHENYL-3-(PYRIDINE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +  48

3-(2,4-DICHLORO-BENZYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  49

3-(3-FLUORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  50

5-PHENYL-3-(3-TRIFLUOROMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++  51

3-(2-CARBOXY-BENZOYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID METHYL ESTER ++  52

5-PHENYL-3-(4-TRIFLUOROMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++  53

3-(2,5-DIFLUORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  54

3-(2-CYANO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  55

3-(2,5-DICHLORO-THIOPHENE-3- SULFONYLAMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++  56

4-(TOLUENE-2-SULFONYLAMINO)- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID +++  57

5′-CHLORO-4-(TOLUENE-2- SULFONYLAMINO)- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID +++  58

5-(2,4-DICHLORO-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++  59

5-(4-NITRO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  60

3-(TOLUENE-2-SULFONYLAMINO)-5- (4-TRIFLUOROMETHOXY-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++  61

5-QUINOLIN-8-YL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +  62

5-PHENYL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  63

5-(3-NITRO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  64

3-(TOLUENE-2-SULFONYLAMINO)-5- M-TOLYL-THIOPHENE-2-CARBOXYLIC ACID +++  65

5-(3-CHLORO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  66

5-(4-FLUORO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  67

5-(3-FLUORO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  68

5-(4-CHLORO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  69

5-(3,5-DIFLUORO-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++  70

5-(3,4-DIFLUORO-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++  71

3-(TOLUENE-2-SULFONYLAMINO)-5- VINYL-THIOPHENE-2-CARBOXYLIC ACID ++  72

3-(4-CHLORO-BENZOYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  73

3-[(4-CHLORO-BENZOYL)-METHYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++  74

5-PHENYL-3-[(THIOPHENE-2- CARBONYL)-AMINO]-THIOPHENE-2- CARBOXYLIC ACID ++  75

3-[METHYL-(THIOPHENE-2- CARBONYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++  76

3-(2-BROMO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++  77

3-(2,4-DIFLUORO-BENZOYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +  78

3-[(2,4-DIFLUORO-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++  79

3-(TOLUENE-2-SULFONYLAMINO)-5- TRIMETHYLSILANYLETHYNYL- THIOPHENE-2-CARBOXYLIC ACID +++  80

5-ETHYNYL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++  81

3-(TOLUENE-2-SULFONYLAMINO)-5- (3-TRIFLUOROMETHOXY-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID ++  82

5-BENZOYL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++  83

5-(4-CYANO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  84

5-(3-CHLORO-4-FLUORO-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++  85

5-(3,4-DICHLORO-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++  86

5-PYRIDIN-4-YL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  87

5-PYRIDIN-3-YL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++  88

3-(TOLUENE-2-SULFONYLAMINO)-5- (4-TRIFLUOROMETHYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID) +++  89

5-(4-METHANESULFONYL-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++  90

5-(3-ACETYLAMINO-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++  91

5-(3-CHLORO-4-FLUORO-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++  92

3-(4-METHYL-BENZOYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  93

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++  94

3-(3,5-DIMETHYL-ISOXAZOLE-4- SULFONYLAMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++  95

3-[(2-CHLORO-BENZOYL)-METHYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++  96

3-(2-METHYL-BENZOYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++  97

3-[METHYL-(2-METHYL-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +  98

5-PHENYL-3-(5-TRIFLUOROMETHYL- PYRIDINE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++  99

5-PHENYLETHYNYL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 100

3-(2,5-DIMETHYL- BENZENESULFONYLAMINO)-5-(4- NITRO-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++ 101

5-(2-FLUORO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 102

5-(2-CYANO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 103

5-(2-ETHOXYCARBONYL-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++ 104

5-(2-METHOXY-PHENYL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 105

3′-METHYL-4-(TOLUENE-2- SULFONYLAMINO)- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID ++ 106

3-(TOLUENE-2-SULFONYLAMINO)-5- (2-TRIFLUOROMETHYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID ++ 107

3-(2,5-DIMETHYL- BENZENESULFONYLAMINO)-5-(4- FLUORO-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++ 108

5-STYRYL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 109

3-(2,4-DIFLUORO- BENZENESULFONYLAMINO)-5-(4- NITRO-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++ 110

3-(2,4-DIFLUORO- BENZENESULFONYLAMINO)-5-(4- FLUORO-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++ 111

3-[[5-(3-CHLORO-4-FLUORO-PHENYL)- THIOPHEN-2-YLMETHYL]-(2,4- DICHLORO-BENZOYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 112

3-[(4-OXO-1-PHENYL-1,3,8-TRIAZA- SPIRO[4.5]DECANE-8-CARBONYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 113

3-{[4-(2-OXO-2,3-DIHYDRO- BENZOIMIDAZOL-1-YL)-PIPERIDINE-1- CARBONYL]-AMINO}-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID + 114

3-{[4-(4-NITRO-PHENYL)-PIPERAZINE- 1-CARBONYL]-AMINO}-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 115

5-(2-CARBOXY-PHENYL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 116

5-(4-CHLORO-PHENYL)-3-(PYRIDINE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 117

5-(3-CYANO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 118

3-(2,5-DIMETHYL- BENZENESULFONYLAMINO)-5-P- TOLYL-THIOPHENE-2-CARBOXYLIC ACID +++ 119

3-(2,4-DIFLUORO- BENZENESULFONYLAMINO)-5-P- TOLYL-THIOPHENE-2-CARBOXYLIC ACID +++ 120

5-PHENETHYL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 121

5-(3-ETHOXYCARBONYL-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++ 122

5-(4-METHOXY-PHENYL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 123

5-(3-METHOXY-PHENYL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 124

5-(4′-BROMO-BIPHENYL-4-YL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 125

5-(4-HYDROXYMETHYL-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 126

5-FURAN-3-YL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 127

5-BENZOFURAN-2-YL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 128

5-PYRIDIN-2-YL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 129

5-(4-NITRO-PHENYL)-3-(PYRIDINE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 130

3-[(BENZOFURAN-2-CARBONYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID + 131

3-[(2,4-DIMETHYL-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 132

3-[[5-(2-CYANO-PHENYL)-THIOPHEN- 2-YLMETHYL]-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 133

5-(4-FLUORO-PHENYL)-3-(PYRIDINE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 134

5-[2-(4-CHLORO-PHENYL)-VINYL]-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++ 135

3-BENZENESULFONYLAMINO-5-(4- FLUORO-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++ 136

3-(2,4-DIMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 137

5-PHENYL-3-(2-VINYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++ 138

3-(4-BROMO-2,5-DIFLUORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 139

3-(2-ACETYLAMINO-4-METHYL THIAZOLE-5-SULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 140

3-(4-ACETYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 141

3-(4-FLUORO-2-TRIFLUOROMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 142

3-(2-METHOXY-4-METHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 143

3-(3,4-DIFLUORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 144

4-(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YLSULFAMOYL)-5-(4-CHLORO- PHENYL)-2-METHYL-FURAN-3- CARBOXYLIC ACID ETHYL ESTER ++ 145

3-(4-FLUORO-3-TRIFLUOROMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 146

3-(2-AMINO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 147

3-(3-NITRO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 148

3-(4-NITRO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 149

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 150

5-(3-CYANO-BENZYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID + 151

5-PHENYL-3-(2,4,6-TRIFLUORO- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 152

3-(4-METHOXY-2-NITRO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 153

5-PHENYL-3-(2,3,4-TRICHLORO- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 154

5-(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YLSULFAMOYL)-2-METHYL-FURAN- 3-CARBOXYLIC ACID METHYL ESTER +++ 155

4-(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YLSULFAMOYL)-2-METHYL-1,5- DIPHENYL-1H-PYRROLE-3- CARBOXYLIC ACID ETHYL ESTER +++ 156

5-PHENYL-3-{[4-(3- TRIFLUOROMETHYL-PHENYL)- PIPERAZINE-1-CARBONYL]-AMIN}- THIOPHENE-2-CARBOXYLIC ACID ++ 157

3-{[4-(4-FLUORO-PHENYL)- PIPERAZINE-1-CARBONYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID + 158

3-{[4-(2,6-DIMETHYL-PHENYL)- PIPERAZINE-1-CARBONYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID + 159

3-{[4-(2-CHLORO-PHENYL)- PIPERAZINE-1-CARBONYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 160

3-{[4-(3-CHLORO-PHENYL)- PIPERAZINE-1-CARBONYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 161

4,4′-BIS-(TOLUENE-2- SULFONYLAMINO)- [2,2′]BITHIOPHENYL-5,5′- DICARBOXYLIC ACID +++ 162

3-[ALLYL-(4-METHYL-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 163

5-(1-DIMETHYLSULFAMOYL-1#H!- PYRAZOL-4-YL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 164

5-(3-AMINO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 165

5-(4-AMINO-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 166

5-(4-ACETYL-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 167

4-(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YLSULFAMOYL)-2,5-DIMETHYL-1H- PYRROLE-3-CARBOXYLIC ACID ETHYL ESTER ++ 168

4-(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YLSULFAMOYL)-5-(4-CHLORO- PHENYL)-3-METHYL-1-PHENYL-1H- PYRROLE-2-CARBOXYLIC ACID ETHYL ESTER ++ 169

3-(3,5-DICHLORO-4-HYDROXY- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 170

5-(1#H!-PYRAZOL-4-YL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 171

5-(3-HYDROXY-PHENYL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 172

3-[METHYL-(3-METHYL-BUTYRYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 173

3-{[2-(4-FLUORO-PHENYL)-ACETYL]- METHYL-AMINO}-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 174

3-(4-PENTYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 175

3-(METHYL-PHENYLACETYL-AMINO)- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 176

3-[2,5-BIS-(2,2,2-TRIFLUORO-ETHOXY)- BENZENESULFONYLAMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 177

3-(4-METHYL-2-NITRO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 178

5-THIAZOL-2-YL-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 179

5-PHENYL-3-[3-(3-PHENYL-PROPYL)- UREIDO]-THIOPHENE-2-CARBOXYLIC ACID ++ 180

3-[(3,4-DIHYDRO-1H-ISOQUINOLINE-2- CARBONYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 181

3-{[4-(4-METHOXY-PHENYL)- PIPERAZINE-1-CARBONYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 182

3-{[4-(6-METHYL-PYRIDIN-2-YL)- PIPERAZINE-1-CARBONYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID HYDROCHLORIDE ++ 183

3-{[4-(4-CHLORO-BENZYL)- PIPERAZINE-1-CARBONYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID HYDROCHLORIDE ++ 184

5-(5-METHYL-PYRIDIN-2-YL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 185

3-[ETHYL-(4-METHYL-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 186

3-[(3-CHLORO-THIOPHENE-2- CARBONYL)-METHYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 187

3-[(2-BROMO-BENZOYL)-METHYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 188

3-[(4-BUTYL-BENZOYL)-METHYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID + 189

3-(2-CHLOROMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 190

5-(4-HYDROXY-PHENYL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 191

5-(5-CHLORO-PYRIDIN-2-YL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 192

5-(4-CHLORO-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID ++ 193

5-(4-CYANO-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID ++ 194

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-(4-NITRO-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID ++ 195

5-(4-HYDROXYMETHYL-PHENYL)-3- [METHYL-(4-METHYL-BENZOYL)- AMINO]-THIOPHENE-2-CARBOXYLIC ACID ++ 196

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-(3-NITRO-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++ 197

5-(4-FLUORO-PHENYL)-3-[METHYL-(4 METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 198

5-(4-METHOXY-PHENYL)-3-[METHYL- (4-METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID ++ 199

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-#P!-TOLYL-THIOPHENE-2- CARBOXYLIC ACID ++ 200

5-(4-AMINO-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID ++ 201

3-[CYCLOPENTYL-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 202

5-BENZO[1,3]DIOXOL-5-YL-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 203

3-[(2-HYDROXY-ETHYL)-(4-METHYL- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 204

3-[(2,4-DICHLORO-BENZOYL)- ISOBUTYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 205

3-[(2-METHOXY-4-METHYL- BENZOYL)-METHYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 206

5-(3-CYANO-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 207

5-(2-CHLORO-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID ++ 208

3-[(2,4-DICHLORO-BENZOYL)- PHENYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 209

3-[4-(TRIFLUOROMETHYL- BENZOYL)METHYLAMINE]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 210

3-[(4-CHLORO-BENZOYL)-ISOPROPYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 211

3-[ISOPROPYL-(4-METHYL-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 212

5-(3,5-DIFLUORO-PHENYL)-3- [METHYL-(4-METHYL-BENZOYL)- AMINO]-THIOPHENE-2-CARBOXYLIC ACID +++ 213

5-(3-FLUORO-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 214

5-(2,4-DIFLUORO-PHENYL)-3- [METHYL-(4-METHYL-BENZOYL)- AMINO]-THIOPHENE-2-CARBOXYLIC ACID ++ 215

5-(4-HYDROXY-PHENYL)-3-[METHYL- (4-METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 216

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-(4-TRIFLUOROMETHOXY- PHENYL)-THIOPHENE-2- CARBOXYLIC ACID ++ 217

5-(2-HYDROXY-PHENYL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 218

3-[(2-CHLORO-BENZOYL)-ISOPROPYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 219

3-[(3,5-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 220

3-(4-BROMO-2-METHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 221

3-(5-CARBOXY-4-CHLORO-2-FLUORO- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID + 222

5-PHENYL-3-(2,3,4-TRIFLUORO- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++ 223

3-(4-BROMO-2-FLUORO- BENZENESULFONYLAMINO)-5-(4- ISOBUTYL-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID ++ 224

3-(4-BROMO-2-METHYL- BENZENESULFONYLAMINO)-5-(4- ISOBUTYL-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID + 225

5-(4-ISOBUTYL-PHENYL)-3-(3- METHOXY- BENZENESULJFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID + 226

3-[(4-FLUORO-BENZOYL)-ISOPROPYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 227

3-[2,5-BIS-(2,2,2-TRIFLUORO-ETHOXY)- BENZENESULFONYLAMINO]-5-(4- ISOBUTYL-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID + 228

3-(2-CHLORO-4-CYANO- BENZENESULFONYLAMINO)-5-(4- ISOBUTYL-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID + 229

5′-ACETYL-4-(TOLUENE-2- SULFONYLAMINO)- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID +++ 230

5-BENZO[B]THIOPHEN-2-YL-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 231

5-(4-BUTYL-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 232

5-(4-ETHYL-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 233

3-[BENZYL-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 234

3-[(4-CHLORO-2-METHYL-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 235

3-[(2,4-DIMETHYL- BENZENESULFONYL)-METHYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 236

5-(4-ACETYL-PHENYL)-3-(2,4- DIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 237

5-(4-ACETYL-PHENYL)-3-(TOLUENE-4- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 238

5-(4-ACETYL-PHENYL)-3-(4-CHLORO- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 239

5-(4-CARBOXY-PHENYL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID #TERT!-BUTYL ESTER ++ 240

3-[(2,4-DIMETHYL- BENZENESULFONYL)-ISOPROPYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 241

3-[ACETYL-(4-CHLORO-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 242

3-ETHANESULFONYLAMINO-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 243

3-[ISOPROPYL-(4- TRIFLUOROMETHYL-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 244

3-[(2,4-DICHLORO-BENZOYL)-(3- METHYL-BUT-2-ENYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 245

3-[(2,6-DICHLORO-PYRIDINE-3- CARBONYL)-METHYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 246

3-[(6-CHLORO-PYRIDINE-3- CARBONYL)-ISOPROPYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID + 247

3-[(4-TERT-BUTYL-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 248

5-(4-CARBOXY-PHENYL)-3-(TOLUENE- 2-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 249

5-(4-ETHOXY-PHENYL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 250

3-[(2,6-DICHLORO-PYRIDINE-3- CARBONYL)-ISOPROPYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 251

3-[(BENZO[B]THIOPHENE-2- CARBONYL)-METHYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 252

3-[METHYL-(NAPHTHALENE-2- CARBONYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 253

3-[(3,4-DICHLORO-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 254

3-[(3,5-DICHLORO-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 255

3-[(4-BROMO-3-METHYL-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 256

3-[(3-CHLORO-BENZO[B]THIOPHENE- 2-CARBONYL)-METHYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 257

3-[METHYL-(4-METHYL-3-NITRO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 258

5-(4-CARBAMOYL-PHENYL)-3-(2,4- DIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++ 259

5-(4-CARBAMOYL-PHENYL)-3- (TOLUENE-4-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 260

5-(1H-INDOL-5-YL)-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 261

3-[#SEC!-BUTYL-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 262

3-[(2,4-DIMETHYL-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 263

5-(4-AZIDO-PHENYL]-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID +++ 264

3-[(2,4-DICHLORO-BENZOYL)-(1- PHENYL-ETHYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 265

5-(4-CARBAMOYL-PHENYL)-3-(4- CHLORO- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 266

5-(2-FLUORO-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 267

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-#O!-TOLYL-THIOPHENE-2- CARBOXYLIC ACID +++ 268

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-M-TOLYL-THIOPHENE-2- CARBOXYLIC ACID +++ 269

5-(3-CHLORO-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 270

5-(3,4-DIFLUORO-PHENYL)-3- [METHYL-(4-METHYL-BENZOYL)- AMINO]-THIOPHENE-2-CARBOXYLIC ACID +++ 271

5-(3-AMINO-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID ++ 272

5-(3-ACETYL-PHENYL)-3-[METHYL-(4- METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID ++ 273

5-(3-HYDROXY-PHENYL-3-[METHYL- (4-METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 274

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-(3-TRIFLUOROMETHYL- PHENYL)-THIOPHENE-2-CARBOXYLIC ACID +++ 275

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-(4-TRIFLUOROMETHYL- PHENYL)-THIOPHENE-2-CARBOXYLIC ACID +++ 276

3-[(3,4-DIMETHOXY-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID + 277

3-[METHYL-(2,4,6-TRIFLUORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 278

3-[(2,3-DIFLUORO-4- TRIFLUOROMETHYL-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 279

3-[(3-FLUORO-4-TRIFLUOROMETHYL- BENZOYL)-METHYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 280

3-[(2,3-DIFLUORO-4-METHYL- BENZOYL)-METHYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 281

3-[(2-FLUORO-4-TRIFLUOROMETHYL- BENZOYL)-METHYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 282

5-(4-CARBAMOYL-PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++ 283

5-(4-FLUORO-PHENYL)-3-[ISOPROPYL- (4-METHYL-BENZOYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 284

3-[(2-BROMO-4-CHLORO-BENZOYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 285

3-(2,6-DIMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 286

3-[METHYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 287

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID METHYL ESTER ++ 288

5-(4-CYANO-PHENYL)-3-(2,4- DIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 289

3-(4-CHLORO- BENZENESULFONYLAMINO)-5-(4- CYANO-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++ 290

5-(4-CYANO-PHENYL)-3-(TOLUENE-4- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 291

5′-ACETYL-4-(2,4-DIMETHYL- BENZENESULFONYLAMINO)- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID +++ 292

5′-ACETYL-4-(2,6-DIMETHYL- BENZENESULFONYLAMINO)- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID +++ 293

3-[METHYL-(4-METHYL-THIOPHENE-2- CARBONYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 294

5-(3-CHLORO-PHENYL)-3-[(2,4- DICHLORO-BENZOYL)-ISOPROPYL- AMINO]-THIOPHENE-2-CARBOXYLIC ACID +++ 295

5′-CYANO-4-(TOLUENE-2- SULFONYLAMINO)- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID +++ 296

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-PYRIDIN-2-YL-THIOPHENE- 2-CARBOXYLIC ACID ++ 297

3-[(2,4-DICHLORO-THIOBENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 298

5-PHENYL-3-(2,4,6-TRIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 299

3-[(1-CARBOXY-ETHYL)-(2,4- DICHLORO-BENZOYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 300

3-[(4-METHYL-BENZOYL)-(3-METHYL- BUT-2-ENYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 301

3-[(2-HYDROXY-4-METHYL- BENZOYL)-ISOPROPYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 302

3-[METHYL-(4-METHYL-BENZOYL)- AMINO]-5-PYRIDIN-3-YL-THIOPHENE- 2-CARBOXYLIC ACID ++ 303

5′-ACETYL-4-[METHYL-(4-METHYL- BENZOYL)-AMINO]- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID +++ 304

3-[ISOPROPYL-(4-METHYL-BENZOYL)- AMINO]-5-(3-TRIFLUOROMETHYL- PHENYL)-THIOPHENE-2-CARBOXYLIC ACID +++ 305

3-[ISOPROPYL-(4-METHYL-BENZOYL)- AMINO]-5-M-TOLYL-THIOPHENE-2- CARBOXYLIC ACID +++ 306

3-[(2-BROMO-4-CHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 307

3-[(4-CHLORO-2-FLUORO-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 308

3-(2,4-DIMETHYL- BENZENESULFONYLAMINO)-4- METHYL-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 309

3-[(2-BROMO-4-METHYL-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 310

3-[(4-CHLORO-2-IODO-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 311

3-[(4-CYANO-BENZOYL)-ISOPROPYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 312

3-[ALLYL-(4-METHYL-BENZOYL)- AMINO]-5-[4-(2-CARBOXY-VINYL)- PHENYL]-THIOPHENE-2-CARBOXYLIC ACID. +++ 313

3-[(4-CHLORO-2-HYDROXY- BENZOYL)-ISOPROPYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 314

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-4-METHYL-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 315

5-#TERT!-BUTYL-3-(2,4-DIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID ++ 316

3-[ISOPROPYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID + 317

3-[ISOPROPYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 318

5-[4-(2-CARBOXY-ETHYL)-PHENYL]-3- [(4-METHYL-BENZOYL)-PROPYL- AMINO]-THIOPHENE-2-CARBOXYLIC ACID ++ 319

5-BENZOFURAN-2-YL-3-(2,4- DIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 320

3-(2,4-DIMETHYL- BENZENESULFONYLAMINO)-5-(4- HYDROXYMETHYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++ 321

3-(2,4-DIMETHYL- BENZENESULFONYLAMINO)-5-(4- METHANESULFONYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++ 322

5-[4-(2-CARBOXY-VINYL)-PHENYL]-3- (2,4-DIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2-CARBOXYLIC ACID +++ 323

3-[ALLYL-(4-METHYL-BENZOYL)- AMINO-5-[3-(2-CARBOXY-VINYL)- PHENYL]-THIOPHENE-2-CARBOXYLIC ACID ++ 324

3-[ISOPROPYL-(2,4,6-TRIMETHYL- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 325

5-[3-(2-CARBOXY-ETHYL)-PHENYL]-3- [(4-METHYL-BENZOYL)-PROPYL- AMINO]-THIOPHENE-2-CARBOXYLIC ACID ++ 326

3-[(2-FLUORO-4-TRIFLUOROMETHYL- BENZOYL)-ISOPROPYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 327

3-[#TERT!-BUTYL-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 328

3-[(2-AMINO-4-CHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 329

3-[(4-CHLORO-2-NITRO-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 330

3-[(4-METHYL-BENZOYL)-(3- TRIFLUOROMETHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 331

3-[(3-FLUORO-4-METHYL-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 332

5-(4-CARBOXY-PHENYL)-3-(2,4- DIMETHYL- BENZENESULFONYLAMINO)- THIOPHENE-2- CARBOXYLIC ACID +++ 333

3-[CYCLOPROPYL-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 334

3-[(3-TERT-BUTYL-BENZYL)-(4- METHYL-BENZOYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 335

3-[(3-CHLORO-BENZYL)-(4-METHYL- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 336

3-[(2,4-DIFLUORO-BENZYL)-(4- METHYL-BENZOYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 337

3-[(4-CHLORO-2,5-DIFLUORO- BENZOYL)-ISOPROPYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 338

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-(2-METHYL- ALLYL)-THIOPHEN E-2-CARBOXYLIC ACID +++ 339

3-{ALLYL-[2-(4-CHLORO-PHENYL) ACETYL]-AMINO}-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 340

3-[BENZYL-(4-METHYL-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 341

3-[(4-CHLORO-BENZYL)-(4-METHYL- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 342

3-[(4-METHYL-BENZOYL)-(4-NITRO- BENZYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 343

3-[(4-METHYL-BENZOYL)-(2-METHYL- BENZYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 344

3-[(3-METHOXY-BENZYL)-(4-METHYL- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 345

3-[(2-CHLORO-BENZYL)-(4-METHYL- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 346

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-ISOBUTYL- THIOPHENE-2-CARBOXYLIC ACID +++ 347

3-[ALLYL-(2-NAPHTHALEN-2-YL- ACETYL)-AMINO-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID + 348

3-{ALLYL-[2-(2,4-DICHLORO-PHENYL)- ACETYL]-AMINO}-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 349

3-{ALLYL-[2-(2-CHLORO-4-FLUORO- PHENYL)-ACETYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 350

3-{ALLYL-[2-(3,4-DICHLORO-PHENYL)- ACETYL]-AMINO}-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 351

3-{ALLYL-[2-(2,4-DIFLUORO-PHENYL)- ACETYL]-AMINO}-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 352

3-{ALLYL-[2-(4-TRIFLUOROMETHYL- PHENYL)-ACETYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID + 353

3-{ALLYL-[2-(2,6-DICHLORO-PHENYL)- ACETYL]-AMINO}-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 354

3-[ALLYL-(2-M-TOLYL-ACETYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 355

5-(4-ACETYL-PHENYL)-3-[(2,4- DICHLORO-BENZOYL)-ISOPROPYL- AMINO]-THIOPHENE-2-CARBOXYLIC ACID +++ 356

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-(4-FLUORO- PHENYL)-THIOPHENE-2-CARBOXYLIC ACID +++ 357

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-M-TOLYL- THIOPHENE-2-CARBOXYLIC ACID +++ 358

5′-ACETYL-4-[(2,4-DICHLORO- BENZOYL)-ISOPROPYL-AMINO]- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID +++ 359

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO-5-(3- TRIFLUOROMETHYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++ 360

4-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5′-METHYL- [2,2′]BITHIOPHENYL-5-CARBOXYLIC ACID +++ 361

3-(2,4-DIMETHYL- BENZENESULFONYLAMINO)-5-(4- METHOXY-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++ 362

3-(CYCLOHEXANECARBONYL- ISOPROPYL-AMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 363

3-{(2,4-DICHLORO-BENZOYL)-[1-(2,4- DICHLORO-BENZOYL)-PIPERIDIN-4- YL]-AMINO}-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 364

4-[(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YL)-(4-METHYL-BENZOYL)-AMINO]- PIPERIDINE-1-CARBOXYLIC ACID #TERT!-BUTYL ESTER +++ 365

4-[(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YL)-(2,4-DICHLORO-BENZOYL)- AMINO]-PIPERIDINE-1-CARBOXYLIC ACID #TERT!-BUTYL ESTER +++ 366

3-[(4-METHYL-BENZOYL)-PIPERIDIN- 4-YL-AMINO]-5-PHENYL-THIOPHENE- 2-CARBOXYLIC ACID +++ 367

5′-ACETYL-4-(2,4-DIMETHYL- BENZENESULFONYLAMINO)- [2,3′]BITHIOPHENYL-5-CARBOXYLIC ACID +++ 368

3-[(2,4-DICHLORO-BENZOYL)- PIPERIDIN-4-YL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 369

5-(4-METHANESULFONYLAMINO- PHENYL)-3- (TOLUENE-2-SULFONYLAMINO)- THIOPHEN E-2-CARBOXYLIC ACID ++ 370

3-(4-FLUORO-2-METHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 371

3-[(3-METHYL- CYCLOHEXANECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 372

3-(4-CHLORO-2-METHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 373

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO-5-(4- METHANESULFONYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++ 374

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-(4- METHANESULFONYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++ 375

5-(4-CARBOXY-PHENYL)-3-[(2,4- DICHLORO-BENZOYL)-ISOPROPYL- AMINO]-THIOPHENE-2-CARBOXYLIC ACID +++ 376

5-BENZOFURAN-2-YL-3-[(2,4- DICHLORO-BENZOYL)-ISOPROPYL- AMINO]-THIOPHENE-2-CARBOXYLIC ACID +++ 377

3-[(2-ACETOXY-4-METHYL-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 378

3-[ISOPROPYL-(2-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 379

3-[ISOPROPYL-(2-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 380

3-(CYCLOHEPTANECARBONYL- ISOPROPYL-AMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 381

3-[ISOPROPYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-(3-TRIFLUOROMETHYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++ 382

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO-5-METHYL- THIOPHENE-2-CARBOXYLIC ACID +++ 383

3-[ISOPROPYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-(3-NITRO-PHENYL)-THIOPHENE-2- CARBOXYLIC ACID +++ 384

3-[(3-CYCLOPENTYL-PROPIONYL)- METHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 385

3-(BUTYRYL-METHYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 386

3-(METHYL-PENT-4-ENOYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 387

3-[ISOPROPYL-(5-METHYL-3-OXO-3H- ISOINDOL-1-YL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 388

3-[METHYL-(3-METHYL-BUTYRYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 389

3-(METHYL-PENTANOYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 390

3-[METHYL-(4-METHYL-PENTANOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 391

3-(CYCLOPENTANECARBONYL- ETHYL-AMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 392

3-[(3-CYCLOPENTYL-PROPIONYL)- ETHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 393

3-(CYCLOBUTANECARBONYL-ETHYL- AMINO)-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 394

3-(BUT-2-ENOYL-ETHYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 395

3-[ISOPROPYL-(4-METHYL-2-VINYL- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 396

3-[ISOPROPYL-(4-METHYL- CYCLOHEX-1-ENECARBONYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 397

3-(ALLYL-HEXANOYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 398

3-(ALLYL-CYCLOBUTANECARBONYL- AMINO)-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID + 399

3-(ALLYL-PENTANOYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 400

3-[ALLYL-(4-METHYL-PENTANOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 401

3-[ALLYL-(2-CYCLOPENTYL-ACETYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 402

3-[(2-HYDROXY-4-METHYL- CYCLOHEXANECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 403

3-[(2,4-DICHLORO-BENZOYL)-(1- PHENYL-ETHYL)-AMINO]-5-PHENYL THIOPHENE-2-CARBOXYLIC ACID +++ 404

3-[(2,4-DICHLORO-BENZOYL)-(1- PHENYL-ETHYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 405

3-[ISOPROPYL-(3-METHYL- CYCLOPENT-3-ENECARBONYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 406

3-[(2-BENZYLOXY-4-METHYL- BENZOYL)-ISOPROPYL-AMINO]-5-(3- TRIFLUOROMETHYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++ 407

3-[(2,4-DIMETHYL- CYCLOHEXANECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 408

3-[ISOPROPYL-(3-METHYL- CYCLOPENTANECARBONYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 409

3-[(2-HYDROXY-4-METHYL- CYCLOHEXANECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 410

5-PHENYL-3-[PROPIONYL-(4- TRIFLUOROMETHYL-BENZYL)- AMINO]-THIOPHENE-2-CARBOXYLIC ACID ++ 411

3-[ISOBUTYRYL-(4- TRIFLUOROMETHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 412

3-[(3-METHYL-BUTYRYL)-(4- TRIFLUOROMETHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 413

3-[CYCLOPROPANECARBONYL-(4- TRIFLUOROMETHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 414

3-[CYCLOBUTANECARBONYL-(4- TRIFLUOROMETHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 415

3-[BUTYRYL-(4-TRIFLUOROMETHYL- BENZYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 416

3-[(2-CYCLOPENTYL-ACETYL)-(4- TRIFLUOROMETHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 417

3-[(4-TERT-BUTYL-BENZYL)- PROPIONYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 418

3-(4-NITRO-BENZYL)-PROPIONYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 419

3-[(3-METHYL-BUTYRYL)-(4-NITRO- BENZYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 420

3-[CYCLOPROPANECARBONYL-(4- NITRO-BENZYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 421

3-[(2-CHLORO-BENZYL)-ISOBUTYRYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 422

3-[(2-CHLORO-BENZYL)-(3-METHYL- BUTYRYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 423

3-[(2-CHLORO-BENZYL)- CYCLOPROPANECARBONYL-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID + 424

3-[(ADAMANTANE-1-CARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID + 425

3-[(2-CHLORO-BENZYL)- CYCLOBUTANECARBONYL-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 426

3-[ACETYL-(2-METHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID + 427

3-[(2-METHYL-BENZYL)-PROPIONYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 428

3-[(2-HYDROXY-4-METHYL- BENZOYL)-ISOPROPYL-AMINO]-5-(3- TRIFLUOROMETHYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID ++ 429

3-[(1-ACETYL-PIPERIDIN-4-YL)-(2,4- DICHLORO-BENZOYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 430

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-[4-(1#H!- TETRAZOL-5-YL)-PHENYL]- THIOPHENE-2-CARBOXYLIC ACID +++ 431

3-[(2-CYANO-4-METHYL-BENZOYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 432

3-[CYCLOBUTANECARBONYL-(2- METHYL-BENZYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 433

3-[BUTYRYL-(2-METHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 434

3-[ACETYL-(3-METHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 435

3-[CYCLOBUTANECARBONYL-(4- METHYL-BENZYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 436

3-[CYCLOHEXANECARBONYL-(4- TRIFLUOROMETHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 437

3-[(4-TERT-BUTYL-BENZYL)- ISOBUTYRYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 438

3-[(4-TERT-BUTYL-BENZYL)- CYCLOPROPANECARBONYL-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 439

3-[(4-TERT-BUTYL-BENZYL)- CYCLOBUTANECARBONYL-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 440

3-[(4-TERT-BUTYL-BENZYL)- BUTYRYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 441

3-[(4-TERT-BUTYL-BENZYL)- CYCLOHEXANECARBONYL-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 442

3-[(4-TERT-BUTYL-BENZYL)-(2- CYCLOPENTYL-ACETYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 443

3-[(2-CYCLOPENTYL-ACETYL)-(4- NITRO-BENZYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 444

3-[(2-CHLORO-BENZYL)- CYCLOHEXANECARBONYL-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 445

3-[(2-CYCLOPENTYL-ACETYL)-(3- METHYL-BENZYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 446

3-[BUTYRYL-(3-METHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 447

3-[BUTYRYL-(2-CHLORO-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 448

3-[ISOPROPYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-#M!-TOLYL-THIOPHENE-2- CARBOXYLIC ACID +++ 449

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-THIAZOL-2-YL- THIOPHENE-2-CARBOXYLIC ACID +++ 450

3-(ACETYL-BENZYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 451

3-(BENZYL-PROPIONYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 452

3-[BENZYL-(2-METHOXY-ACETYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 453

3-[BENZYL-(3-METHYL-BUTYRYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 454

3-(BENZYL- CYCLOPROPANECARBONYL-AMINO)- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 455

3-[ACETYL-(4-CHLORO-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 456

3-[(4-CHLORO-BENZYL)-PROPIONYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 457

3-[(4-CHLORO-BENZYL)-ISOBUTYRYL- AMINO-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 458

3-[(4-CHLORO-BENZYL)-(3-METHYL- BUTYRYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 459

3-[(4-CHLORO-BENZYL)- CYCLOPROPANECARBONYL-AMINO]- 5-PHENYL-′THIOPHENE-2- CARBOXYLIC ACID ++ 460

5-(4-ACETYL-PHENYL)-3-[ISOPROPYL- (4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 461

3-[(4-CHLORO-BENZYL)- CYCLOBUTANECARBONYL-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 462

3-[BUTYRYL-(4-CHLORO-BENZYL)- AMINO-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 463

3-[(4-CHLORO-BENZYL)-(2- CYCLOPENTYL-ACETYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 464

3-[ACETYL-(4-TRIFLUOROMETHYL- BENZYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 465

3-[ISOBUTYRYL-(3-METHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 466

3-[CYCLOPROPANECARBONYL-(3- METHYL-BENZYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 467

3-[(4-METHYL-BENZYL)-PROPIONYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 468

3-[ISOBUTYRYL-(4-METHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 469

3-[CYCLOPROPANECARBONYL-(4- METHYL-BENZYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 470

3-[BUTYRYL-(4-METHYL-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 471

3-[(3-METHOXY-BENZYL)- PROPIONYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID + 472

3-[(3-METHOXY-BENZYL)-(3-METHYL- BUTYRYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 473

3-[CYCLOBUTANECARBONYL-(3- METHOXY-BENZYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 474

3-[(2-CARBAMOYL-4-METHYL- BENZOYL)-ISOPROPYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 475

3-[BUTYRYL-(3-METHOXY-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 476

3-[ACETYL-(3-CHLORO-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID + 477

3-[(3-CHLORO-BENZYL)-PROPIONYL- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 478

3-[(3-CHLORO-BENZYL)-(2-METHOXY- ACETYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID + 479

3-[(3-CHLORO-BENZYL)-(3-METHYL- BUTYRYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 480

3-[(3-CHLORO-BENZYL)- CYCLOPROPANECARBONYL-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 481

3-[(3-CHLORO-BENZYL)- CYCLOBUTANECARBONYL-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 482

3-[BUTYRYL-(3-CHLORO-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 483

3-[ACETYL-(2,4-DIFLUORO-BENZYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 484

3-[(2,4-DIFLUORO-BENZYL)-(2- METHOXY-ACETYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 485

3-[(2,4-DIFLUORO-BENZYL)- ISOBUTYRYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 486

3-[(2,4-DIFLUORO-BENZYL)-(3- METHYL-BUTYRYL)-AMINO-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 487

3-[BENZYL-(2-CYCLOPENTYL- ACETYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 488

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-(1H-INDOL-5- YL)-THIOPHENE-2-CARBOXYLIC ACID +++ 489

3-(BENZYL- CYCLOBUTANECARBONYL-AMINO)- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 490

3-[CYCLOHEXANECARBONYL-(2,4- DIFLUORO-BENZYL)-AMINO-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 491

3-{ALLYL-[2-(4-METHOXY-PHENYL)- ACETYL]-AMINO}-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID + 492

3-(ETHYL-HEXANOYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 493

3-(BUTYRYL-ETHYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 494

3-[ETHYL-(4-METHYL-PENTANOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 495

3-[CYCLOBUTANECARBONYL-(2,4- DIFLUORO-BENZYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 496

3-[BUTYRYL-(2,4-DIFLUORO- BENZYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 497

3-(CYCLOPENTANECARBONYL- METHYL-AMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 498

3-(CYCLOHEXANECARBONYL- METHYL-AMINO)-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 499

3-[(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YL)-(2,4-DICHLORO-BENZOYL)- AMINO]-PYRROLIDINE-1- CARBOXYLIC ACID #TERT!-BUTYL ESTER +++ 500

3-[(1,4-DIMETHYL- CYCLOHEXANECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 501

5-(4-ETHYL-PHENYL)-3-[(2-HYDROXY- 4-METHYL-BENZOYL)-ISOPROPYL- AMINO]-THIOPHENE-2-CARBOXYLIC ACID +++ 502

3-[(2-HYDROXY-4-METHYL- BENZOYL)-ISOPROPYL-AMINO]-5-#M!- TOLYL-THIOPHENE-2-CARBOXYLIC ACID +++ 503

3-[(2,4-DICHLORO-BENZOYL)- PYRROLIDIN-3-YL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 504

4-{5-CARBOXY-4-[(2,4-DICHLORO- BENZOYL)-ISOPROPYL-AMINO]- THIOPHEN-2-YL}-3,6-DIHYDRO-2#H!- PYRIDINE-1-CARBOXYLIC ACID BENZYL ESTER +++ 505

3-{[2-(HYDROXYIMINO-METHYL)-4- METHYL-BENZOYL]-ISOPROPYL- AMINO}-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 506

3-[(1-CARBAMIMIDOYL-PIPERIDIN-4- YL)-(2,4-DICHLORO-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 507

4-[(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YL)-(2,4-DICHLORO-BENZOYL)- AMINO]-AZEPANE-1-CARBOXYLIC ACID TERT!-BUTYL ESTER +++ 508

4-{[(2-CARBOXY-5-PHENYL- THIOPHEN-3-YL)-(2,4-DICHLORO- BENZOYL)-AMINO]-METHYL}- PIPERIDINE-1-CARBOXYLIC ACID BENZYL ESTER +++ 509

3-[AZEPAN-4-YL-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 510

3-[(4-METHYL- CYCLOHEXANECARBONYL)- PIPERIDIN-4-YL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID LITHIUM SALT ++ 511

3-[(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YL)-(2,4-DICHLORO-BENZOYL)- AMINO]-PIPERIDINE-1-CARBOXYLIC ACID #TERT!-BUTYL ESTER +++ 512

3-[(4-BENZYLOXYCARBONYLAMINO- CYCLOHEXYL)-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 513

3-[ISOPROPYL-(4-METHYL-2-OXO- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 514

3-[(2,4-DICHLORO-BENZOYL)- PIPERIDIN-3-YL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID; COMPOUND WITH GENERIC INORGANIC NEUTRAL COMPONENT +++ 515

3-[(4-BENZYLOXYCARBONYLAMINO- CYCLOHEXYL)-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 516

3-[(2-BENZYLOXY-1-METHYL-ETHYL)- (2,4-DICHLORO-BENZOYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 517

3-[(2,2-DIMETHYL-[1,3]DIOXAN-5-YL)- (4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 518

3-[(2,4-DICHLORO-BENZOYL)-(2- HYDROXY-1-HYDROXYMETHYL- ETHYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 519

3-[(2,4-DICHLORO-BENZOYL)- PIPERIDIN-4-YLMETHYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 520

3-[(2-CHLORO-BENZOYL)-PIPERIDIN-4- YLMETHYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 521

3-[(4,6-DICHLORO-1#H!-INDOLE-2- CARBONYL)-ISOPROPYL-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 522

3-[(2,4-DICHLORO-BENZOYL)-(2- HYDROXY-1-METHYL-ETHYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 523

4-{1-[(2-CARBOXY-5-PHENYL- THIOPHEN-3-YL)-(2,4-DICHLORO- BENZOYL)-AMINO]-ETHYL}- PIPERIDINE-1-CARBOXYLIC ACID BENZYL ESTER ++ 524

4-{5-CARBOXY-4-[ISOPROPYL-(4- METHYL- CYCLOHEXANECARBONYL)-AMINO]- THIOPHEN-2-YL}-3,6-DIHYDRO-2#H!- PYRIDINE-1-CARBOXYLIC ACID BENZYL ESTER +++ 525

3-[(4-METHYL- CYCLOHEXANECARBONYL)-PYRIDIN- 4-YL-AMINO]-5-PHENYL-THIOPHENE- 2-CARBOXYLIC ACID +++ 526

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-5-PIPERIDIN-4- YL-THIOPHENE-2-CARBOXYLIC ACID; COMPOUND WITH TRIFLUORO- ACETIC ACID + 527

3-[ISOPROPYL-(4-PROPYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 528

4-[(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YL)-(2,4-DICHLORO-BENZOYL)- AMINO]-CYCLOHEXYL-AMMONIUM; TRIFLUORO-ACETATE +++ 529

3-[(2,4-DICHLORO-BENZOYL)-(1- PIPERIDIN-4-YL-ETHYL)-AMINO]-5- PHENYL-THIOPHENE-2- CARBOXYLIC ACID; COMPOUND WITH TRIFLUORO-ACETIC ACID +++ 530

3-[(CYCLOHEX-3-ENECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ++ 531

3-[(4-ETHYL- CYCLOHEXANECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 532

3-[(4-CHLORO- CYCLOHEXANECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 533

4-[(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YL)-(2,4-DICHLORO-BENZOYL)- AMINO]-3-METHYL- PIPEPIDINE-1-CARBOXYLIC ACID BENZYL ESTER +++ 534

3-[(2,4-DICHLORO-BENZOYL)-(2- METHOXY-CYCLOHEXYL)-AMINO]-5- PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 535

3-[(2,4-DICHLORO-BENZOYL)-(2,2- DIMETHYL-[1,3]DIOXAN-5-YL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 536

3-[ISOPROPYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-(1-METHYL-PIPERIDIN-4-YL)- THIOPHENE-2-CARBOXYLIC ACID +++ 537

3-[(2,4-DICHLORO-BENZOYL)-(3- METHYL-PIPERIDIN-4-YL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID; COMPOUND WITH TRIFLUORO- ACETIC ACID +++ 538

3-[(2,4-DICHLORO-BENZOYL)-(2- HYDROXY-CYCLOHEXYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 539

4-{[(2-CARBOXY-5-PHENYL- THIOPHEN-3-YL)-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- METHYL}-PIPERIDINE-1-CARBOXYLIC ACID BENZYL ESTER +++ 540

3-[((1R,2S,4R)-2-HYDROXY-4-METHYL- CYCLOHEXANECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 541

3-{ISOPROPYL-[1-(4-METHOXY-2,3,6- TRIMETHYL-BENZENESULFONYL)-5- METHYL-1,2,3,6-TETRAHYDRO- PYRIDINE-2-CARBONYL]-AMINO}-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 542

3-[(2,4-DICHLORO-BENZOYL)- ISOPROPYL-AMINO]-4-FLUORO-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 543

3-[(2,4-DICHLORO-BENZOYL)-(1- METHYL-PIPERIDIN-4-YL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 544

4-{[(2-CARBOXY-5-PHENYL- THIOPHEN-3-YL)-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- METHYL}-PIPERIDINIUM; TRIFLUORO-ACETATE +++ 545

3-[(2-TERT- BUTOXYCARBONYLAMINO-1- METHYL-ETHYL)-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 546

2-[(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YL)-(2,4-DICHLORO-BENZOYL)- AMINO]-PROPYL-AMINE TRIFLUOROACETIC ACID SALT +++ 547

3-[(3-CARBOXY-CYCLOPENTYL)-(2,4- DICHLORO-BENZOYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 548

3-[(3-CARBOXY-CYCLOPENTYL)-(2,4- DICHLORO-BENZOYL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 549

2-[(2-CARBOXY-5-PHENYL-THIOPHEN- 3-YL)- (2,4-DICHLORO-BENZOYL)-AMINO]- CYCLOHEXYL- AMMONIUM; CHLORIDE +++ 550

3-(BENZOYL-METHYL-AMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID + 551

{[5-PHENYL-3-(TOLUENE-4- SULFONYLAMINO)-THIOPHENE-2- CARBONYL]-AMINO}-ACETIC ACID ++ 552

5-BROMO-3-(TOLUENE-2- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID ++ 553

3-[CYCLOHEXYL-(2,4-DICHLORO- BENZOYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID ′+++ 554

3-[[1,3]DIOXAN-5-YL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 555

3-[[2-(TERT-BUTYL-DIMETHYL- SILANYLOXY)-1-METHYL-2-PHENYL- ETHYL]-(2,4-DICHLORO-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 556

3-[[2-(TERT-BUTYL-DIMETHYL- SILANYLOXY)-1-METHYL-2-PHENYL- ETHYL]-(2,4-DICHLORO-BENZOYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID ++ 557

3-[(2,4-DICHLORO-BENZOYL)-(2- DIETHYLAMINO-THIAZOL-5- YLMETHYL)-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 558

(5-{[(2-CARBOXY-5-PHENYL- THIOPHEN-3-YL)-(2,4-DICHLORO- BENZOYL)-AMINO]-METHYL}- THIAZOL-2-YL)-DIETHYL- AMMONIUM; CHLORIDE +++ 559

5-(4-FLUORO-PHENYL)-3-[ISOPROPYL- (4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 560

3-[((1S,2R,4S)-2-HYDROXY-4-METHYL- CYCLOHEXANECARBONYL)- ISOPROPYL-AMINO]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 561

3-[(2,4-DICHLORO-BENZOYL)-(2- METHOXY-1-METHYL-ETHYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 562

3-[(4S)-ISOPROPYL-(4-METHYL- CYCLOHEX-1-ENECARBONYL)- AMINO]-5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 563

5-(4-CHLORO-PHENYL)-3-(TOLUENE-4- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID AMIDE ++ 564

5-(4-FLUORO-PHENYL)-3-(TOLUENE-4- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID AMIDE ++ 565

5-(4-METHOXY-PHENYL)-3-(TOLUENE- 4-SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID AMIDE ++ 566

3-METHYL-(4-METHYLBENZOYL)- AMINO)5-PHENYL THIOPHENE-2- CARBOXYLIC ACID (2-HYDROXY- ETHYL)AMIDE ++ 567

5-PHENYL-3-(TOLUENE-4- SULFONYLAMINO)-THIOPHENE-2- CARBOXYLIC ACID CYCLOBUTYLAMIDE ++ 568

3-(2,4-DIMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID AMIDE ++ 569

5-BROMO-3-[(2,4-DICHLORO- BENZOYL)-ISOPROPYL-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 570

5-(4-CHLORO-PHENYL)-3-[ISOPROPYL- (4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 571

5-(4′-CHLORO-BIPHENYL-4-YL)-3- [ISOPROPYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 572

3-[(4-METHYL- CYCLOHEXANECARBONYL)- (TETRAHYDRO-PYRAN-4-YL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 573

3-[(4-METHYL- CYCLOHEXANECARBONYL)-(1- METHYL-PIPERIDIN-4-YL)-AMINO]-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID +++ 574

3-[(4-METHYL- CYCLOHEXANECARBONYL)- PIPERIDIN-4-YL-AMINO]-5-PHENYL- THIOPHHNE-2-CARBOXYLIC ACID + 575

′3-[ISOPROPYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-(4-TRIFLUOROMETHYL-PHENYL)- THIOPHENE-2-CARBOXYLIC ACID +++ 576

5-(4-CYANO-PHENYL)-3-[ISOPROPYL- (4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- THIOPHENE-2-CARBOXYLIC ACID +++ 577

′3-[ISOPROPYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-(4-METHOXY-PHENYL)-THIOPHENE- 2-CARBOXYLIC ACID +++ 578

3-[(2-METHOXY-1-METHYL-ETHYL)-(4- METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 579

3-[CYCLOHEXYL-(4-METHYL- CYCLOHEXANECARBONYL)-AMINO]- 5-PHENYL-THIOPHENE-2- CARBOXYLIC ACID +++ 580

3-(4-CHLORO-2,5 DIMETHYL- BENZENESULFONYLAMINO)-5- PHENYL-THIOPHENE--2-CARBOXYLIC ACID AMIDE +++ 583

3-[(2,4-DICHLORO-PHENYL)- ISOPROPYL-CARBAMOYL]-5-PHENYL- THIOPHENE-2-CARBOXYLIC ACID +++ 584

3-(METHYL-P-TOLYL-CARBAMOYL)-5- PHENYL-THIOPHENE-2-CARBOXYLIC ACID ++ 585

3-[(2,4-DICHLORO-PHENYL)-METHYL- CARBAMOYL]-5-PHENYL-THIOPHENE- 2-CARBOXYLIC ACID ++ *: +++ IC₅₀ <5 μM ++ IC₅₀ 5 μM-20 μM + IC₅₀ > 20 μM

Example 28 List of Compounds Having Anti-Helicase Activity *

Anti-ATPase Anti-ATPase (Malachite (HPLC Compound # Compound name Structure Green assay) EC₅₀ method) EC₅₀ Compound #14  3-(4-Chloro-2,5-dimethyl- benzenesulfonylamino)-5- (4-chloro-phenyl)- thiophene-2- carboxylic acid

+ ND Compound #19  3-(4-Chloro-2,5-dimethyl- benzenesulfonylamino)-5- (4-isobutyl-phenyl)- thiophene-2-carboxylic acid

+++ ++ Compound #223 3-(4-Bromo-2- fluorobenzenesulfo- nylamino)-5-(4- isobutylphenyl)-thiophene- 2-carboxylic acid

ND +++ Compound #224 3-(4-Bromo-2- methylbenzenesulfo- nylamino)-5-(4- isobutylphenyl)-thiophene- 2-carboxylic acid

ND ++ Compound #225 5-(4-Isobutylphenyl 3-(3- methoxy-benzenesulfonyl- amino)-thiophene-2- carboxylic acid

ND + Compound #581 5-(4-Isobutyl-phenyl)-3-[5- (5-trifluoromethyl-isoxazol- 3-yl)-thiophene-2- sulfonylamino]-thiophene-2- carboxylic acid

ND ++ Compound #227 3-[2,5-Bis-(2,2,2- trifluoroethoxy)- benzenesulfonylamino]-5- (4-isobutyl-phenyl)- thiophene-2-carboxylic acid

ND + Compound #228 3-(2-Chloro-4- cyanobenzenesulfonylamino)- 5-(4-isobutylphenyl)- thiophene-2-carboxylic acid

ND + Compound #582 5-(4-Isobutyl-phenyl)-3- (2,3,4-trifluoro- benzenesulfonylamino)- thiophene-2-carboxylic acid

ND + *+++ IC₅₀ < 5 μM ++ IC₅₀ 5 μM-20 μM + IC₅₀ > 20 μM 

1.-97. (canceled)
 98. A compound of formula I:

or a pharmaceutically acceptable salt thereof; wherein, X is

M is

J is

Y¹ is a bond; Y is COOH; R₁ is C₂₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl or C₆₋₁₈ aralkyl; R₂ is C₂₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈ aralkyl; R₃ is H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl or C₆₋₁₈ aralkyl; and Z is H, halogen, or C₁₋₆ alkyl; with the proviso that: when X is Benzamide and R₁ is phenyl, then R₃ is other than hydrogen; wherein alkyl in R₁, R₂, R₃, and Z is in each case optionally substituted by one or more of: halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; alkenyl in R₁, R₂, and R₃ is in each case optionally substituted by one or more of: halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; alkynyl in R₁, R₂, and R₃ is in each case optionally substituted by one or more of: halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; aryl in R₁, R₂, and R₃ is in each case optionally substituted by one or more of: halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; aralkyl in R₁, R₂, and R₃ is in each case an aryl group attached to the adjacent atom by a C₁₋₆ alkyl, C₂₋₆ alkenyl, or C₂₋₆ alkynyl, and the aryl group is in each case optionally substituted by one or more of: halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; heterocycle in R₁, R₂, and R₃ is in each case optionally substituted by one or more of: halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; heteroaralkyl in R₁, R₂, and R₃ in each case an heterocycle group attached to the adjacent atom by a C₁₋₆ alkyl, C₂₋₆ alkenyl, or C₂₋₆ alkynyl, and the heterocycle group is in each case optionally substituted by one or more of: halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino or guanido; and R₁₂, R_(c), R_(d), R₁₃ and R₁₄, are each independently H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈ aralkyl, or R_(c) and R_(d) are taken together with the oxygens to form a 5 to 10 membered heterocycle, or R₁₃ and R₁₄ are taken together with the nitrogen to form a 3 to 10 membered heterocycle, in each case above alkyl groups include alkyls in which one or more hydrogen atoms are each replaced by a halogen, and sulfur atoms are in the form of S, SO, or SO₂.
 99. A compound according to claim 98, wherein said compound is of formula Ia:

or a pharmaceutically acceptable salt thereof.
 100. A compound according to claim 98, wherein X is


101. A compound according to claim 98, wherein Z is H.
 102. A compound according to claim 98, wherein R₁ is C₂₋₁₂ alkyl, C₆₋₁₄ aryl, or C₃₋₁₂ heterocycle, which in each case is substituted or unsubstituted.
 103. A compound according to claim 98, wherein R₃ is H, C₁₋₁₂ alkyl, C₆₋₁₈ aralkyl, C₃₋₁₂ heterocycle, or C₃₋₁₈ heteroaralkyl, which in each case is substituted or unsubstituted.
 104. A compound according to claim 98, wherein R₃ is H, or R₃ is methyl, ethyl, isopropyl, cyclopropyl, cyclohexyl, allyl, piperidinyl, piperazinyl, pyrrolidinyl, azetidinyl, aziridinyl, pyridinyl, piperidinylmethyl, dioxanyl, azepanyl or benzyl, which in each case is unsubstituted or substituted by one or more substituents chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino, and guanido; and R₁₂, R_(c), R_(d), R₁₃ and R₁₄ are each independently H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈ aralkyl, or R_(c) and R_(d), taken together with the oxygens, form a 5 to 10 membered heterocycle, or R₁₃ and R₁₄, taken together with the nitrogen, form a 3 to 10 membered heterocycle.
 105. A compound according to claim 104, wherein R₃ is isopropyl or cyclohexyl, which in each case is substituted or unsubstituted.
 106. A compound according to claim 98, wherein R₂ is C₂₋₁₂ alkyl, C₆₋₁₄ aryl, or C₃₋₁₂ heterocycle, which in each case is substituted or unsubstituted.
 107. A compound according to claim 98, wherein R₂ is cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cycloheptyl, 2-(cyclopentyl)-ethyl, ethyl, vinyl, propyl, propenyl, isopropyl, butyl, butenyl isobutyl, pentyl, neopentyl or t-butyl, which in each case is unsubstituted or substituted by one or more substituents chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino, and guanido; and R₁₂, R_(c), R_(d), R₁₃ and R₁₄ are each independently H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈ aralkyl, or R_(c) and R_(d), taken together with the oxygens, form a 5 to 10 membered heterocycle, or R₁₃ and R₁₄, taken together with the nitrogen, form a 3 to 10 membered heterocycle.
 108. A compound according to claim 99, wherein X is


109. A compound according to claim 99, wherein Z is H.
 110. A compound according to claim 99, wherein R₁ is C₂₋₁₂ alkyl, C₆₋₁₄ aryl, or C₃₋₁₂ heterocycle, which in each case is substituted or unsubstituted.
 111. A compound according to claim 99, wherein R₃ is H, or R₃ is C₁₋₁₂ alkyl, C₆₋₁₈ aralkyl, C₃₋₁₂ heterocycle, or C₃₋₁₈ heteroaralkyl, which in each case is substituted or unsubstituted.
 112. A compound according to claim 99, wherein R₃ is H, or R³ is methyl, ethyl, isopropyl, cyclopropyl, cyclohexyl, allyl, piperidinyl, piperazinyl, pyrrolidinyl, azetidinyl, aziridinyl, pyridinyl, piperidinylmethyl, dioxanyl, azepanyl or benzyl, which in each case is unsubstituted or substituted by one or more substituents chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino, and guanido; and R₁₂, R_(c), R_(d), R₁₃ and R₁₄ are each independently H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈ aralkyl, or R_(c) and R_(d), taken together with the oxygens, form a 5 to 10 membered heterocycle, or R₁₃ and R₁₄, taken together with the nitrogen, form a 3 to 10 membered heterocycle.
 113. A compound according to claim 99, wherein R₃ is H, or R₃ is methyl, isopropyl, piperidinyl, piperidinylmethyl, dioxolanyl or cyclohexyl, which in each case is substituted or unsubstituted.
 114. A compound according to claim 99, wherein R₂ is C₂₋₁₂ alkyl, C₆₋₁₄ aryl, or C₃₋₁₂ heterocycle, which in each case is substituted or unsubstituted.
 115. A compound according to claim 99, wherein Z is H and X is


116. A compound according to claim 115, wherein R₁ is C₂₋₁₂ alkyl, C₆₋₁₄ aryl, or C₃₋₁₂ heterocycle, which in each case is substituted or unsubstituted; R₃ is H, or R³ is C₁₋₁₂ alkyl, C₆₋₁₈ aralkyl, C₃₋₁₂ heterocycle, or C₃₋₁₈ heteroaralkyl, which in each case is substituted or unsubstituted; and R₂ is C₂₋₁₂ alkyl, C₆₋₁₄ aryl, or C₃₋₁₂ heterocycle, which in each case is substituted or unsubstituted.
 117. A compound according to claim 115, wherein R₃ is H, or R³ is methyl, ethyl, isopropyl, cyclopropyl, cyclohexyl, allyl, piperidinyl, piperazinyl, pyrrolidinyl, azetidinyl, aziridinyl, pyridinyl, piperidinylmethyl, dioxanyl, azepanyl or benzyl, which in each case is unsubstituted or substituted by one or more substituents chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino, and guanido; R₂ is cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl cyclohexyl, cycloheptyl, 2-(cyclopentyl)-ethyl, ethyl, vinyl, propyl, propenyl, isopropyl, butyl, butenyl isobutyl, pentyl, neopentyl or t-butyl, which in each case is unsubstituted or substituted by one or more substituents chosen from halogen, nitro, nitroso, SO₃R₁₂, PO₃R_(c)R_(d), CONR₁₃R₁₄, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₆₋₁₂ aralkyl, C₆₋₁₂ aryl, C₁₋₆ alkyloxy, C₂₋₆ alkenyloxy, C₂₋₆ alkynyloxy, C₆₋₁₂ aryloxy, C(O)C₁₋₆ alkyl, C(O)C₂₋₆ alkenyl, C(O)C₂₋₆ alkynyl, C(O)C₆₋₁₂ aryl, C(O)C₆₋₁₂ aralkyl, C₃₋₁₀ heterocycle, hydroxyl, NR₁₃R₁₄, C(O)OR₁₂, cyano, azido, amidino, and guanido; and R₁₂, R_(c), R_(d), R₁₃ and R₁₄ are each independently H, C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₂₋₁₂ alkynyl, C₆₋₁₄ aryl, C₃₋₁₂ heterocycle, C₃₋₁₈ heteroaralkyl, or C₆₋₁₈ aralkyl, or R_(c) and R_(d), taken together with the oxygens, form a 5 to 10 membered heterocycle, or R₁₃ and R₁₄, taken together with the nitrogen, form a 3 to 10 membered heterocycle.
 118. A method for treating a Hepatitis C virus (HCV) infection in a host comprising administering to the host a therapeutically effective amount of at least one compound according to claim
 98. 